In this study, a detailed phytochemical analysis of the medicinal herb Inula viscosa leaves was performed. Furthermore, in vitro antioxidant and antifungal properties of its methanolic extract were evaluated and compared with the corresponding phenolic profile obtained by high-performance liquid chromatography and mass spectrometry. Data obtained underscore the high amount of total lipids (6.14%) in leaves. Chromatographic analysis revealed its high content of unsaturated fatty acids (UFAs) with the essential ones α-linolenic and linoleic acids being the main compounds. It also showed good nutritional quality because of its high UFA/saturated fatty acid ratio and the lower values of atherogenic and thrombogenic indices. The volatile oil analyzed by gas chromatography-mass spectrometry showed the abundance of nonterpenic compounds, namely aliphatic alkanes. The total phenol content (TPC) and total flavonoid content (TFC) were higher in I. viscosa leaves (103 mg GAE/g dw and 99 mg CE/g dw for TPC and TFC, respectively). The use of HPLC-PDA-ESI-MS/MS allowed the identification of 17 components with hydroxycinnamic acids, namely mono-and dicaffeoylquinic acids being the most prominent components. The presence of these phenolic compound conferred strong free radical scavenging and antifungal properties to the methanol extract. Therefore, I. viscosa leaves could be considered as an excellent source of food functional ingredients with high nutritional value and health benefits.
IntroductionProcalcitonin (PCT) biomarker is suggested to tailor antibiotic therapy in the medical intensive care unit (ICU) but studies in perioperative medicine are scarce. The aim of this study was to determine whether PCT reported thresholds are associated with the initial treatment response in perioperative septic shock secondary to intra-abdominal infection.MethodsThis single ICU, observational study included patients with perioperative septic shocks secondary to intra-abdominal infection. Demographics, PCT at days 0, 1, 3, 5, treatment response and outcome were collected. Treatment failure included death related to the initial infection, second source control treatment or a new onset intra-abdominal infection. The primary endpoint was to assess whether PCT thresholds (0.5 ng/ml or a drop from the peak of at least 80%) predict the initial treatment response.ResultsWe included 101 consecutive cases. Initial treatment failed in 36 patients with a subsequent mortality of 75%. Upon admission, PCT was doubled when treatment ultimately failed (21.7 ng/ml ± 38.7 vs. 41.7 ng/ml ± 75.7; P = 0.04). Although 95% of the patients in whom PCT dropped down below 0.5 ng/ml responded to treatment, 50% of the patients in whom PCT remained above 0.5 ng/ml also responded successfully to treatment. Moreover, despite a PCT drop of at least 80%, 40% of patients had treatment failure.ConclusionsIn perioperative intra-abdominal infections with shock, PCT decrease to 0.5 ng/ml lacked sensitivity to predict treatment response and its decrease of at least 80% from its peak failed to accurately predict treatment response. Studies in perioperative severe infections are needed before using PCT to tailor antibiotic use in this population.
Plant growth and production are greatly affected by water deficit worldwide and particularly in Tunisia. In this context, a study was conducted to analyse the response of three vetch species (Vicia narbonensis, V. sativa and V. villosa) to four water treatments: 100% (Control), 80%, 60% and 40% of field capacity. Water stress led to important changes of both leaf physiology and anatomy. V. narbonensis kept cells turgor by adjusting the osmotic potential without changes in cell walls elasticity. V. sativa showed the most pronounced reductions of leaf water potential and the lowest osmotic adjustment under water stress although cell walls elasticity has not changed. However, V. villosa kept a higher fraction of apoplastic water over V. sativa that allowed it to maintain cells turgor despite the rigidity of its cell walls. Drought did not affect lamina thickness but increased the palisade parenchyma at the expense of spongy parenchyma. It was characterised by leaf lamina increase and constant thickness of the spongy parenchyma: a strategy to cope with drought. Moreover, stomata were reduced in size but were increased in number in order to avoid water loss.
