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4 Egyptian mosquito species were tested for their ability to transmit the Egyptian ZH-501 strain of Rift Valley fever virus (RVFV) to golden Syrian hamsters. Culex (Cx.) antennatus was the most efficient vector, showing a 37.5% transmission rate following a hamster blood meal containing 10 suckling mouse intracerebral 50% lethal doses (SMILD50) per ml. Fully engorged mosquitoes of this species showed an infection rate of 85% with the mean viral titres of transmitting mosquitoes 100-fold higher than non-transmitters. Autogenous and anautogenous populations of Aedes (Ae.) caspius were tested separately, and the transmission rates were 23.1% and 9.7% respectively, following feeding on hamsters with similar levels of viraemia. Two anopheline species, Anopheles (An.) multicolor and An. pharoensis, showed 12.5% and 3.5% transmission rates under similar conditions. In these 3 species infection rates exceeded 75% and mosquitoes transmitting had a higher average titre than those not transmitting.

Cropping under conservation agriculture (CA) has become increasingly attractive among farmers in recent years. However, weed control may be more difficult during the transition to CA from conventional establishment methods due to the reduction in tillage intensity. Conversely, CA changes to weed dynamics can alter the weed seed bank in the longer run. In Bangladesh’s intensively cropped rice-based rotations, the nature of weed seed bank shifts over time after adopting CA are poorly known. Two 2-year studies were sampled from on-farm CA experiments under wheat-mungbean-winter rice and monsoon rice-mustard-winter rice rotations. We investigated the effects of reduced soil disruption in the form of strip-tillage (ST) combined with increased deposition of standing reside from previous crops (0 vs. 50%). The weed seed bank in 0–5, 5–10, and 10–15 cm depths of soil were quantified in a shade-house experiment by measuring weed emergence over 12 months in seedling trays. After 2 years of field study, the year-round count of emerged weeds from the seed bank showed that ST plus 50% mulch had a lower weed abundance and biomass and fewer weed species than that of conventional tillage (CT) without residue. The perennial weeds Ageratum conyzoides L., Alternanthera philoxeroides L., Cynodon dactylon L., Cyperus rotundus L., Jussia decurrence Walt., Leersia hexandra L., Scirpus mucronatus (L.) Palla., and Solanum torvum Sw. were enriched in the smaller-sized ST seed banks in terms of both density and biomass. The CT, on the other hand, was dominated by annual weeds: Cyperus difformis L., Cyanotis axillaris Roem., Echinochloa crus-galli (L.) Beauv., Eleusine indica L., Fimbristylis miliacea (L.) Vahl., and Rotala ramosior L. Overall, ST plus 50% residue had a more diverse seed bank than CT without mulch. The majority of weed seeds were amassed in the 0–5 cm soil depth of the ST, while most of them were accumulated in the 10–15 cm layer of the CT. The wheat-mungbean-winter rice rotation had a more diverse floristic composition with many more weed species than the monsoon rice-mustard-winter rice rotation.

As part of an evaluation of potential vectors of arboviruses during a Rift Valley fever (RVF) outbreak in the Nile Valley of Egypt in August 1993, we collected mosquitoes in villages with known RVF viral activity. Mosquitoes were sorted to species, pooled, and processed for virus isolation both by intracerebral inoculation into suckling mice and by inoculation into cell culture. A total of 33 virus isolates was made from 36,024 mosquitoes. Viruses were initially identified by indirect fluorescent antibody testing and consisted of 30 flaviviruses (all members of the Japanese encephalitis complex, most probably West Nile [WN] virus) and three alphaviruses (all members of western equine encephalitis complex, most probably Sindbis). The identity of selected viruses was confirmed by reverse transcriptase-polymerase chain reaction and sequencing. Culex antennatus (Becker) and Culex perexiguus Theobald accounted for five (17%) and 23 (77%) of the WN virus isolations, respectively. Despite isolation of viruses from 32 pools of mosquitoes (both WN and Sindbis viruses were isolated from a single pool), RVF virus was not isolated from these mosquitoes, even though most of them are known competent vectors collected during an ongoing RVF outbreak. Thus, it should be remembered, that even during a known arbovirus outbreak, other arboviruses may still be circulating and causing disease.

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Nitrogen and sulphur are fundamental macronutrients for the production of sunflower crop. Nitrogen is required consistently in larger amounts for sunflower production while common urea has more losses due to high solubility. On the other hand, sulphur application increases oil contents and availability of other essential nutrients (N, P, and K). Therefore, combined application of polymer coated urea with sulphur fertilization might be a promising option which can increase achene yield, oil contents, and nitrogen use efficiency (NUE). However, no particular studies have been conducted to explore the main and interactive effects of polymer coated urea and sulphur fertilization on growth, physiology, yield, oil contents, and NUE under arid field conditions. Hence, the current field experiment consisted of two nitrogen fertilizers [polymer coated urea (PCU) and common urea (CU)] and three sulphur fertilizer rates [S0 (0), S1 (30) and S2 (60) kg ha−1)] in a split-plot arrangement under randomized complete block design (RCBD) during spring season of 2019 and 2020. Experimental results revealed that growth, physiology, yield, oil contents, and NUE of sunflower crop were significantly improved with the application of nitrogen fertilizers, sulphur fertilizer rates, and their interaction. Meanwhile, NUE, achene yield and oil contents were increased by 16.0–17.2%, 16.5–17.0%, and 2.96–3.19% respectively with the application of PCU compared with CU. Furthermore, NUE, achene yield and oil contents were also increased by 12.8–13.3%, 13.1–13.7%, and 10.7–10.9%, respectively, due to sulphur fertilization of 60 kg ha−1compared with no sulphur application. Similarly, NUE, achene yield, and oil contents were increased by 32.9–39.5%, 31.7–32.6%, and 13.1–13.2% respectively with the application of PCU in combination with sulphur fertilization of 60 kg ha−1compared with CU × S0, which also evidenced a clear and positive interaction between nitrogen and sulphur. Conclusively, PCU (130 kg ha−1) in combination with sulphur fertilization of 60 kg ha−1 is promising option for obtaining higher achene yield, oil contents, and NUE for sunflower crop under arid environment, and hence, it might be a good agronomic adaptation strategy for sustainable production of sunflower.