This study was conducted to determine the effects of three different fat sources and their combination on growth performance, carcass traits and intestinal measurements of broiler chickens reared to 42 d of age. Two hundred day-old male broiler chicks (Ross 308) were randomly assigned to one of five treatments with four replicates of 10 chicks based on a completely randomized design. The dietary treatments consisted of 4% added fat from three different sources and their combination as follows: T, diet containing 4% tallow; CO, diet containing 4% canola oil; SFO, diet containing 4% sunflower oil; TCO, diet containing 2% tallow+2% canola oil; TSFO, diet containing 2% tallow+2% sunflower oil. Dietary fat type affected significantly BW and gain as well as feed efficiency in birds fed the TCO diets compared with those fed the other diets. Dietary fat type also modified meat yield, resulting in a higher breast and drumstick yields in the birds fed TCO and TSFO diets, respectively. Most of internal organ relative weights and small intestine measurements were not influenced by dietary treatments, except for the abdominal fat pad weight that was lower in birds fed SFO and for small intestinal length that was influenced by fat source. Results from the current study suggested that the supplementation with a combination of vegetable and animal fat sources in broiler diet supported positively growth performance and carcass parameters.

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The aim of the present study was to evaluate the effects of lemon balm (LB; Melissa officinalis L.) extract as additive on performance, health status and carcass traits of broilers during a 42-days production cycle. One hundred mixed chicks of Ross 308 strain were assigned for five dietary treatments with four replicates per group and five birds per replicate as follows: control diet, 0.5LB, 1.0LB, 1.5LB and 2.0LB with 0.0, 0.5, 1.0, 1.5 or 2.0 mL of LB extract per liter of drinking water, respectively. In overall, at 42nd day, low feed, energy and protein efficiency (P＜0.05) were observed in 1.0LB group than in control diet. However, during the 3rd and 5-6th weeks, feed, energy and protein intakes were improved (P＜0.05), without any efficiency enhancement (P＞0.05) mainly in group on 1.0LB diet. During the 5th week of rearing, daily weight gain was higher (P＜0.05) in groups 0.5LB, 1.0LB and 2.0LB compared to control diet. At the end of feeding period, cecal enterococcus bacteria colony count was higher (P＜ 0.05) and left cecum diameter was lower (P＜0.05) in 1.0LB group. Hematological parameters and viscera and carcass traits remained unaffected (P＞0.05) by dietary treatments. In conclusion, the supplementation with LB as natural feed additive resulted in a potential positive effect on broilers performance mainly during the grower and finisher periods.

The mulberry tree (Morus alba) is a perennial and fast-growing tree distributed worldwide under different climatic conditions. Most of the world’s silk production (>90%) is facilitated by the feeding of silkworm larvae on the leaves of mulberry (Morus alba L.) varieties. Therefore, exploration of the protocol for improving the propagation efficiency and increasing the reproductive capacity of M. alba varieties could be of great significance. This study aimed to determine the effect of four concentrations (0, 100, 200 and 400 mg L−1) each of naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), and zinc sulfate (0, 100 and 200 mg L−1), supplied separately or combined, on the rooting and growth of mulberry cuttings. M. alba cuttings were immersed for 5 s in each solution using the quick-dip method and subsequently, the cuttings were dried and planted in plastic pots and maintained in a greenhouse for 60 days. The number of leaves (NL), longest root size (LRS), longest stem size (LSS), number of rooted cuttings (NRC), number of stems per tree (NSP), rooting percentage (RP), wet root weight (WRW), dry root weight (DRW), wet stem weight (WSW), dry stem weight (DSW), wet leaf weight (WLW) and dry leaf weight (DLW) were evaluated. The results obtained showed an increase in all growth parameters of the mulberry cuttings. Treatments of hormones (IBA and NAA) and Zn sulfate were effective on LSS, LRS and WSW. The highest values of LSS were obtained for the treatments T5, T6, T14, T15, T16 and T18. Moreover, T5, T12 and T10 showed the highest values of LRS. The highest value of WSW was observed for T18, T5, T14, T15 and T16. The highest values of WLW and DLW were observed in T20 and T14. Dry stem weight (DSW) was high in T18 and T14. The application of NAA (at 200 mg L−1), IBA (200 and 400 mg L−1) and Zn sulfate (200 and 400 mg L−1), either alone or in double combination, can be a suitable and reliable method for mulberry propagation.

The effect of Lactofeed probiotic and different sources of fat on performance, carcass characteristics, intestinal morphology and ghrelin gene expression of broiler chickens was studied in an experiment using a total of 240 one-day-old male chickens from commercial strain (Ross 308) in a completely randomized design via 6 treatments with 4 replicates (10 birds per replicate). The experimental diets included: (1) basal diet (control); (2) diet containing 3% animal fat from tallow (fat); (3) diet containing 3% plant oil from soybean (oil); (4) control + probiotic; (5) probiotic + (fat) and (6) probiotic + (oil). The results showed some improvement in performance in the third group (P<0.05). A significant difference in the length, width and depth of crypt was observed between the treatments 3 and 4, and the control group (P<0.05). There was a significant difference in ghrelin gene expression of the treatments 2 and 4 in comparison with the control group (P<0.05). The results generally showed that there were benefits from the separate use of probiotic and soybean oil in the diet of broiler chicken.