Objectives A previous study reported that the hexane fraction (HF) obtained from Pereskia aculeata leaves showed promising topical anti-inflammatory activity. Intending its future use in clinical practice, a herbal medicine cream named INFLATIV was developed. Its anti-inflammatory and antipsoriatic potential were investigated. INFLATIV was subjected to preliminary accelerated stability tests and to a degradation profile assessment. Methods INFLATIV was prepared at 6% and 12%. The anti-inflammatory activity was assessed by croton oil single and multiple application challenge in mice. Mouse tail test was used for antipsoriatic potential investigation. Cutaneous atrophy test was performed. Preliminary accelerated stability tests were performed together with a degradation profile by GC-MS analysis. Key findings The anti-inflammatory activity shown by INFLATIV was comparable to dexamethasone. However, the skin atrophy caused by that drug was not observed. INFLATIV modified skin parakeratotic differentiation into orthokeratosis, which revealed its antipsoriatic potential. The ingredients used were suitable to carry the bioactives as they were well permeated by the skin. The preliminary accelerated stability tests indicated that INFLATIV 6% is more stable than 12%. Conclusions The results demonstrated the relevant therapeutic and marketing potentials of INFLATIV, which is likely to be further evaluated in clinical trials for drug registration process with regulatory agencies.
Because it promotes the lightening of pigment spots, tyrosinase inhibition is one of the mechanisms of depigmenting cosmetic products. Considering the adverse effects produced by synthetic depigmenting actives, the search for new therapeutic options is desirable, and plant extracts are possible candidates for hyperpigmentation treatment. Glycolic extracts of Cecropia pachystachya Trécul are, therefore, the focus of this study. Its chemical characterization, antioxidant activity, tyrosinase inhibition, and cell viability were evaluated. Glycolic extracts were obtained by macerating the leaves of C. pachystachya in grain alcohol and glycerin or propylene glycol. Both had a similar chemical constitution, the glycerin being more efficient in concentrating phenolic compounds and flavonoids. Analyses by UHPLC-MS detected quinic acid, chlorogenic acid isomers, proanthocyanidin dimers type B and C, catechin/epicatechin, orientin/isoorientin, isoorientin 2"-O-xyloside, vitexin/isovitexin, and rutin. 5-O-caffeoylquinic acid was then quantified was then quantified, with predominance in the extract produced with propylene glycol. These extracts showed a high antioxidant capacity by the method of DPPH, β-carotene, and nitric oxide. As for depigmenting activity, both extracts were able to inhibit tyrosinase. Cell viability assay also revealed that the extracts could safely be used in concentrations of ≤ 125 µg/mL. Thus, this study demonstrated for the first time that the glycolic extracts of C. pachystachya have promising chemical and biological characteristics for the development of a multifunctional cosmetic with antioxidant and tyrosinase-inhibition activities.
Pereskia aculeata Miller é uma trepadeira arbustiva popularmente conhecida como "orapro-nobis". Na medicina tradicional, as folhas são utilizadas como anti-inflamatórias, cicatrizantes e emolientes. O objetivo desse estudo foi investigar a constituição química e o potencial antioxidante de extratos glicólicos de P. aculeata. Durante dez dias, as folhas foram maceradas à temperatura ambiente com glicerina e álcool de cereais, sendo obtidos os extratos PA01 e PA02. Ao final do processo, o extrato PA01 foi aquecido a 50 °C por 30 minutos. Esta foi a primeira vez em que extratos de P. aculeata foram obtidos com os referidos solventes extratores. O teor de compostos fenólicos foi avaliado utilizando-se o reagente de Folin-Ciocalteau e o potencial antioxidante foi determinado pelo método de sequestro do radical DPPH. PA01 e PA02 apresentaram um teor de fenóis totais de 13,84 ± 4,29 e 18,35 ± 2,12 mg em equivalentes de ácido tânico/g de extrato, respectivamente. No método do DPPH, os valores de CI50 encontrados foram de 11,93 ± 1,84 e 9,91 ± 2,45 µg/mL, respectivamente, demonstrando uma boa atividade antioxidante. Além disso, o aumento da temperatura não foi um fator decisivo na extração, visto que o conteúdo fenólico e o potencial antioxidante foram estatisticamente semelhantes para ambos os extratos. O potencial antioxidante pode ser atribuído, pelo menos em parte, ao conteúdo fenólico, visto que PA01 e PA02 foram capazes de extrair esses compostos de forma semelhante. Portanto, os extratos glicólicos de P. aculeata apresentam potencial para contribuir com a prevenção de diversos distúrbios associados com a produção de radicais livres.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.