Two populations of Palmer amaranth from New Mexico have been confirmed to be resistant to glyphosate. In the present study, the molecular basis of resistance and the mode of inheritance of resistance in those populations were investigated. Quantitative real-time polymerase chain reaction analysis indicated up to an eightfold increase in genomicEPSPScopy number in glyphosate resistant plants compared with susceptible plants. The relative genomicEPSPScopy number of resistant plants was positively correlated with the relativeEPSPScDNA expression levels. Eight hours after treatment with glyphosate, the shikimate accumulation levels in resistant plants were negatively correlated with the genomicEPSPScopy numbers. Multiple sequencing of theEPSPScDNA of resistant plants did not reveal any glyphosate resistance-conferring mutations. The evaluation of F1, reciprocal F1, and F2Palmer amaranth families indicated that resistance to glyphosate does not follow a single-gene segregation pattern. Results suggest that theEPSPSamplification is the primary molecular basis of resistance in glyphosate resistant populations of Palmer amaranth from New Mexico.

Greenhouse experiments were conducted at Wooster, OH, during 2010 and 2011 growing seasons to evaluate the responses of five wine grape cultivars to sublethal doses of 2,4-D, dicamba, and glyphosate, and the 'Riesling' grape to mixtures of 2,4-D plus glyphosate and dicamba plus glyphosate. Treatments were made using a spray system calibrated to deliver 0.757 L min À1 at 276 kPa and 4.8 km h À1. Herbicides were delivered through 8002 flat spray nozzles and applied at 1/30, 1/100, and 1/300 of the recommended field rate of 840, 560, and 840 g ae ha À1 for 2,4-D, dicamba, and glyphosate, respectively. Injury was observed in all treatments 7 d after treatment (DAT). However, injury symptoms greater than 10% were observed 42 DAT in plants treated with 2,4-D at all rates and plants treated with dicamba at the two highest rates. Injury (35%) at 357 DAT was noted only in plants treated with the highest rate of 2,4-D. French hybrids showed slightly less injury symptoms compared with wine grapes at 7 and 42 DAT. Shoot length reduction in plants treated with 2,4-D at the highest rate was 43, 84, and 16% at 7, 42, and 357 DAT, respectively. Glyphosate caused the fewest injury symptoms in Riesling compared with 2,4-D and dicamba when applied separately or tank mixed with glyphosate. Shoot length reduction in Riesling was observed 42 DAT with all rates of 2,4-D, with and without glyphosate and dicamba, and dicamba plus glyphosate at the highest rate; however, at 357 DAT, no effect was observed in shoot length. Spray drift of 2,4-D and dicamba can severely injure grapes, with injury increasing with increased exposure. The combination of 2,4-D plus glyphosate caused greater injury and shoot length reduction in grapes than glyphosate applied alone. Nomenclature: 2,4-D; dicamba; glyphosate; wine grape, Vitis vinifera L. 'Riesling', 'Chardonnay', 'Chardonel', 'Traminette', 'Vidal blanc'. Key words: Crop injury, herbicide drift.Experimentos de invernadero fueron realizados en Wooster, Ohio, durante las temporadas de crecimiento 2010 y 2011, para evaluar la respuesta de cinco cultivares de uva para vino a dosis subletales de 2,4-D, dicamba, y glyphosate, y de la uva 'Riesling' a mezclas de 2,4-D más glyphosate y dicamba más glyphosate. Los tratamientos fueron realizados usando un sistema de aspersión calibrado para liberar 0.757 L min À1 a 276 kPa y 4.8 km h À1 . Los herbicidas fueron asperjados mediante una boquilla de abanico plana 8002 a 1/30, 1/100, y 1/300 de las dosis recomendadas de campo de 840, 560, y 840 g ae ha À1 para 2,4-D, dicamba, y glyphosate, respectivamente. Se observó daño en todos los tratamientos 7 d después del tratamiento (DAT). Sin embargo, los síntomas de daño superiores a 10% fueron observados 42 DAT en plantas tratadas con 2,4-D con todas las dosis y plantas tratadas con dicamba con las dos dosis más altas. El daño (35%) 357 DAT se notó solamente en plantas tratadas con la dosis más alta de 2,4-D. Los híbridos franceses mostraron síntomas de daño ligeramente menores al compararse con uvas para vino a 7 y 4...

