The effects of chronic exposure to waterborne Cd and elevated dietary Ca, alone and in combination, were examined Ž . w in juvenile rainbow trout, Oncorhynchus mykiss. Fish were chronically exposed to 0.05 control or 2.56 grl Cd as Ž . x Ž . Ž Cd NO и 4H O and were fed 2% body massrday of control 29.6 mg Carg or Ca-supplemented trout food 52.8 mg uptake from water into the gill and induced hypocalcemia in the plasma on day 40. Waterborne Cd also induced an elevated Ca concentration on day 20 in the gill tissue of trout fed the Ca-supplemented diet and a decreased Ca concentration on day 35 in the gills of trout fed the control diet. Dietary Ca protected against Cd accumulation in gill, liver, and kidney, but did not protect against the inhibition of Ca uptake into the gill or plasma hypocalcemia. When fed Ca-supplemented diet and exposed to waterborne Cd, fish showed 35% mortality, compared to 0᎐2% in control fish and in the Cd-exposed fish with normal Ca in the diet. Growth, on the other hand, was not affected by any treatment. ᮊ