BackgroundStudies on selected metabolites profiling of Orthosiphon stamineus extracts using chromatographic and spectroscopic techniques combined with chemometric tools have not been fully elucidated. Thus present study was performed to profile selected metabolites in O. stamineus leaves extracts using HPLC and FTIR combined with chemometric tools and correlated with biological activities.MethodsFive different extracts were prepared using three methods; maceration, soxhlet and reflux. The extracts were analyzed using UV-Vis, HPLC and FTIR techniques. Analysis of selected primary and secondary metabolites was also evaluated. The antioxidant and cytotoxic activities of the extracts were evaluated. Chemometric tools were employed to classify the extracts based on HPLC analysis and FTIR fingerprints.ResultsThe ethanolic extract using maceration characterized high content of phenolics and flavonoids, (rosmarinic acid and eupatorin) with high antioxidant activity. Ethanolic (50 %) and methanolic extracts using soxhlet showed high proteins and glycosaponins. Water extracts using reflux and maceration showed high polysaccharides. Methanolic extract (50 %) using soxhlet and methanolic extract using maceration showed strong cytotoxic effect against MCF7 and HCT116 cell lines, respectively. Antioxidant and cytotoxic activities showed significant correlation with selected primary and secondary metabolites. HPLC fingerprints combined with chemometrics showed the extracts have been clustered based on selected major peaks profile. FTIR fingerprints combined with chemometrics showed that the extracts have been clustered based on protein and polysaccharide contents.ConclusionTen different extracts of O. stamineus have showed significant differences in the content of selected primary and secondary metabolites as well as the biological activities. Chemometric tools were able to classify and discriminate the distinctive features of extracts thus can be correlated with the biological activities.
Background:Orthosiphon stamineus Benth. (Lamiaceae) is a traditional medicinal plant which has been used in treating various ailments such as kidney diseases, bladder inflammation, arthritis and diabetes. The leaves contain high concentration of phenolic compounds, thus, rosmarinic acid (RA), 3’-hydroxy-5, 6, 7, 4’-tetramethoxyflavone (TMF), sinensetin (SIN) and eupatorin (EUP) were chosen as a marker compounds for standardization of various O. stamineus leaf extracts.Objective:The aim was to develop and validate a new high-performance liquid chromatography (HPLC) method for quantification of 4 marker compounds (RA, TMF, SIN, EUP) in various O. stamineus leaf extracts.Materials and Methods:The method was developed and validated using RP-HPLC-diode-array detection at 320 nm for accuracy, precision and limits of detection and was applied for quantification of it markers in five different extracts prepared in solvents with increasing polarity, using a gradient mobile phase 0.1% formic acid: Acetonitrile at a flow rate of 1 ml/min on reverse phase acclaim polar advantage II C18 column (3 μm, 3 × 150 mm) with 18 min separation time.Results:The developed method provided satisfactory precision, and the accuracy of this method was in the range of 90.2% to 105.5%. All of 4 compounds showed good linearity at R2 > 0.999.Conclusion:The developed method is a simple, cost effective with shorter run time (18 min) in comparison to previous methods (30 min) and utilization of environmental-friendly solvents system. Therefore, this method has the potential to replace currently used methods in the routine standardization work of O. stamineus extracts, raw materials and its commercial products.
Purpose: To investigate the toxicity of Labisia pumila standardized extract (LPE) and its liposomal extract (LLP). Methods: For acute toxicity study, LPE or LLP was orally administered (2000 mg/kg) in single doses to Sprague Dawley rats and the routine activity of the rats was continuously monitored for a total of 14 days. After 14 days of treatment, all rats were sacrificed and their vital organs were excised, weighed and macroscopically examined, while for a repeated dose toxicity study, the rats were orally administered with LPE or LLP at the selected doses (250, 500 and 1000 mg/kg) for a period of 28 days. The animals were sacrificed (anaesthetized by sodium pentobarbitone and blood was collected by cardiac puncture), followed by examination of their body organs and blood serum. Results: LPE and LLP at 2000 mg/kg did not produce mortality or significant changes in the general behaviour, body weight and organ gross appearance of the rats. In repeated dose toxicity study no significant changes in, growth, organ weights, haematological parameters, biochemical values and histological features of vital organs of the treated groups, compared to the control group. Conclusion: The no-adverse-effect-level for LPE and LLP is (1000 mg/kg/day) when administered orally for 28 days.