ABSTRACT:The secondary metabolite composition, antioxidant activities, and microbial inhibition properties of leaves of two Cistus species; C. monspeliensis and C. salvifolius were investigated using three solvent extracts (ethanol, hexane and distilled water). Ethanol extracts were most efficient at extracting phenolics, flavonoids and condensed tannins compared to hexane and distilled water for both Cistus species. A total antioxidant test (TAA) and two radical scavenging tests (DPPH and ABTS) indicated that the 70% ethanolic extract from C. salvifolius leaves had stronger antioxidant activity compared to the C. monspeliensis 70% ethanol extract, while the aqueous extract of C. monspeliensis was much stronger than the aqueous extract or the 70% ethanol extract of C. salvifolius. Overall, the polar extracts were more active in both species than the non-polar extracts. Thus aqueous ethanol extracts of the leaves of each Cistus species were tested for their ability to inhibit seven pathogenic microbial strains, including Escherichia coli ATCC 8739, Salmonella typhimurium NCTC 6017, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, Enterococcus faecalis, Aspergillus niger, and Candida albicans. Leaf ethanol extracts from both species were active against each microbial species, but the C. monspeliensis leaf ethanolic extract was much more active against several microbial species than that of C. salvifolius. For example, the C. salvifolius 70% ethanol extract showed its highest antimicrobial activity against P. aeruginosa and A. niger (MIC 3.1 mg/ml and MBC 6.3 mg/ml for both), while the C. monspeliensis extract showed much higher overall activity against E. coli, P. aeruginosa and C. albicana. (MIC 6.25 mg/ml and MBC 12.5 mg/ml for all three). In conclusion, maceration with 70% ethanol was the most efficient method for extracting total polyphenols, flavonoids, and condensed tannins from the two Cistus species, and the aqueous-ethanol extracts displayed the highest antioxidant and antimicrobial activities. Hence, the aqueousethanolic extracts of both species may be considered as potential sources of natural antioxidants and antimicrobial agents.
Although mean biases between the measurements were low, limits of agreement were too large to provide a clinically acceptable estimation of SvO(2) by ScvO(2) in these conditions. Variations in regional oxygen consumption seem to be the main factor worsening the relationship.
We aimed to examine the response of three tomato introgression lines (IL925.3, IL925.5 and IL925.6) to NaCl stress. These lines originated from a cross between M82 (Solanum lycopersicum) and the wild salttolerant tomato Solanum pennellii, each line containing a different fragment of the S.pennellii genome. Salt-sensitive phenotypes related to plant growth and physiology, and the response of antioxidants, pigments and antioxidant enzymes were measured. In general, salt stress decreased the fresh weight of leaves, leaf area and leaf number and an increase of Na + accumulation in aerial parts was observed, which caused a reduction in the absorption of K + and Ca 2+. Salt stress also induced a decrease in chlorophyll, carotenoids and lipid peroxidation (MDA) and an increase in anthocyanins and reduced ascorbate, although some differences were seen between the lines, for example for carotenoid levels. Guaiacol peroxidase, catalase and glutathione reductase activity enhanced in aerial parts of the lines, but again some differences were seen between the three lines. It is concluded that IL925.5 might be the most sensitive line to salt stress as its dry weight loss was the greatest in response to salt and this line showed the highest Na + ion accumulation in leaves.
Seeds of snail medic (Medicago scutellata L.) were assessed for their response to salt at the germination and seedling stages. NaCl at concentrations 86 and 170 mM decreased the final germination percentage. Embryonic axis length, water content and dry weight of embryonic axis and cotyledons were also reduced by salt treatment. Furthermore, 28-d-old plants were grown hydroponically with different NaCl concentrations (0, 86 and 170 mM). After 7 days of treatment, growth, water content and development of the different organs of M. scutellata plant were affected especially at the highest NaCl concentration (170 mM). However, NaCl did not affect root length and the number of stem shoots but reduced stem length and total leaf area. Salt treatment increased markedly the concentration of Na + in leaf and root tissues while reduced that of K + only in root and stem tissues. Lipid peroxidation revealed the damage of the membranes of roots and leaves. Moreover, showed a more intense suberization and lignification at the cambial zone of roots of M. scutellata, were observed under the effect of NaCl.
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