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Lectins are carbohydrate‐binding proteins that are highly selective for sugar groups on other molecules. Siglec5 is a cell‐surface lectin that belongs to the sialic acid‐binding Ig‐like lectins (Siglecs) and acts as a suppressor of immune responses. In this study, immunohistochemistry, western blotting and quantitative real‐time polymerase chain reaction (qRT‐PCR) were used to detect the expression of Siglec5 in the male reproductive tract of dromedary camels during the rutting season. Siglec5 displayed strong immunostaining in the cranial and caudal testicular regions and moderate immunostaining in the rete testis. Different parts of the epididymis showed varying immunoreactions to Siglec5. The spermatozoa in the testes and epididymis also showed positive immunostaining for Siglec5, whereas, the vas deferens showed negative immunostaining for the protein. The results obtained by western blotting confirmed the immunohistochemical detection of the protein in the testicular and epididymal tissues. The results of qRT‐PCR showed that Siglec mRNA was expressed differently in each part of the testis and epididymis; the highest levels of expression were observed in the caudal part of the testis and in the head of the epididymis. In conclusion, the present study revealed that Siglec5 is mainly located in the testis and epididymis, where sperm production and maturation occur. Therefore, this protein may play an essential role in the development, maturation and protection of camel sperm.

Administration of feed-based anticoccidials, as preventive measure, is one of the great successes in disease control for the ever-expanding poultry industry. The present study aimed at evaluating the efficacy of salinomycin medication against experimental infection with Eimeria tenella isolated from a local farm in Khartoum, Sudan. Forty-five one-day-old male broiler chicks (Ross 308) were randomized into 3 equal sets (control, infected, and medicated treatment groups). Infection was established by inoculating each of 36 birds with 1×10 5 sporulated oocysts when they were 24 days old. After 6 days, 9 birds of each group were bled for sera and sacrificed for post mortem examination. Birds of infected non-medicated group developed caecal lesions typical of E. tenella infection. Birds of salinomycin treatment at the concentration of 60 gm/ton of feed had highly diminished lesion scores (0.33) compared to infected non-medicated bird (3.00). Packed cell volume (PCV), triglycerides and cholesterol in birds medicated with salinomycin did not show marked difference as compared to broilers in negative control. There was no significant difference (P>0.05) in the levels of total protein and the concentration of calcium and phosphorus amongst experimental groups. It is concluded that salinomycin averted the negative impact of coccidiosis caused by E. tenella on broiler chicks; indicating that it could be recommended for establishing an efficient coccidian control programs under Sudan conditions.