In this study, micro propagation of two Iranian fenugreek populations and their morphological and biochemical responses to salinity and drought stresses in in vitro culture condition were conducted using factorial experiment in a completely randomized design in three replications. Different explant type (terminal bud, cotyledon and epicotyledon explant) were cultured in MS medium contain different concentration of plant growth regulators such as kin (0, 0.5 and 1 mg / l) and 2,4-D (0.5, 1 and 2 mg / l). Murashige and Skoog (MS) medium supplemented with 1 mg/l kinetin and 2 mg/l 2,4-D showed the highest callus proliferation rate per explants in both populations. The highest amount of callus volume was obtained from the explants of the terminal bud. Proliferated calli from terminal bud explant were green and yellowish, from cotyledon were yellowish to white with soft texture, and the cotyledons were greenish and compact. The results of salinity stresses with different concentrations of sodium chloride (0, 70 and 120 mM) and drought stress with polyethylene glycol (0, 5 and 10%) showed that both stresses decreased callus growth and increased total protein, proline, catalase, peroxidase and trigonelin content in both populations. Trigonelin measurement showed that ‘Borazjan’ papulation had higher trigonelin content, in vitro, than ‘Ardestan’ papulation.
and placement on yield and zinc utilization by blackbean ( d6termin6 les rapports entre les teneurs en zinc des plantes, I'utilisation du zinc par les plantes et la concentration du zinc dans la zone de rdaction du sol. Une 6quation a6t6, €labor4rn, afin d'6tablir les rapports entre les utilisations du zinc et la concentration du zinc dans la bande de terre fertilis6e et nous avons ainsi pu ddterminer comment une augmentation de la superficie de la zone fertilisee influe sur la teneur en zinc des plantes. Nos r6sultats donnent i conclure que le zinc inorganique doit €tre m6lang6 le plus uniform6ment possible pour donner une efficacit€ maximale.
St. John's wort is a traditional medicinal plant that has been used from ancient time for the treatment of neurological and psychiatric diseases. A successful in vitro propagation system for the St. John's wort was optimized. Different explant type (single node and stem cutting with two nodes) were used in Murashige and Skoog (MS) medium in different concentrations (MS full, MS and MS) supplemented with BA (. ,. ,. mgl-) and sucrose (and gl-). The optimum proliferation rate of St. John's wort was obtained in MS medium containing glof sucrose and. mgl-BA with single node explant. Also, evaluation of results revealed that the number of proliferated shoots will increased in MS media supplemented with BA along with reduced concentrations of sucrose. The in vitro generated shoots were cultured on half-strength MS medium containing ,. ,. ,. ,. and mglbutyric acid (IBA). The results showed that all proliferated shoot were rooted in plant growth regulators free medium. But, using and increasing the concentration IBA in rooting media can increases the number of roots and average of root length per each explant.
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