Background and Aim: Pleomorphic adenoma gene 1 (PLAG1) encodes a multifunctional transcription factor that controls many genes and pathways and is associated with cattle body weight and measurements. This study aimed to evaluate the association between PLAG1 polymorphisms with body weight and measurements in Bali cattle. Materials and Methods: A total of 87 Bali cattle, consisting of 48 bulls and 39 heifers at the Breeding Center for Bali Cattle, were used as the population in this study. Cattle were 2 years old and kept semi-intensively in the pasture. Phenotype data consisting of body weight, withers height, body length, chest girth, waist height, and chest depth were measured. Birth weight data were obtained from birth records, and weight gain, adjusted weaning weight, and yearling weight were calculated using formulas. Blood samples were taken from the jugular vein as much as 5 mL, and genomic DNA was isolated using the salting-out method. Polymerase chain reaction (PCR) was performed to amplify three target polymorphisms, namely, g.48308 C>T, g.32212 (19 bp indel), and g.45233 T>C. The presence of a 19 bp indel was determined by direct observation of the PCR product on a 2% agarose gel. Two other polymorphisms were detected by PCR-restriction fragment length polymorphism using the restriction endonuclease enzymes SacII and BclI. PLAG1 genotype and phenotype associations were analyzed using a general linear model. Results: The results showed that two of the target polymorphisms in PLAG1 did not vary. The DD genotype indicated by 123 bp of PCR product was the only genotype identified for g.32212 19 bp indel, and TT genotype was the only genotype found for g.45233 T>C single-nucleotide polymorphism (SNP). Conversely, g.48308 C>T SNP was found to be polymorphic. In addition, the g.48308 C>T polymorphism of PLAG1 was significantly associated with body length of Bali cattle. Cattle with the CC genotype had a greater body length than the other two genotypes. Conclusion: The g.48308 C>T SNP in PLAG1 was associated with Bali cattle body length characteristics. This finding could be used as a basis for selecting Bali cattle based on body length characteristics.

<p><strong>Objective: </strong>The objective of this study was to analyze <em>CSN2</em> variants in Indonesian Friesian Holstein (FH) cow and their association with milk protein allergy and production traits.</p><p><strong>Methods: </strong>Genomic DNA was extracted from bloods of twelve Indonesian FH cow. Exon 7 of the <em>CSN2</em> was amplified using novel primer pair to produce 683 bp amplicon. The primers were 5’-ACCCCAATTTCTTAACCAAACCA-3’ as a forward primer and 5’-CATCAGAAGTTAAACAGCACAGT-3’ as a reverse primer. The PCR products were analyzed to determine the nucleotide sequence of <em>CSN2</em> using Bioedit version 7.2.5. Moreover, Hardy-Weinberg equilibrium (HWE) was calculated and one-way analysis of variance (ANOVA) was conducted to associate between <em>CSN2</em> variants and milk production traits.</p><p><strong>Results: </strong>Two polymorphisms, c.350A&gt;C and c.516G&gt;C, were identified in the <em>CSN2 </em>exon 7. Base substitution from adenine (A) to cytosine (C) of c.350A&gt;C changed amino acid codon from histidine (CAU) to proline (CCU), and base substitution guanine (G) to cytosine (C) of c.516G&gt;C changed amino acid codon from arginine (AGG) to serine (AGC). The CC genotype frequency for c.350A&gt;C SNP was 33% and they were able to produce A2 <em>CSN2</em> variant which is favorable for preventing lactose intolerance. In addition, there were no association between c.350 A&gt;C and c.516 G&gt;C SNP of the <em>CSN2</em> with milk production traits.</p><p><strong>Conclusions: </strong>In conclusion, A1 and A2 variants of <em>CSN2</em> were identified in Indonesian FH population and they did not associate with milk production in Indonesian FH.</p><p> </p>