Dietary Ca inhibits waterborne Cd uptake in Cd-exposed rainbow trout, Oncorhynchus mykiss

Zohouri, Mohammad Ali; Pyle, Greg G.; Wood, Chris M.

doi:10.1016/s1532-0456(01)00260-5

Cited by 24 publications

(17 citation statements)

References 35 publications

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“…Similar results were found in tilapia (Oreochromis niloticus) fed diets containing Cd from 5 to 100 µg.g -1 dry food, in which Cd content in feces were ≤ 279 µg.g -1 (Nogami et al, 2000). Although high concentrations of Ca 2+ in either the water or the diet clearly have ameliorative effects on waterborne Cd toxicity in fish (e.g., Hollis et al, 2000;Zohouri et al, 2001;Baldisserotto et al, 2005), it is unclear whether the low water Ca 2+ concentration in the holding tanks (~10 µmol.L -1 ) contributed to amplify the dietary Cd accumulation in tambaqui. The sensitivity of tambaqui to waterborne Cd has been reported in a previous study (Matsuo et al, 2005), and the effect of soft water on dietary Cd accumulation still needs further investigation.…”

Section: Discussionsupporting

confidence: 69%

Dietary tissue cadmium accumulation in an amazonian teleost (Tambaqui, Colossoma macropomum Cuvier, 1818)

Matsuo

Val

2007

Braz. J. Biol.

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Understanding the effects of metal contamination in the Amazon basin is important because of the potential impact on this region of high biodiversity. In addition, the significance of fish as the primary source of protein for the local human population (living either alongside the Amazon River or in the city of Manaus) highlights the need for information on the metal transfer through the food chain. Bioaccumulation of metals in fish can occur at significant rates through the dietary route, without necessarily resulting in death of the organism. The goal of this work was to expose an economic relevant species from the Amazon basin (tambaqui, Colossoma macropomum) to dietary cadmium (Cd) at concentrations of 0, 50, 100, 200, and 400 µg.g -1 dry food. Fish were sampled on days 15, 30, and 45 of the feeding trials. Tissues were collected for analysis of Cd concentration using graphite furnace atomic absorption spectrophotometry. Cd accumulation in the tissues occurred in the following order: kidney > liver > gills > muscle. Relative to other freshwater fish (e.g., rainbow trout, tilapia), tambaqui accumulated remarkably high levels of Cd in their tissues. Although Cd is known to affect Ca 2+ homeostasis, no mortality or growth impairment occurred during feeding trials.Keywords: dietary cadmium, tissue accumulation, Colossoma macropomum, Amazon, soft water. Acúmulo dietário de cádmio em tecidos de um teleósteo Amazônico (Tambaqui, Colossoma macropomum)Resumo O entendimento dos efeitos da contaminação por metais na Bacia Amazônica é importante devido ao potencial impacto sobre esta região de elevada biodiversidade. Além disso, a relevância dos peixes como fonte primária de proteína para a população humana local (tanto nas comunidades ribeirinhas ao longo do rio Amazonas, quanto na cidade de Manaus), ressalta a necessidade de informação sobre a transferência de metais através da cadeia alimentar. Bioacumulação de metais em peixes pode ocorrer em taxas significativas através da dieta, sem necessariamente resultar na morte do indivíduo. O objetivo deste estudo foi expor cronicamente uma espécie de importância comercial nativa da Amazônia (tambaqui, Colossoma macropomum) a dietas enriquecidas com cádmio (Cd) em concentrações de 0, 50, 100, 200, and 400 µg.g -1 alimento seco. Os peixes foram amostrados nos dias 15, 30 e 45 do tratamento experimental. Os tecidos foram coletados para análise quanto a concentração de Cd por meio de espectrofotometria de absorção atômica acoplado a forno de grafite. O acúmulo de Cd nos tecidos ocorreu na seguinte ordem: rim > fígado > brânquias > músculo. Comparando-se com outras espécies de peixes de água doce (por exemplo, truta arco-íris, tilápia), o tambaqui acumulou níveis de Cd extremamente mais elevados em seus tecidos. Apesar do Cd ser conhecido por afetar a homeostase do Ca 2+ , não houve mortalidade ou retardo no crescimento durante os testes dietários.Palavras-chave: Cádmio, bioacumulação tecidual, Colossoma macropomum, Amazônia, água doce.

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Section: Discussionsupporting

confidence: 69%

Dietary tissue cadmium accumulation in an amazonian teleost (Tambaqui, Colossoma macropomum Cuvier, 1818)

Matsuo

Val

2007

Braz. J. Biol.