Two populations of Palmer amaranth suspected of being resistant to glyphosate have been reported since 2007 in pecan orchards in Doña Ana County, New Mexico. The objectives of the study were to confirm and evaluate the level of resistance, to evaluate the effectiveness of alternative herbicide mechanisms of action, and to compare the cost of effective alternative herbicides for weed management in pecan orchards. Greenhouse experiments indicated that the resistant populations were able to survive glyphosate at 736 g ae ha−1. Compared with a susceptible (S) population, one of the resistant (R) populations had sevenfold resistance to glyphosate. POST application of 12 herbicides, with five different mechanisms of action, all provided at least 88% control of both R and S populations when applied at their recommended field rates. PRE application of trifluralin and metolachlor also provided more than 99% control of R and S populations. The results of field studies indicated that the financial benefit of season-long weed management with glyphosate, in pecan orchards, was comparable with some of the tested alternative herbicides.

Iris yellow spot virus (IYSV) causes a foliar disease in onion (Allium cepa L.) that results in a reduction in bulb size. Currently, no IYSV-tolerant or -resistant cultivar exists and a genetic source for tolerance or resistance has not been identified. Because other disease control methods are limited, host plant resistance offers the best hope to combat this disease. In this study, 13 winter-sown onion entries were screened for iris yellow spot (IYS) symptoms during the 2007 and 2008 cropping seasons. Twenty plants from each plot were observed and rated weekly during the growing seasons for straw-colored, necrotic lesions, typical of IYSV infection. Collected plant samples were assayed for IYSV by enzyme-linked immunosorbent assay (ELISA) and reverse-transcription polymerase chain reaction (RT-PCR). Visual rating was done using a scale of 1 to 9 with 1 representing no symptomatic tissue and 9 representing more than 50% tissue damage. Two different plant sampling methods were used in disease rating to determine their effect on mean severity and to correlate disease severity with absorbance values. Of the entries tested, plants of NMSU 05-33-1 exhibited a delay in symptom expression and lower IYSV levels relative to plants of other entries. Plants of ‘Denali’ and ‘Gelma’ appeared to be more susceptible to IYSV than plants of other entries. Plant selection within the plot over time did not influence disease rating values. When the same plants were rated and sampled for IYSV using ELISA, there was a strong, positive correlation between rating and absorbance values.

Field experiments were conducted at Wooster, OH, in 2010 and 2011 to evaluate the effect of simulated drift rates of 2,4-D, dicamba, and 2,4-D plus glyphosate on processing broccoli and bell pepper. Treatments were made in July of each year when bell pepper and broccoli were at the 10- and eight-leaf stage, respectively, and included five 2,4-D rates (1/50, 1/100, 1/150, 1/200, and 1/400 of the recommended field rate of 840 g ae ha−1), five dicamba rates (1/50, 1/100, 1/150, 1/200, and 1/400 of the recommended field rate of 560 g ae ha−1), and three rates of 2,4-D plus glyphosate (1/100, 1/200, and 1/400 of the recommended field rates). Crop injury was recorded at 7 and 28 d after treatment (DAT). Broccoli and bell pepper responded differently to simulated drift rates each year with higher initial injury ratings observed in 2010, and more persistent symptoms in 2011. 2,4-D at the 1/50 rate reduced broccoli yield by approximately 50% in 2010. Simulated drift rates of 2,4-D did not cause broccoli yield reduction in 2011, nor did simulated drift rates of dicamba, or 2,4-D plus glyphosate reduce yield either year. Although simulated drift treatments did not reduce total yield of bell pepper, the timing of fruit maturity was affected. Yield at first harvest was reduced by high simulated drift rates of each herbicide and by the herbicide tank mix. These results indicate that broccoli and bell pepper are sensitive to very low doses of 2,4-D and dicamba that are typical of those encountered in drift events. The impact of actual drift on delayed maturity and total yield of these crops is likely to be costly for the farmer.