Chemiluminescence immunoassay (CLIA) has been widely applied in different fields including environmental monitoring, liquid chromatography, clinical diagnosis; food safety, pharmaceutical analysis, immuno-and gene probe assays, as a promising approach for selective, sensitive, rapid and simple analysis. It is often necessary to detect a large number of complicated or low-abundance samples. However, traditional methods need great consumption of time, reagents and labor, which limit their clinical applications. As a result, rapid, high-throughput, sensitive and low-cost detection methods have become the development trend of CLIA. A new chemiluminescence immunoassay analyzer with advanced acridinium ester (AE) technology will be installed at the radioimmunoassy laboratory of National Institute of Nuclear Medicine and Allied Sciences (NINMAS) shortly. In conjunction with the existing Radioimmunoassay (RIA), Immunoradiometric assay (IRMA) and DissociationEnhanced Lanthanide Fluorescent Immunoassay (DELFIA), introduction of chemiluminescence immunoassay (CLIA) technology will expand the activities of in vitro immunoassay. It will also improve the research and development activities of in vitro division of NINMAS. In this review article, we have summarized the basic principle of chemiluminescence immunoassay and its applications. A comparison of RIA, CLIA and enzyme-linked immunosorbent assay (ELISA) was also briefly described in the article.
Background: Newborn screening programs signify early and presymptomatic detection of treatable disorders which authorize commencement of prompt medication to alleviate notable fatality. Newborn screening (NBS) for congenital hypothyroidism (CH) has been a successful public health initiative in preventive medicine over the last few decades. Previous pilot (1999-2006) and phase 1 (2006-2011) NBS program in Bangladesh reported an increased incidence compared to global data which urged to initiate a second phase NBS program for the detection of CH. Materials & Methods: Neonates’ blood was collected from the umbilical cord or by heel prick and drawn on the Guthrie filter paper card. Thyroid-stimulating hormone (TSH) was measured from this filter paper by Immunoradiometric assay (IRMA) or Dissociation Enhanced Lanthanide Fluorescence Immunoassay (DELFIA) method. Results: 123 out of 2,61,550 neonates have already been diagnosed with congenital hypothyroidism from September 2018 to August 2020, which represents an incidence of 1:2126. Conclusion: Ongoing phase-2 project data is already showing some optimistic outcomes. Hence, a time-worthy sustainable policy should be implemented to mark this program as an effective one. Bangladesh J. Nuclear Med. 25(1): 15-21, 2022
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