Frontispiece: In‐Sequence High‐Specificity Dual‐Reporter Unlocking of Fluorescent Probe Enables the Precise Identification of Atherosclerotic Plaques

Abstract

Bioanalytics. In their Research Article (e202204518), Dingbin Liu et al. designed a dual‐reporter unlocking platform, termed iSHERLOCK, to detect lipid droplets and HClO via fluorescent readouts. Based on iSHERLOCK, two hallmarks of atherosclerosis were quantitatively measured.

Fluorescent Probes for Disease Diagnosis

“…In 2022, the Liu group reported probe 137, a dual-targeting sequential fluorescence system to precisely identify atherosclerotic plaque in vivo and in vitro, which they called insequence high-specificity dual-reporter unlocking (iSHER-LOCK) (Figure 66). 222 iSHERLOCK detects both HClO and lipid droplets, two hallmarks of atherosclerosis. In a manner similar to previous probes featured in this review, this probe is Two NIR fluorescent probes 138 and 139 were reported by the Tang group in 2019 for the photoacoustic imaging of atherosclerotic plaque vulnerability, looking specifically at plaque oxidative stress/inflammatory activity.…”

“…In 2022, the Liu group reported probe 137 , a dual-targeting sequential fluorescence system to precisely identify atherosclerotic plaque in vivo and in vitro , which they called in-sequence high-specificity dual-reporter unlocking (iSHERLOCK) ( Figure 66 ). 222 iSHERLOCK detects both HClO and lipid droplets, two hallmarks of atherosclerosis. In a manner similar to previous probes featured in this review, this probe is nonfluorescent in aqueous media, “switching on” on entering nonpolar lipid droplets, with HClO then triggering oxidation to shift the fluorescence signal to create the dual output.…”

“…FY and FR represent the fluorescent intensities (FI) in the yellow and red channels, respectively. Reproduced with permission from ref ( 222 ). Copyright 2022 John Wiley & Sons.…”