ABSTRACT:The prevalence of brucellosis was determined in the ruminants (buffaloes, cattle, sheep and goats) of five different districts viz. Bagerhat, Bogra, Gaibangha, Mymensingh and Sirajgonj of Bangladesh. A total of 550 sera samples of 105 buffaloes, 188 cattle, 127 goats and 130 sheep were screened by RBT and were further confirmed with I-ELISA. A structured questionnaire was used to collect epidemiological information on the animals. The overall serological prevalence derived from the samples was 2.87% in buffaloes, 2.66% in cattle, 3.15% in goats, and 2.31% in sheep. The prevalence was relatively higher in females than that in males in cattle, goats and sheep but, an insignificantly higher prevalence was observed in males than that in females in the case of buffalo. A significant association was found between abortion or age and occurrence of brucellosis (P < 0.01). The results of the study provide (a) a comparison of the prevalence of brucellosis in different livestock species in Bangladesh, (b) constitute baseline data for further study of Brucella infections, and (c) are a starting point for the control of brucellosis.
The aim of the present study was to determine the responses of Black Bengal Goat following synchronization with alfaprostol, superovulation with PMSG and embryo collection with surgical procedure. This research work was carried out at the Department of Surgery and Obstetrics, Bangladesh Agricultural University (BAU) during the period from January to December 2001. Three Flushing schedules have been carried out in one-year time consisting of 5 donor and 4 recipients in Flush I, 2 donors and 4 recipients in Flush II, and 4 donors and 3 recipients in Flush III. The donors as well as the recipients were synchronized with Gabbrostim ® (Alfaprostol, VETEM, Italy) at the dose rate of 2-mg equivalent to 1ml/goat. Among 11 donors only 6 donors responded following first injection, but all donor responded with second and third injection of Gabbrostim ® . The mean time of onset of oestrus and duration of oestrus in donor were 29.9 ± 9.20h, 50.0 ± 5.94h, 47.8 ± 4.05h and 20.9 ±5.10h, 27.9 ± 1.19h, 29.4 ± 1.36h respectively. Among 11 recipient does, only 6 responded following first injection of Gabbrostim, while that of second injection was 9. The mean time of onset of oestrus and duration of oestrus were 28.6 ± 9.69h, 40.1 ± 8.95h and 17.1 ± 5.05h, 22.5 ± 3.45h respectively. All 9 recipients responded in third injection and the mean time of onset of oestrus and duration of oestrus were 39.9 ± 5.06h and 27.4 ± 1.32h respectively. In all flushing schedules, superovulation was performed using PMSG (Folligon ® , Intervet International B.V, Holland) at the dose rate of 900 IU, 800 IU and 700 IU respectively. Both natural service and AI was performed to fertilise the ovum after onset of oestrus. The superovulatory response was determined by counting the number of corpora lutea (CL) during collection of embryos. The percent of superovulatory donor goats were 100, 50 and 25% respectively. The mean number of ovulation was 13.6 ±3.6, 10.5 ± 8.5 and 3.5 ± 3.2 respectively. The embryos were collected surgically by using 50 ml Phosphate buffer saline (PBS). The embryos were counted and graded under stereomicroscope. The embryo collection rates varied from 0-100%. The recovered embryos were of 100% fertile. The total number of embryos recovered was 4, 8 and 8 with the percent of recovery 6, 38 and 61.5% respectively. In Flush I among 4 recovered embryos, 3 were Grade 1 morula and the rest embryo was Grade 2 late morula. In Flush II among 8 recovered embryos, 7 were Grade 2 early morula and rest embryo was Grade 2 late morula. In Flush III among 8 recovered embryos, 5 were Grade 2 morula and rests 3 were Grade 2 late morula.
Aim:This study was performed to identify the non-cerebral Taenia multiceps cyst through molecular phylogeny of the 12S rRNA gene.Materials and Methods:Eight cyst samples were collected from 385 examined slaughtered goats during October 2015-September 2016 from three slaughterhouses in Chittagong City Corporation. Cysts were removed from the thigh muscle, and scolices were collected for light microscopic examination and molecular identification. The DNA was extracted and analyzed by polymerase chain reaction using 12S rRNA gene primers. Cyst samples were also preserved in 10% buffered formalin for histopathological study.Results:T. multiceps non-cerebral cyst is 2.1% prevalent in goat in this area. Under light microscopic examination, scolex was found with four suckers and a rostellum with the double crown of 32 hooks and hooklets. Molecularly, all the samples were amplified with 12S rRNA gene fragments yielded 270 base pair amplicon. Zenker’s necrosis with focal to diffuse infiltration of lymphocytes and eosinophil was also found around the cyst wall in histopathological examination.Conclusion:Although the non-cerebral form of the cysts produced by T. multiceps is genetically identical with the cerebral cyst, previously published data indicated that cerebral T. multiceps cyst is predominant in other parts of the world as well as in Bangladesh. This study showed that non-cerebral cyst is also prevalent in this country which is very important for public health concern. This study depicts an idea of non-cerebral form of zoonotic T. multiceps cyst which will be helpful in taenia cyst control and prevention.
The aim of this study was to isolate and identify uterine microorganisms in postpartum cows and to observe the occurrence of uterine infection in crossbred and local cows. One hundred and nine samples, from both crossbred and local dairy cows were collected from Chittagong area. They were inseminated artificially (n=89) and naturally (n=20). Samples were collected at least 3 months after calving. Among the selected cows, 33 had different reproductive diseases and the rest were from apparently healthy cows. The crossbred dairy cows were more susceptible to reproductive diseases (19.27%) than the local ones. Uterine infection was higher (52%) in cows inseminated artificially than that of natural ones (21.21%). Nineteen different types of bacteria were isolated from both diseased and healthy animals, except Clostridium spp and Nocardia spp which were not found in diseased animal. Likewise, Bacillus spp and Salmonella spp were absent in healthy animals. Most prevalent bacteria in diseased animal were Staphylococcus spp and Streptococcus spp found in 33% and 27.3% of the cases respectively. In non-diseased cases, the highest number of samples was positive for Bacteroied spp (13.2%) followed by Streptococcus spp (10.4%). On average, the highest number of bacterial population was isolated from diseased samples and most of those bacteria are pathogenic.
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