Isolation of Pasteurella multocida from chickens, preparation of formalin killed fowl cholera vaccine, and determination of efficacy in experimental chickens

Akhtar, Mahmuda; Rahman, Md. Tanvir; Ara, Mosammat Shamim; Rahman, Mohammad Hafizur; Nazir, K. H. M. Nazmul Hussain; Ahmed, Sultan; Hossen, Md. Liakot; Rahman, Bahanur

doi:10.5455/javar.2016.c130

Cited by 8 publications

(13 citation statements)

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“…Also, these titers increased when the protection rate increased and decreased when the protection rate decreased as shown in tables 1 and 2. The same finding was obtained by Akhtar et al (2016) found that the antibody titer in birds of group A injected with 1ml of vaccine and group B injected with 0.5 ml of vaccine were 4.513 and 4.07 respectively at primary vaccination, and 4.893 and 4.37 respectively after booster vaccination indicating significant increase in the antibody titre as compared to the titre of the primary vaccinaterd birds. The same criteriawere obtained by Jabbri and Moazeni (2005) who stated that fowl cholera vaccine consisted of serotypes 1, 3 and 4 P. multocidastrains provided 70-100% protection against challenge with homologous strains.…”

Section: Discussionsupporting

confidence: 84%

“…On the other hand, Perelman et al (1990) recorded that ELISA test did not appear to be adequate for the evaluation of the degree of protection induced in turkey flocks where turkey poults vaccinated at one day old with inactivated P. multocida bacterin showed low titers of antibody as measured by ELISA but turkeys vaccinated at three and six weeks of age responded with a higher antibody titers and were resistant to virulent challenge with P. multocida. Akhtar et al (2016) vaccinated birds with formalin killed fowl cholera bacterin, determined antibody titers using ELISA and challenged birds with virulent strain of P. multocida then concluded that the prepared formalin killed fowl cholera vaccine induce protective immune response and conferred protection against challenge protection infection caused by the virulent P. multocida strains.…”

Section: Discussionmentioning

confidence: 99%

“…The chronic form of the disease can follow an acute stage or may be the only form of the disease present in the flock. Signs of this form generally linked to localized infection at wattles, sinuses, leg or wing joints, swollen eyes, twisted neck, rales and pin headed necrotic foci in the liver with a septicemic picture (Glisson et al, 2008;Akhtar et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Uses of Single Dose Dependent and Relative Potency Assays for Evaluation of Inactivated Fowl Cholera Vaccine

Salama¹,

Gadallah²,

Abo-Elkhir³

et al. 2019

jwpr

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Fowl cholera is a septicemic respiratory complex caused by Pasteurella multocida, widely distributed in poultry and other avian species and of major economic importance. A total of 37 different inactivated Pasteurella multocida vaccines from different sources either locally prepared or imported from different sources were comparatively tested for relative potency following both single dose and booster dose vaccination assays. The study objective was to minimize the time factor exhausted in the evaluation processes of the inactivated fowl cholera vaccines. So it is planned to compare between single and booster dose vaccinations and their related potency. Correlation between protection associated with the single dose and booster dose vaccination were evaluated and average requirement for protection was 43.7% in single dose vaccination assay compared to 76.2 % associated with booster dose vaccination assay. In the same concern, the correlation between both assays for the seroconversion was estimated using ELISA and the minimum requirement was 1.8× cut off value in the single dose vaccination assay compared to 2.25× cut off value in the booster dose vaccination assay. In conclusion, single dose vaccination assay could be valuable in the evaluation of inactivated fowl cholera vaccines through determination of protection indices and/or estimation of humoral immune response if the above mentioned data is considered.

