Pancreatic secretion was studied in anaesthetized rats tested immediately after surgery or in conscious rats tested 48 h after the cannulation of the pancreatic duct. Pancreatic flow, protein output and enzyme output were measured over a 30-min period in the unstimulated state and after the intravenous injection of bolus doses of cholecystokinin-pancreozymin (CCK-PZ) ranging from 1·25 to 20 Crick-Harper-Raper units (CHRU). Each animal received three doses of CCK-PZ, as either ascending or descending doses.In anaesthetized rats there was a linear relationship between the log-dose of CCK-PZ and the flow, protein and enzyme output with both the ascending and descending doses. In contrast, in conscious rats flow was unaffected by CCK-PZ, and protein output was greatest after the first dose, whether this was given in the ascending or descending doses. At all CCK-PZ levels flow in anaesthetized rats was less than that seen in conscious animals, but at doses of CCK-PZ above 5·00 CHRU protein output was greater in anaesthetized rats than in conscious rats.Ultrastructural studies of the pancreas showed areas of focal cytoplasmic degeneration and possible blockage of the duct with cellular debris after administration of high doses of CCK-PZ to conscious rats. These changes may be responsible for the reduced protein output with the second and third dose of CCK-PZ in these animals. No such changes were seen in anaesthetized rats after similar doses of CCK-PZ.These studies show fundamental differences in the response of the pancreas to CCK-PZ in anaesthetized .and conscious rats. The mechanism for this difference is not clear, but it may represent a change in the normal response to CCK-PZ in the anaesthetized rats as a result of the effects of acute operative trauma, possibly acting through changes in pancreatic blood flow.Additional keyword: autophagosome
Summary. A lipid Jiiixture containing labelled oleic acid and labelled glyccrol-1-monoethcr was infused intraduodenally at a steady rate for 6 hours in unanaesthetized rats with l)iliary and thoracic dnct lymph fi.stulae. There were three groups, each of which received the same lipid mixture and dose but in dillerent physical states: miccllar in bile salts 10 mM, micellar in non-ionic detergent (Pluronic F68) and as a fine emulsion in bile salts 1 mM with no micellar phase. When a steady state of absorption had been reached the absorjition rate relati\e to area of small intestine utilized was greatest for micellar bile salt infusate and least for ni)n-niicellar enmlsion, with intermediate values for non-ionic micellar solution. There were differences in distdbiition of alxsorbed monoether label in the mucosa which suggested that metabolism of this model compound was affected by the rate of absorption. The percentage of monoether label which was saponifiable, i.e. which had been hydrolyzed and oxidized to fatty acid, was greater the more slowly the lipid was alxsorbed. On the other hand, the proportion of unsplit nionoethcr label which was estcrified wa.s smaller the more slowly the lipid was absorbed. .Since the results for non-ionic micellar infusate. containing no bilf salts, fell between those for high and low concentrations of bile salts, it seemed that metabolism of monoetber was uHected more by the absorptive Hnx rate than by a metabolic eftect of bile salts, per se.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.