No abstract

Turkey rearing is getting popularity day by day in Bangladesh. Turkeypox is an important viral disease of turkey with great economic importance. In this study, turkeypox virus was isolated from a clinical case in Mymensingh, Bangladesh. Virus isolation was carried out through serial passage of viral inoculum into developing chicken embryo through chorioallantoic membrane (CAM) route. The virus isolate was confirmed by PCR targeting pox virus P4b gene. This is the first report of the isolation and PCR based molecular detection of turkeypox virus from a clinical case in Bangladesh.

The present study was carried out for the isolation and molecular detection of duck plague virus (DPV) for the development of inactivated vaccine seed from the local outbreaks. A total of 12 suspected dead duck samples were collected from commercial farms and local market at Sunamganj, Netrokona and Mymensingh districts. Then, the samples were processed and prepared inocula were inoculated into 9-12 days old duck embryonated eggs. In duck embryonated eggs, several passages (3-4) were performed before infection into DEF cell culture. Presence of viral DNA was confirmed by PCR using the primer for DNA polymerase gene. After PCR confirmation, virus cultured in DEF cell was used for the preparation of formalin (0.12%) inactivated and oil based adjuvanted vaccine and was experimentally injected to 18 ducklings and 5 were kept as control. TCID50 of the selected virus for vaccine preparation was 108.70/ml. The mean passive haemagglutination assay (PHA) titre of sera of samples at 0 days, 7 days and 14 days post vaccination were 4.0±0, 14.22±1.78 and 44.44±4.4, respectively, which indicated significant (p<0.01) increase of antibody titre. Embryonated duck eggs and DEF cell culture are effective for virus isolation and on the basis of PHA test, it could also be suggested that the experimentally developed DP vaccine can be used successfully for the prevention of DP in Bangladesh. Asian Australas. J. Biosci. Biotechnol. 2018, 3 (1), 78-85

Haemorrhagic septicaemia (HS) is an acute and highly fatal septicemic disease of cattle and buffaloes caused by Pasteurella multocida. The disease has a high morbidity and mortality in cattle, particularly in buffaloes. P. multocida are classified into several serotypes based on their capsular and somatic antigens. According to the capsular polysaccharides P. multocida according are classified into five serotypes designated A, B, D, E and F, while according to the cell wall lipopolysaccharides typing they are classified into 16 somatic serotypes (Cartet, GR., 1955; Carter and Alwis, 1989). The general and biochemical properties of the various strains are very similar, and from this point of view these organisms all belong to the single species, but different serotypes show different pathogenicity when tested in various hosts. The etiology of HS is P. multocida