Apeiba tibourbou is a common species found in the rainforests of South America. The aim of the present work is to determine the most favorable condition for micropropagation. Seeds were submitted to both thermal and mechanical scarification, sterilized and inoculated in test tubes with ½ MS, with or without the addition of sucrose. The explants were divided in a factorial experiment of four concentrations (0; 0.1; 0.5 and 1.0 mgL -1 ) of 6-benzylaminopurine (BAP) and three auxins at the concentration of 0.01 mg.L -1 : indole-3-acetic acid (AIA), indole-3-butyric acid (AIB) or naphthalene-acetic acid (ANA). Regarding the production of new sprouts, auxins had an inhibitory effect on sprouting, and the best results were obtained with BAP at the concentration of 0.5 mgL -1 without auxins or with AIA. However, regardless the culture medium the sprouts died in the following subculture. All the explants cultivated in the absence of growth regulators rooted. All combinations of growth regulators inhibited the formation of roots, except in the presence of AIA alone. The multiplication using sprouts formed in vitro remain a challenge to overcome.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.