Nematode traits after separate and simultaneous exposure to Polycyclic Aromatic Hydrocarbons (anthracene, pyrene and benzo[a]pyrene) in closed and open microcosms

Hedfi, Amor; Ali, Manel Ben; Hassan, Montaser M.; Albogami, Bander; Alzahrani, S.; Mahmoudi, Ebrahim; Karachle, Paraskevi K.; Rohal-Lupher, Melissa; Boufahja, Fehmi

doi:10.1016/j.envpol.2021.116759

Cited by 20 publications

(14 citation statements)

References 61 publications

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“…Each microcosm function as a closed system with a continuous ventilation provided by an aquarium pump. The reliability and validity of the used experimental methodology had been demonstrated in several published publications (Austen et al 1994;Schratzberger and Warwick 1998;Mahmoudi et al 2005;Ben Ali et al 2021;Essid et al 2021;Hedfi et al 2021). After 30 days, all of the experiments were terminated and the sediment from each microcosm was preserved in 4% formaldehyde buffered with hexamethylene tetramine to analyze the inhabiting meiobenthic nematodes (Essid et al 2020b;Wakkaf et al 2020).…”

Section: Contamination With Wastewatermentioning

confidence: 88%

“…Previous studies have demonstrated the sensitivity of nematodes to a variety of organic and mineral pollutants such as metals and polycyclic aromatic hydrocarbons (Mahmoudi et al 2005;Beyrem et al 2007;Mahmoudi et al 2007;Hermi et al 2009;Louati et al 2014;Ben Said et al 2015;Allouche et al 2020;Hedfi et al 2021;Ghosh and Mandal 2021). These pollutants are found also in urban and industrial wastewaters.…”

Section: Do Nematode Abundances and Taxonomic Diversity Affected By Wastewater?mentioning

confidence: 99%

“…Considering the previously published studies on the effects of wastewater spills on benthos (Keller 1983;Ansari et al 1984;Lambshead 1986), resulted that none of them has been devoted to free-living marine nematodes, despite the high potential of these invertebrates as bioindicators (Hedfi et al 2018(Hedfi et al , 2022. Indeed, several arguments exist to support nematode retention in environmental monitoring programs: (1) nematodes are the most abundant metazoans in marine sediments and provide statistically reliable results (Mahmoudi et al 2005), (2) their tiny sizes, short life cycles, and their high sensitivity to xenobiotics and environmental disturbances, makes that ideal for bioassays, furthermore they do not require large enclosures (Ben Ali et al 2021;Hedfi et al 2021).…”

Section: Introductionmentioning

confidence: 99%

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How effective is wastewater treatment? A case study under the light of taxonomic and feeding features of meiobenthic nematodes

Mahmoudi

Bejaoui

Ali

et al. 2021

Environ Sci Pollut Res

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A microcosm bioassay was designed to assess the efficacy of wastewater treatment methods used in sewage plants. The taxonomic and feeding characteristics of a meiobenthic nematode assemblage from Ghar El Melh lagoon (Tunisia) were used to achieve this goal. Nematode assemblages were exposed for 30 days to untreated wastewater (UW) obtained from the sewage treatment plant of Sidi Ahmed (Tunisia) and three mixtures with treated wastewater (TW33 = 33%, TW66 = 66%, and TW100 = 100%). Concerning the nematode abundance, the exposure to either treated wastewater (TW33-100) or untreated wastewater (UW) had no significant effect. In contrast, Shannon-Wiener index and Pielou's Evenness were clearly reduced by contamination with both types of wastewater, with a more pronounced negative impact prior to treatment in the sewage plant. The multivariate analyzes revealed a change in the taxonomic composition of the nematofauna in response to the contamination by untreated or treated wastewater. The relative abundances of species in wastewater replicates compared to controls reflected this taxonomic restructuring. Finally, Ascolaimus elongatus, Terschellingia communis, and Kraspedonema octogoniata were less represented in all experimentally treated units and could be considered as 'sensitive taxa to wastewater'. While, Paracomesoma dubium, appears to be a 'tolerant and/or opportunistic' species, showing increased relative abundances under all wastewater treatments.

