Simultaneous Determination of Two Isomers of Asarone in &lt;i&gt;Piper sarmentosum&lt;/i&gt; Roxburgh (Piperaceae) Extracts using Different Chromatographic Columns

Hamil, Mohd Shahrul Ridzuan; Memon, Abdul Hakeem; Majid, Amin Malik Shah Abdul; Ismail, Zhari

doi:10.4314/tjpr.v15i1.22

Cited by 3 publications

(5 citation statements)

References 19 publications

(15 reference statements)

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“…Asarone is a volatile compound that belongs to the phenylpropanoid group. The compounds showed favourable solubility in non-polar solvent compared to solvent at a higher polarity (Hamil et al, 2016). From the preliminary screening, we found that SC-CO 2 extraction at a lower pressure was the most effective method to maximise recovery of asarone; thus, further optimised using the response surface model.…”

Section: Extraction Yield Of Asarone Using Sc-co 2 and Conventional S...mentioning

confidence: 99%

“…Quantification of asarone in the P. sarmentosum extracts was performed using HPLC described by Hamil et al (2016). Briefly, the analysis was performed on Agilent Technologies 1260 Infinity HPLC system (USA).…”

Section: Hplc Analysismentioning

confidence: 99%

“…Several studies have reported that α-and β-asarone possess psychoactive, carcinogenic, genotoxic, and cytotoxic properties. Their presence was reported in Acorus calamus, Acorus gramineus, Asarum europaeum, Guatteria gaumeri, and Piper sarmentosum up to 95% in various extracts and essential oils (Authority, 2009;Hamil et al, 2016). The administration of products with a high level of asarone could pose health issues among consumers with observed side effects such as tachycardia, dizziness, tremor, irregular breathing, pallor, anxiety, nausea, and vomiting.…”

Section: Hplc Analysis Of Sc-co 2 Residuementioning

confidence: 99%

“…In a previous study (Hamil et al, 2016), α-and β-asarone were reported to be present in various amounts in alcohol and hydroalcoholic extracts of P. sarmentosum, thus, limiting the utilisation of the extracts for food and nutraceutical products. The present work aims to optimise the extraction of α-, β-, and ɣ-asarone from P. sarmentosum leaves using SC-CO 2 technology and compare the asarone level of the leaves extracted with conventional solvents prior to and after SC-CO 2 treatment.…”

Section: Introductionmentioning

confidence: 98%

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Optimisation of asarone removal from Piper sarmentosum Roxburgh leaves using supercritical carbon dioxide extraction: the Box-Behnken design

Hamil

Mohamed

Ismail

2022

Braz. J. Pharm. Sci.

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Piper sarmentosum is a herbaceous shrub with numerous pharmacological benefits. However, the presence of two toxic phenylpropanoids (α-and β-asarone) limits the medicinal usage of the plant. In this study, the extraction of three asarone isomers, namely α-, β-, and ɣ-asarone was optimised using supercritical carbon dioxide extraction (SC-CO 2 ) combined with Box-Behnken experimental design. Comparison of asarone contents in different conventional solvent extracts of P. sarmentosum leaves prior to and after SC-CO 2 extraction was performed. The SC-CO 2 method successfully maximised the extraction of α-, β-, and ɣ-asarone at P = 81.16 bar, T = 50.11°C, and t = 80.90 min, yielding 13.91% α-asarone, 3.43% β-asarone, and 14.95% ɣ-asarone. The SC-CO 2 residue of the leaves re-extracted with conventional solvents showed a significant decrease of asarone ranging from 45% to 100% (p<0.001) compared to their counterparts without SC-CO 2 treatment. α-, β-, and ɣ-asarone were completely removed in the ethanol extract of the residue. These findings suggested that the optimised SC-CO 2 extraction parameters may serve as a quick treatment step for the selective removal of asarone from P. sarmentosum to develop safer extracts for the food and nutraceutical industries applications.

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Section: Extraction Yield Of Asarone Using Sc-co 2 and Conventional S...mentioning

confidence: 99%

Section: Hplc Analysismentioning

confidence: 99%

Section: Hplc Analysis Of Sc-co 2 Residuementioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Optimisation of asarone removal from Piper sarmentosum Roxburgh leaves using supercritical carbon dioxide extraction: the Box-Behnken design

Hamil

Mohamed

Ismail

2022

Braz. J. Pharm. Sci.

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“…A gradient HPLC system for the separation of vitexin and naringenin was performed based on the validated method described by Hamil et al (2016) using an Agilent 1100 HPLC system and ZORBAX Eclipse Plus C-18 column (Agilent, USA). The mobile phase consisted of A (0.1% formic acid) and B (0.1% formic acid in acetonitrile).…”

Section: Hplc Analysis Of Vitexin and Naringeninmentioning

confidence: 99%

Phenolics-Enhancing Piper sarmentosum (Roxburgh) Extracts Pre-Treated with Supercritical Carbon Dioxide and its Correlation with Cytotoxicity and α-Glucosidase Inhibitory Activities

Hamil,

Zakaria,

Chong Yew

et al. 2023

JST

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Piper sarmentosum was found to have various phytochemicals such as polyphenols and flavonoids but also contains asarone isomers, which were reported as carcinogenic in several preclinical studies. Therefore, reducing asarone isomers from this functional food is important while retaining its functional properties. This study compares the total phenolics, total flavonoids, vitexin and naringenin contents, cytotoxicity, and anti-hyperglycaemic activity between various solvent extracts using a two-step extraction with SC-CO2 pre-treatment and a single-step conventional solvent extraction without SC-CO2 pre-treatment. The findings showed that phenolic content was significantly enhanced in ethanol, 50% ethanol, and water extracts pre-treated with SC-CO2, significantly correlated with α-glucosidase inhibitory activity. SC-CO2 pre-treated extracts enhanced the viability of two normal fibroblasts NIH/3T3 and CCD-18Co cell lines. It is concluded that SC-CO2 extraction offers a rapid pre-treatment step to produce safer extracts with better quality and efficacy.

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The metabolites of Piper sarmentosum and their biological properties: a recent update

Adib,

Salmin,

Kasim

et al. 2024

Phytochem Rev

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Simultaneous Determination of Two Isomers of Asarone in <i>Piper sarmentosum</i> Roxburgh (Piperaceae) Extracts using Different Chromatographic Columns

Cited by 3 publications

References 19 publications

Optimisation of asarone removal from Piper sarmentosum Roxburgh leaves using supercritical carbon dioxide extraction: the Box-Behnken design

Optimisation of asarone removal from Piper sarmentosum Roxburgh leaves using supercritical carbon dioxide extraction: the Box-Behnken design

Phenolics-Enhancing Piper sarmentosum (Roxburgh) Extracts Pre-Treated with Supercritical Carbon Dioxide and its Correlation with Cytotoxicity and α-Glucosidase Inhibitory Activities

The metabolites of Piper sarmentosum and their biological properties: a recent update

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