Basement membranes in the kidney of the dromedary camel (Camelus dromedarius): An immunohistochemical and ultrastructural study

Eissa, Lemiaa; Ismail, Haider; Elhassan, Mortada Mahgoub Osman; Ali, H. A.

doi:10.1016/j.acthis.2019.03.005

Cited by 3 publications

(3 citation statements)

References 17 publications

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“…The collected specimens were ranged from 3 cm curved crown rump length CVRL about 73.7 days old foetus to 119 cm curved crown rump length CVRL about 390.6 days old foetus. The collected foeti were divided into three groups according to the method described and adopted by Eisa [7] as follows: i.…”

Section: Methodsmentioning

confidence: 99%

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Histogenesis of the Camel(Camelus dromedarius) Foetal Ovary with Special Emphasis to the Follicular Development and its Histometric Characteristics

Zolain,

Osman

2021

AJRAVS

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The aims of the present study are to investigate the histology of the different components of the foetal camel ovary, with special emphasis to the ovarian follicles and to determinate some histometric characteristics of the ovary during prenatal life. The present study was conducted in 79 foeti at different ages of development. The specimens were classified into first trimester (19 foeti), second trimester (26 foeti) and third trimester (34 foeti) according to the equation .The curved crown vertebral rump length (CVRL) equation Y= 0.366X-23.99 which was described by ELWishy et al., (1981) was used for the determination of the foetal age (X) in days from the known (Y) curved crown rump length in centimeters. Then the standard histological techniques were used to prepare the histological slides. The primordial germ cells migrated through the mesentery and were found between the cells of the surface epithelium. The first primordial follicles appeared at (25 cm CVRL) about (134 days old foetus) while at (28 cm CVRL) about (142 days old foetus) the first primary follicle was observed and at (65 cm CVRL) about (243 days old foetus) the first growing follicle was formed. The antral follicles were found only during the third trimester.

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Section: Methodsmentioning

confidence: 99%

“…The camelidae are among the most neglected and ignored mammals [2] although they play vital socioeconomic roles and support millions of people in the dry and arid zones of Asia and Africa [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Histogenesis of the Camel(Camelus dromedarius) Foetal Ovary with Special Emphasis to the Follicular Development and its Histometric Characteristics

Zolain,

Osman

2021

AJRAVS

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“…One of these mechanisms, which is the maintenance of electrolyte and water balance during dehydration and fast rehydration, is attributed to the kidney (Jararr and Faye, 2015). For a better understanding of this renal mechanism, several investigations have been carried out on the histology of the kidney in the dromedary camel (Abdalla and Abdalla, 1979;Safer et al, 1988;Eissa et al, 2018;Eissa et al, 2019, Abdalla, 2020. However, despite these studies, several histological features of the camel kidney remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Immunolocalization of intermediate filaments in the kidney of the dromedary camel (Camelus dromedarius)

Lemiaa¹,

Elhassan²,

Yaseen³

et al. 2022

EJA

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Intermediate filaments belong to a large family of proteins which contribute to the formation of the cytoskeleton. The immunolocalization of cytoskeletal proteins has been used extensively in the diagnosis of various renal pathologies. The present study described the immunolocalization of the cytoskeletal proteins vimentin, desmin, smooth muscle actin, and cytokeratin 19 in the normal kidney of the dromedary camel. Kidney samples from eight adult camels were processed for histology and immunohistochemistry. The kidney was enclosed in a renal capsule composed of vimentin immunoreactive fibroblasts and smooth muscle actin immunoreactive smooth muscle cells. The smooth muscle cells in the renal capsule did not exhibit desmin immunoreactivity. Podocytes forming the visceral layer of the glomerular capsule were immunoreactive for vimentin. Immunoreactivity for vimentin and smooth muscle actin in the parietal layer of the glomerular capsule varied, with both reactive and non-reactive cells observed. Intraglomerular mesangial cells were immunoreactive for smooth muscle actin and desmin, but non-reactive to vimentin. The endothelial lining of blood vessels was vimentin immunoreactive, while smooth muscle actin and desmin were demonstrated in the smooth muscle cells of the vessels. The thin limbs of the loops of Henle in cortical nephrons displayed vimentin immunoreactivity. The proximal and distal convoluted tubules, as well as the collecting ducts were negative to vimentin, smooth muscle actin, desmin and cytokeratin 19 immunostaining. In conclusion, the present study has revealed that similarities and differences exist in the immunolocalization of cytoskeletal proteins in the camel when compared to other mammals. The presence of smooth muscle actin in the parietal cells of the glomerular capsule suggests a contractile function of these cells. The results of the study indicate that vimentin and smooth muscle actin can be used as markers for the identification of podocytes and intraglomerular mesangial cells, respectively, in the camel kidney.

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Comparative physiological, morphological, histological, and AQP2 immunohistochemical analysis of the Arabian camels (Camelus dromedarius) and oxen kidney: Effects of adaptation to arid environments

et al. 2023

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Compared to other mammals, Arabian camels are ideal models for exploring the structural adaptations that enable camels to survive in arid environments. Thus, this study aimed to explore how evolutionary adaptation to arid conditions modifies the characteristics of the kidneys in Arabian camels (Camelus dromedarius) compared to oxen. Urine samples were physically and chemically analyzed. Harvested kidneys were subjected to topographical and fast spin echo magnetic resonance (FSE-MR) imaging. Histology, histomorphometry, and Aquaporin-2 (AQP2) expression by immunohistochemistry were also performed. Here, in dromedaries, sodium and potassium values in the urine were much higher (p=0.001, for both), whereas chloride was much lower (p=0.004) than the values of oxen. Compared with oxen, the level of the hormone aldosterone in serum was significantly lower (p=0.002), whereas creatinine and urea were significantly higher (p=0.005 and p=0.001, respectively). Uric acid in dromedaries and oxen did not differ significantly (p=0.349). Like sodium levels (p=0.001) in dromedary serum, chloride was also much higher (p=0.002) than in oxen. The average value of potassium was much lower (p=0.009) than that of oxen. Morphologically, anatomical and FSE MRI studies revealed that minor and major calyces were not found in dromedary kidneys. The renal pelvis was not found in oxen, and the major calyx was directly connected to the ureter. The dromedary kidney contained a wider medullary portion as well as increased diameters for renal corpuscles (RCs), proximal convoluted tubules (PCTs), and collecting tubules (CTs, p<0.05) compared with the oxen. We also noted that AQP2 was significantly expressed in dromedary nephron components, except for RCs, compared with oxen as shown by immunohistochemistry. Overall, these data strongly suggest that the dromedary has a greater ability to adapt to harsh desert conditions in terms of producing highly concentrated urine than oxen.

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Basement membranes in the kidney of the dromedary camel (Camelus dromedarius): An immunohistochemical and ultrastructural study

Cited by 3 publications

References 17 publications

Histogenesis of the Camel(Camelus dromedarius) Foetal Ovary with Special Emphasis to the Follicular Development and its Histometric Characteristics

Histogenesis of the Camel(Camelus dromedarius) Foetal Ovary with Special Emphasis to the Follicular Development and its Histometric Characteristics

Immunolocalization of intermediate filaments in the kidney of the dromedary camel (Camelus dromedarius)

Comparative physiological, morphological, histological, and AQP2 immunohistochemical analysis of the Arabian camels (Camelus dromedarius) and oxen kidney: Effects of adaptation to arid environments

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