Background. The goal of the study is to investigate the association of IL-4-590 and IL-13-1112 genetic polymorphisms with type 2 diabetes mellitus (T2DM) in Egyptian patients. Subjects and Methods. The study included 135 cases with T2DM and 75 healthy unrelated age-matched controls from the same locality of Egypt. DNA was extracted and processed by the ARMS-PCR technique for characterization of genetic variants of IL-4-590 C>T and IL-13-1112 C>T polymorphisms. Results. Egyptian cases with T2DM showed a lower frequency of the IL-4-590 CC homozygous genotype compared to controls (10.4% versus 43.48%) with a higher CT heterozygous genotype (85.2% versus 47.8%). Similarly, cases showed a lower frequency of the IL-13-1112 CC genotype (20.7% versus 56.8%) with a higher frequency of the heterozygous IL-13-1112 CT genotype (76.3% versus 41.3%). Both polymorphisms showed significantly positive associations with T2DM in the dominant, codominant, and overdominant models of inheritance. On the other hand, comparing genotypes of subgroups related to gender, positive family history, and positive consanguinity showed a nonsignificant difference (P > 0.05). Conclusion. Heterozygous genotypes (IL-4-590 CT and IL-13-1112 CT) could be considered as risk factors, while the homozygous wild types (-590 CC and -1112 CC) might be considered protective to T2DM.
Background: Type 2 diabetes mellitus is the most frequent type of diabetes. It is caused by insulin resistance and often combined with symptoms of progressive defect in insulin secretion. Various factors have been implicated in the pathogenesis and complications of type 2 diabetes mellitus (T2DM), of which, immune response and inflammation were suggested to play a role. Objective: To investigate the association of IL-4-590 and IL-13-1112 genetic polymorphisms with (T2DM) in Egyptian patients. Subjects and Methods: The study included 135 cases with type 2 DM (65 males and 70 females), with a median age of 56 years and 101 healthy unrelated controls from Nile Delta region, Egypt. DNA was extracted by purification Capture column kit supplied by Fermentas, K0721, USA. Polymorphisms of IL-4-590(C>T) and IL-13-1112(C>T) genes were characterized using ARMS-PCR technique. Results: The frequency of heterozygous (CT) genotype of IL-4-590 was significantly increased in type 2 diabetic patients compared to the control, CT genotype of IL-13-1112 was highly significantly increased in diabetic patients compared to control (85.2 vs.66.3, OR=2.9, p=0.001; 76.3 vs.51.5, OR=3' p= 0, respectively). The frequency of the homozygous (CC) genotypes of both IL-4 and IL-13 were significantly lower in type 2 diabetic patients than in the control group
BackgroundInterleukin-10 (IL-10) is a multifunctional regulatory cytokine that might be associated with increased risk of type 2 diabetes mellitus (T2DM). IL-10 gene polymorphisms have been reported to be associated with T2DM in several ethnic populations with controversial results.ObjectivesThis work is an updated meta-analysis aiming at the evaluation of the association between IL-10 gene polymorphisms: rs1800872 (− 592 C > A), rs1800896 (− 1082 A > G) and rs1800871 (− 819 C > T) with the risk of T2DM.MethodsAll available full text studies published up to July 2015 were included in this meta-analysis. Mainly Pubmed and Science Direct databases were searched for all eligible studies pertinent to testing the association between IL-10 gene polymorphisms with the susceptibility to T2DM. Further analyses of the pooled and stratified data in terms of individual polymorphic types and subject ethnicity were done and assessed using varied genetic models.ResultsFifteen case-control studies with a total of 26 comparisons (10 for IL-10 − 592 C > A rs1800872, 11 for IL-10 − 1082 A > G rs1800896 and 5 for IL-10 − 819 C > T rs1800871 polymorphisms) met our inclusion criteria. IL-10 − 1082 A > G polymorphism was the only one to show an association with T2DM in all pooled sample particularly among Asian and European (high frequency of the G allele) ethnic groups. On the other hand, IL-10 − 592 C > A and − 819 C > T were significantly associated with T2DM only among African subjects.ConclusionsThis meta-analysis demonstrated that IL-10 − 1082 A > G polymorphism was associated with increased risk of development of T2DM in total subjects no matter was their ethnic background, while both IL-10 − 592 C > A and − 819 C > T polymorphisms were associated with that risk only among African subjects.
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