The microbial community on a host relies on its immune status and pathophysiological condition. Diabetes mellitus is a metabolic disorder associated with a 25% increased risk of developing foot infection. The pathophysiological differences between diabetic foot infection (DFI) and non-DFI patients may alter the microbial composition in infections. The present study aims to comparatively analyze the microbes colonized in DFI and non-DFI patients in Bangladesh. Pus specimens were collected from 67 DFI and 12 non-DFI patients to investigate the bacteria associated with foot infection. For this investigation, an array of microbiological, molecular biological and immunological approaches were performed. Common bacteria detected in both DFI/non-DFI samples were Pseudomonas spp. (22/29%), Bacillus spp. (12/3%), Enterobacter spp. (22/7%), Staphylococcus spp. (13/13%) and Acinetobacter spp. (10/10%). Enterococcus spp. (9%) and Klebsiella spp. (8%) occurred only in DFI patients, whereas Citrobacter spp. (29%) was only detected in non-DFI samples. The rate of occurrence of three organisms, namely, Enterococcus spp. |Z|=2.2125, Klebsiella spp. |Z|=1.732, Bacillus spp. |Z|=1.9034, were also statistically significant. Most of the isolates from DFI patients were commonly resistant to the cephalosporin (Ceftazidime, Ceftriazone, Cefurozime) and monobactam (Aztreonam) groups of antibiotics. DFI patients had comparatively higher C-reactive protein (CRP) levels than non-DFI patients, and a positive correlation was observed between multi-antibiotic resistance and CRP levels (one of the markers of chronic subclinical inflammation). The present investigation implicated a complex association of the bacterial population in DFI compared with non-DFI with different antimicrobial resistance properties, which was linked with CRP levels.
Blood agar is needed for culture of various organisms. As sheep blood is needed for it’s preparation, many small laboratories specially those of rural areas can not prepare it due to difficulty in collection of sheep blood. So, either they do not do the culture or do it without blood agar which cause missing of some important organisms. In this study Brain heart infusion agar was used together with Blood agar to assess it’s efficacy as an alternative of Blood agar. In total 1256 various samples were cultured, on Blood agar , Brain heart Infusion agar and MacConkey agar. Out of 1256 samples 404 samples showed growth of various organisms. It was noted that all bacteria including Enterococcus sp. grew equally in blood agar and BHIA. Brain heart infusion agar can be used as a surrogate of blood agar. Bangladesh J Med Microbiol 2018; 12 (1): 24-26
Rapid distribution of bacteria in wound infection and their appropriate treatment with antibiotics are crucial for health care providers. Bangladesh is vulnerable to multi-drug-registrant antibiotics due to extensive antibiotic misuse and other factors. The purpose of the present study was to see the frequency and distribution of bacteria isolated from pus and sensitivity patterns among hospitalized patients. A cross-sectional study was carried out from June 2020 to July 2021. The pus samples were collected from the patients who visited BIHS General Hospital, Dhaka. Isolation and Identification of bacteria were made by culture and biochemical test and antibiotic susceptibility test was done by disc diffusion method. The most common isolates were . Gram-positive bacteria were mostly resistant to Penicillin, Cefoxitin, Ampicillin, Azithromycin, Cotrimoxazole, Cefuroxime, and Cepradine antibiotics. 57.1% of and (100%) of was Methicillin-resistant, AMPC β-Lactamase producing bacteria (52.2%), and ESBL are (13.0%).Due to the abuse of antibiotics, Methicillin-resistant AMPC β-Lactamase, ESBL are increasing day by day. Our study found that MDR bacteria is increasing rapidly and which is a major problem. Therefore, Antibiotic susceptibility pattern testing is required before the use of antibiotics, and continuous monitoring of antibiotic sensitivity is needed to minimize resistance.
Pseudomonas aeruginosa is one of the most widespread gram-negative microorganisms identified in the clinical samples and most common causes of hospital acquired infection. P. aeruginosa is affecting both indoor and outdoor patients throughout the world. Due to frequent mutation in P. aeruginosa highly resistant strain developed rapidly. The aim of the study to determine the prevalence of P. aeruginosa species in different samples isolated from a Tertiary care Hospital as well as determination their diverse antibiotic resistance pattern. This cross-sectional study was carried out to determine in-vitro resistance pattern of P. aeruginosa isolates to common antimicrobial agents by disc diffusion method. Various clinical samples were collected from Bangladesh Health Sciences Hospital (BIHS) General Hospital, Dhaka. This research was carried out in the Department of Microbiology of Bangladesh University of Health Sciences (BUHS). Isolation, identification and antibiogram were performed for P. aeruginosa following standard microbiological laboratory procedure. A total of 218 P. aeruginosa were isolated from 3062 different clinical specimens which are statistically significant (p<0.0001). Among the highest number of P. aeruginosa were isolated from outdoor patients 140 compare to Indoor patients which are significantly higher (p <0.013). In this study Male (68.3%) are more vulnerable to P. aeruginosa infection compare to females (31.7%) which is also statistically significant. Young people (less than 35 years) were more susceptible to P. aeruginosa infection which is also statistically significant (p< 0.01). The highest number of P. aeruginosa was isolated from wound (43.12%), followed by pus (40.33%), sputum (8.71%) urine (7.80%). The maximum number of P. aeruginosa in various samples was resistant to aztreonam and co-tromoxazole followed by cephalosporins, aminoglycosides, carbapenems. The most sensitive antibiotic was colistin of followed by gentamycin and tetracycline. To control the spread of resistant bacteria, it is disparagingly vital to have stringent antibiotic guidelines. The antibiotic susceptibility pattern of P. aeruginosa requires to be continuously monitored in specialized clinical units and the results readily made available to the clinicians to minimize the resistance.
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