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Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Uses of Single Dose Dependent and Relative Potency Assays for Evaluation of Inactivated Fowl Cholera Vaccine

Salama¹,

Gadallah²,

Abo-Elkhir³

et al. 2019

jwpr

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“…Conformation of the isolated organisms as P. multocida type B, the causal agent of haemorrhagic septicemia in buffaloes and cattle were done based on PCR as described by Townsend et al (1998), Panna et al (2015) and Akhtar et al (2016). Initially PCR was carried out to confirm the isolate as Pasteurella spp.…”

Section: Polymerase Chain Reaction (Pcr) For Pasteurella Multocida Tymentioning

confidence: 99%

Molecular detection of Pasteurella multocida Type B causing haemorrhagic septicemia in cattle and buffaloes of Bangladesh

et al. 2016

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Hemorrhagic septicemia (HS) is an acute septicemic disease that primarily affects cattle and buffaloes. The disease is caused by Pasteurella multocida sero types B:2 and E:2. The objective of this study was to isolate P. multocida from clinical cases and to confirm its identity using polymerase chain reaction (PCR) based approach. Clinical samples of two suspected cases of haemorrhagic septicemia of cattle and buffalo from Mymensingh and Rajshahi districts respectively were collected. Two isolates were isolated from these suspected cases and primarily identified as P. multocida based on morphological study, staining properties, and cultural and biochemical characteristics. The isolates were confirmed initially as P. multocida at genus level by PCR using genus specific primers. Later, the isolates were confirmed as P. multocida type B, the causal agent of haemorrhagic septicemia, by PCR with primers specific for P. multocida type B. These isolated organisms can be used as vaccine candidate for the production of effective vaccine against haemorrhagic septicemia.Progressive Agriculture 27 (2): 175-179, 2016

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“…Alum precipitated formalin killed FC vaccine is produced in the Livestock and Poultry Research and Production Centre (LPVRPC; formerly known as Poultry Biologics Unit) of the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh, while the oil-adjuvant FC vaccine is produced in Livestock Research Institute (LRI), Mohakhali, Dhaka, under the Department of Livestock Services (DLS), Government of People's Republic of Bangladesh (Bag et al, 2015). But, those which are imported or prepared from field cases of FC are much more expensive and there is a chance of being severe infection if sufficient measures are not taken (Akhtar et al, 2016). It is scientifically established that immune responses are influenced by various factors, such as breed and rearing region (Rana et al, 2010), age of the hosts (Dick and Avakian, 1991), and the isolate used as vaccine seed (Akhtar et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Immune Responses Induced in Chickens by Capsular Extract of P. Multocida Isolated From Farm Rat

Akter

Hossain

Hassan

et al. 2021

Res. Agric. Livest. Fish.

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An experiment was conducted to investigate the immune response induced in chickens by capsular extract of Pasteurella multocida isolated from rats wandering in and around the poultry farms. The rat isolate of P. multocida was isolated and identified by cultural, morphological, and biochemical characteristics, followed by capsular extract preparation and experimental vaccine development. The isolated P. multocida was found Gram-negative, non-motile, non-spore forming rod occurring singly or pains and occasionally as chains or filaments in Gram’s-staining method. The isolates consistently produced acid from dextrose, sucrose and mannitol but not fermented maltose or lactose. The Capsular antigen was extracted and confirmed by acriflavine test. Finally, experimental fowl cholera vaccine was prepared. Primary vaccination was performed at the dose rate of 5.6×107 CFU/ml through intramuscular and subcutaneous routes in birds of group A (10 birds) and group B (10 birds) and group C (10 birds) were control birds. Secondary vaccination was similarly performed after 15 days of primary vaccination in groups A and B. The levels of pre-vaccination and post-vaccination sera were determined by passive haemagglutination test. The passive haemagglutination antibody titre was recorded on 15 and 35 days of post vaccination in groups A and B. It was demonstrated that experimental capsular extract fowl cholera vaccine conferred 100% protection (p<0.01) against challenge infection and found to be safe. It could be suggested that after thorough field trial, the experimentally prepared capsular extract FC vaccine using rat isolate of P. multocida may be used side by side with conventional FC vaccine. Res. Agric., Livest. Fish.8(1): 117-124, April 2021

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Isolation of Pasteurella multocida from chickens, preparation of formalin killed fowl cholera vaccine, and determination of efficacy in experimental chickens

Cited by 8 publications

References 8 publications

Uses of Single Dose Dependent and Relative Potency Assays for Evaluation of Inactivated Fowl Cholera Vaccine

Uses of Single Dose Dependent and Relative Potency Assays for Evaluation of Inactivated Fowl Cholera Vaccine

Molecular detection of Pasteurella multocida Type B causing haemorrhagic septicemia in cattle and buffaloes of Bangladesh

Immune Responses Induced in Chickens by Capsular Extract of P. Multocida Isolated From Farm Rat

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