Cloud point technique used effectively for extraction and pre-concentration of iron(III) in the urine samples of occupational workers prior measured by using flame atomic absorption spectrometry and UV-visible spectrophotometer. The metal responds with benzidine as reagent in a non-ionic surfactant Triton X-114 medium. The main factors affecting cloud point extraction efficiencies, such as pH of sample solution, concentration of benzidine reagent, type of surfactant, concentration of Triton X-114, effect of salt out, influence of interferences and impact of equilibration temperature and time were studied. The calibration curve was linear in the range of 0.25-3.0 µg mL -1 with r 2 = 0.9655 for UV-visible spectrophotometer at λmax 425 nm. The limit of detection was 0.25 µg mL -1 . The relative standard deviation for six replicates was 3.071 %.
The cloud point technique was effectively utilized for extraction and pre-concentration of nickel(II) in urine samples before measurement by UV-Vis spectrophotometer and AAS techniques. The metal response to a para-aminophenol (PAP) reagent in a non-ionic surfactant Triton X-114 medium was to form the Ni-PAP complex. The adopted concentration for PAP, concentration of Triton X-114, pH effect and water bath temperature, incubation time, salt effect, and interference effects were all optimized. The calibration curve was linear over the range of (0.0625–1.25) mg L–1 with a correlation coefficient r2 of 0.9682 for the UV-Vis spectrophotometer at a λmax of 629 nm. The limit of detection was 0.005 mg/L. The relative standard deviation for six replicates was 1.07%. This method was applied successfully to determine copper (II) concentrations in 44 urine samples of occupational worker samples as determined by UV-Vis spectrophotometry and FAAS techniques.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.