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Section: Contamination With Wastewatermentioning

confidence: 88%

Section: Do Nematode Abundances and Taxonomic Diversity Affected By Wastewater?mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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How effective is wastewater treatment? A case study under the light of taxonomic and feeding features of meiobenthic nematodes

Mahmoudi

Bejaoui

Ali

et al. 2021

Environ Sci Pollut Res

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“…The study area is located on the west coast of the Bizerte bay, Tunisia (37°16'5.44"N, 9°53'4.88"E), about 500m from the wastewater rejection areas of the TRIC. The sediment was collected on April 8 th , 2015 (7 A.M) off the subtidal upper fringe, at a depth of 10 cm (Hedfi et al, 2021), using 10 cm 2 hand-cores (3.6 cm inner diameter) (Coull and Chandler, 1992) and subsequently transferred into a bucket. On the sampling day, two parameters were considered at the sediment-water interface, i.e pH and conductivity, and measured with a pH meter (WTW, model pH 330 / SET-1) and a temperature/conductivity meter (WTW LF 196,Weilheim,Germany), respectively.…”

Section: Sediment Samplingmentioning

confidence: 99%

Impact of treated sewage on meiobenthic nematodes: a case study from the Tunisian Refining Industries Company

et al. 2022

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The main objective of the current study was to assess the impact of the water taken from the ‘Tunisian Refining Industries Company’ on meiobenthic nematodes, before and after a series of treatments in decantation basins followed by its discharge in Bizerte bay, Tunisia. The comparison of environmental parameters of the two types of water was clearly indicative of an improvement in the quality of treated waters after a significant reduction in their loads in hydrocarbons. Overall, the water retained a good quality after being treated by ‘Tunisian Refining Industries Company’ before discharge in the sea. At the end of the experiment, differential responses were observed according to the richness of sediment in organic matter and hydrocarbons. Thus, it was apparent that the nematode assemblage exposed to the treated waters was closer to controls and associated to higher values of abundance, than that under untreated ones. It was also assumed that the species Microlaimus honestus De Man, 1922, Paramonohystera proteus Wieser, 1956 and Cyartonema germanicum Juario, 1972 are sensitive bioindicators of bad environmental statues and of hydrocarbon presence in the environment. On the other hand, Metoncholaimus pristiurus (Zur Strassen, 1894) Filipjev, 1918 would rather be classified as a positive bioindicative species of this type of pollutants.

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“…Benzo [a]pyrene (B[a]P), a kind of polycyclic aromatic hydrocarbons (PAHs), widely exists in the environment and comes from the incomplete combustion of petroleum, coal, wood, tobacco, and other carbon-containing compounds (Hedfi et al, 2021). Human expose to B[a]P mainly via inhalation of the polluted air and dust, digestion of contaminated food and water, and dermal contact (Yang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Alterations of DNA methylation andmRNAlevels ofCYP1A1,GSTP1,andGSTM1in human bronchial epithelial cells induced by benzo[a]pyrene

Liu

Hao

et al. 2022

Toxicol Ind Health

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Benzo[a]pyrene (B[a]P) is a known human carcinogen and plays a major function in the initiation of lung cancer at its first proximity. However, the underlying molecular mechanisms are less well understood. In this study, we investigated the impact of B[a]P treatment on the DNA methylation and mRNA levels of CYP1A1, GSTP1, and GSTM1 in human bronchial epithelial cells (16HBEs), and provide scientific evidence for the mechanism study on the carcinogenesis of B[a]P. We treated 16HBEs with DMSO or concentrations of B[a]P at 1, 2, and 5 mmol/L for 24 h, observed the morphological changes, determined the cell viability, DNA methylation, and mRNA levels of CYP1A1, GSTP1, and GSTM1. Compared to the DMSO controls, B[a]P treatment had significantly increased the neoplastic cell number and cell viability in 16HBEs at all three doses (1, 2, and 5 mmol/L), and had significantly reduced the CYP1A1 and GSTP1 DNA promoter methylation levels. Following B[a]P treatment, the GSTM1 promoter methylation level in 16HBEs was profoundly reduced at low dose group compared to the DMSO controls, yet it was significantly increased at both middle and high dose groups. The mRNA levels of CYP1A1, GSTP1, and GSTM1 were significantly decreased in 16HBEs following B[a]P treatment at all three doses. The findings demonstrate that B[a]P promoted cell proliferation in 16HBEs, which was possibly related to the altered DNA methylations and the inhibited mRNA levels in CYP1A1, GSTP1, and GSTM1.

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Nematode traits after separate and simultaneous exposure to Polycyclic Aromatic Hydrocarbons (anthracene, pyrene and benzo[a]pyrene) in closed and open microcosms

Cited by 20 publications

References 61 publications

How effective is wastewater treatment? A case study under the light of taxonomic and feeding features of meiobenthic nematodes

How effective is wastewater treatment? A case study under the light of taxonomic and feeding features of meiobenthic nematodes

Impact of treated sewage on meiobenthic nematodes: a case study from the Tunisian Refining Industries Company

Alterations of DNA methylation andmRNAlevels ofCYP1A1,GSTP1,andGSTM1in human bronchial epithelial cells induced by benzo[a]pyrene

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