This study evaluates the primary and secondary metabolite profiles of Jack (EL) stems and leaves to the roots. A total of six types of extracts were tested. The extracts showed high content of glycosaponins, polysaccharides, proteins and phenolics. The presence of flavonoids and phospholipids was also detected. High Performance Thin Layer Chromatography (HPTLC) and High Performance Liquid Chromatography (HPLC) analysis showed the presence of bioactive marker of EL root, eurycomanone and 14,15ß leaf extracts. Primary and secondary metabolites identified were reported to associate with the enhancement of ergogenic and aphrodisiac activities in animal and human subjects. shows that stem and leaves of
Coconut sap from Cocos nucifera could be obtained from the coconut flower, and it is quite a popular fresh drink in Malaysia. Due to microbial activity, coconut sap may change to alcoholic and acidic by spontaneous fermentation. Traditionally, chengal wood chips (Neobalanocarpus heimii) have been added in coconut sap to slow down the fermentation process. This research aims to evaluate the physicochemical properties (pH, total acidity, total soluble solids, colour), volatile organic compounds (VOCs) and antimicrobial properties of coconut sap with and without the presence of chengal wood chips for ten weeks at 4 °C and 25 °C storage temperatures. The pH value of both samples was between 3.60 ± 0.01 to 6.66 ± 0.04. Total acidity ranged from 0.29 ± 0.11% to 4.26 ± 0.25%. Total soluble solids in both samples were around 8.3 ± 0.05 to 17.3 ± 0.05 °Brix. Colour analysis was also carried out based on the lightness value. Eighteen of VOCs were found in coconut sap with chengal wood chips versus 20 compounds without the chengal wood chips. Coconut sap with chengal wood chips kept at 4 °C had the lowest total plate count (1.19 x 104 - 1.78x106 CFU/mL) at week one until week seven, while total plate count of coconut sap without chengal wood chips was recorded as too numerous to count (TNTC) throughout the weeks. Chengal wood chip extracts inhibited the growth of Staphylococcus aureus, Escherichia coli, Candida albicans, and Aspergillus brasiliensis, as shown by a clear zone of inhibition. In conclusion, the presence of chengal wood in coconut sap has helped to slow down the fermentation process and inhibit the growth of microorganisms. Hence it can help to prolong the shelf life of coconut sap.
In Malaysia, commercial stingless bee honey is in demand due to its nutritional and healing properties, especially in the cosmetic, food and beverage and pharmaceutical industry. However, up to date, these honey products from various districts of Kelantan were not subjected to compliance according to the specification of the Malaysian Standard (MS) 2683:2017. Thus, the objective of the current study was to determine the compliance of selected stingless bee honey (SBH) in Kelantan based upon physicochemical properties and microbiological analysis following MS 2683:2017 specifications. Physicochemical analysis of commercial stingless be honey shows the value of moisture, ash, hydroxymethylfurfural (HMF) and pH were in the range of 27.05 ± 1.39 to 32.61 ± 2.79 %, 0.08 ± 0.01 to 0.14 ± 0.01 g, 8.78 ± 0.92 to 218.66 ± 0.70 mg/kg and 2.34 ± 0.01 to 3.22 ± 0.02, respectively. There was an absence of total coliform in all samples. In summary, all samples of stingless bee honey complied with MS 2683:2017 specification for physicochemical properties and microbial contaminant limits of total plate count (TPC) and total coliform. However, for the microbial contaminant limit of yeast and mold, only sample 6 was contaminated.
Syzygium myrtifolium Walp. (Syzygium campanulatum Korth), Myrtaceae, is locally known as "Kelat paya" in Malaysia. Traditionally, it is used as a remedy for stomach pain. The goal of the present study was to investigate the toxicological potential as well as the antidiarrhoeal and antispasmodic activities of betulinic acid, dimethyl cardamonin and standardized non-formulated and nano-formulated ethanol and supercritical fluid extracts prepared from leaves of S. myrtifolium. The standardized extracts did not produce in vivo toxicity. Both the compounds and standardized extracts showed dose-dependent antidiarrhoeal activity by examining changes in the percentage of liquid stools and percentage of defecation frequency. Dimethyl cardamonin and standardized extracts showed antispasmodic activity on the isolated ileum of guinea pigs. Compared with hyoscine-N-butylbromide, dimethyl cardamonin and standardized extracts produced significant, potent antagonizing activity in ileum contractions induced with acetylcholine. Furthermore, the antagonistic potential of S. myrtifolium active markers against muscarinic type M2 and M3 receptors was investigated by molecular docking.
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