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The aim of the current research was to present a methodological approach allowing reproducible morphometric and morphological (Chiu/Park scale) analyses of the alterations in the intestines of broilers exposed to heat stress. Ross broilers were exposed over four consecutive days to a high-temperature regime in controlled climate rooms, with a day temperature of 39°C (±1°C) and a night temperature of 25°C (±1°C), respectively. A control group was kept at an ambient temperature of 25°C (±1°C) during the entire experimental period. At the end of the exposure period, the birds were sacrificed and specimens were taken of the duodenum, jejunum and ileum for histology. Blood was collected for oxidative stress analysis. Histo-morphological and morphometric analyses of the intestines indicated that the duodenum and jejunum showed more damage than the ileum. The major alterations in the control intestines were limited to the villus tips, while heat stress led to villus denudation and crypt damage. When compared with morphologically normal villi, heat-stress-associated alterations were also observed in villus height (decreased), villus breadth at base (increased) and epithelial cell area (decreased). Birds exposed to heat stress presented with an increase in glutathione peroxidase activity and a decreased antioxidant capacity. It can be concluded that the chosen model allows a reproducible quantification of heat stress effects, which is suitable for the evaluation of dietary intervention strategies to combat heat stress conditions.

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