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“…In agreement to our finding, previous finding from Muramoto (1981) has also showed that Cd ?2 could influence bone metabolism and may disturb its structure and causes hypocalcaemia. Zohouri et al (2001) showed that waterborne Cd ?2 induce hypocalcaemia in rainbow trout fish. Hypocalcaemia was also reported as a most sensitive effect of Cd ?2 toxicity in common carp during Cd ?2 exposure (Hans et al 2006).…”

Section: Discussionmentioning

confidence: 99%

“…Muramoto (1981) reported the appearance of bone problem and specifically Ca deficiency in common carp following Cd ?2 exposure. Investigations by many other researchers have also confirmed that Cd ?2 induced hypocalcaemia in fish (Haux et al 1988;Zohouri et al 2001;Hans et al 2006) and has altered the serum ALP level (Sastry and Subhadra 1985;Gill et al 1991;Al Attar 2005;Velmurugan et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of zinc on related parameters to bone metabolism in common carp fish (Cyprinus carpio L.) intoxified with cadmium

Malekpouri

Moshtaghie

Kazemian

et al. 2010

Fish Physiol Biochem

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The short term effects of waterborne cadmium (Cd(+2)) on the levels of serum parameters related to bone metabolism including calcium (Ca), inorganic phosphorus (P(i)) and alkaline phosphatase (ALP) in common carp fish (Cyprinus carpio L.) were studied. Fish were treated with varying concentrations of Cd(+2) (0.22, 1.1 and 2.2 mg l(-1)) daily for 14 days. The results obtained show that serum P(i) and ALP concentrations were elevated by increasing Cd(+2) concentration in water containing fish whereas serum Ca level was decreased. At the same time, the protective role of waterborne zinc (Zn(+2), 1 mg l(-1)) on the same parameters was also investigated. Results showing that daily treatment of fish with Zn(+2), increased the concentrations of Ca and ALP in serum by 2.07 and 1.86 fold and decreased serum P(i) level by 57.7% in comparison with Cd(+2) treatment. The combination of Cd(+2) and Zn(+2) on the same parameters was studied next. There was a significant (P < 0.05) elevation in serum Ca and ALP levels in comparison with Cd(+2) treatment. Decreasing in serum P(i) level was not significant in comparison with Cd(+2) treatments. The protective effect of Zn(+2) on Cd(+2) disturbances in serum parameters related to bone metabolism in this manuscript has been also discussed.

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“…There is an interesting parallel here to cadmium metabolism, which is thought to be taken up across the gill via apical Ca 2+ channels in the ionocyte (Verbost et al, 1989). Recently, Zohouri et al (2001) reported that elevated dietary Ca 2+ reduced but did not eliminate branchial cadmium uptake in rainbow trout.…”

Section: Discussionmentioning

confidence: 99%

Dietary sodium inhibits aqueous copper uptake in rainbow trout(Oncorhynchus mykiss)

Pyle

Kamunde

McDonald

et al. 2003

Journal of Experimental Biology

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SUMMARYOurs is the first study to demonstrate an influence of dietary sodium on waterborne copper uptake in fish. We examined possible interactions between dietary sodium and the response of freshwater rainbow trout (Oncorhynchus mykiss) to waterborne copper in light of recent evidence of interactions between sodium and copper metabolism in the gills. Trout were maintained for 6 days on one of four diets of increasing sodium concentration (0.25 mmol g-1, 0.51 mmol g-1, 0.76 mmol g-1 and 1.27 mmol g-1, which corresponds to 0.6%, 1.2%, 1.8% and 3% sodium by mass, respectively). At the end of 7 days, fish were exposed for 6 h to waterborne copper spiked with 64Cu to determine if the dietary sodium affected responses to a subsequent short-term waterborne copper exposure. The radiotracer allowed us to distinguish between Cu occurring in fish tissues before the experiment and `newly accumulated' Cu arising from the experimental exposure. Dietary sodium concentrations of 1.8% or 3% reduced newly accumulated copper concentrations in gill (from 93.9 ng g-1in control to 38.9 ng g-1 and 20.0 ng g-1 in fish fed 1.8% or 3% Na+-supplemented diets, respectively), liver (from 64.3 ng g-1 to 23.1 ng g-1 and 7.5 ng g-1,respectively), kidney (from 29.3 ng g-1 to 11.7 ng g-1and 7.8 ng g-1, respectively), plasma (from 64.7 ng g-1to 21.5 ng g-1 and 10.7 ng g-1, respectively) and gut(from 6.8 ng g-1 to 3.4 ng g-1 and 2.2 ng g-1, respectively) by 50.0-88.2%. The 3%Na+-supplemented diets also increased plasma and gut sodium concentrations by 38.1% (from 137.1 μmol g-1 to 189.3 μmol g-1) and 104.3% (from 56.5 μmol g-1 to 115.4 μmol g-1), respectively, relative to fish maintained on untreated diets. Whole body uptake rates of both sodium and copper were significantly reduced,and highly correlated (r=0.97) with one another, in fish fed high-sodium diets relative to controls. Moreover, sodium efflux was 12% and 38% higher in fish fed 1.8% and 3% sodium-enriched diets, respectively. Fish fed high-sodium diets also drank more water, but the contribution of drinking to waterborne copper uptake was negligible. From these results, we speculate that, at least in part, aqueous sodium and copper share a common branchial uptake route, probably through an apical sodium channel. According to this hypothesis, as the channel is downregulated with increasing internal sodium concentrations, both sodium and copper uptake from the water are inhibited.

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Dietary Ca inhibits waterborne Cd uptake in Cd-exposed rainbow trout, Oncorhynchus mykiss

Cited by 24 publications

References 35 publications

Dietary tissue cadmium accumulation in an amazonian teleost (Tambaqui, Colossoma macropomum Cuvier, 1818)

Dietary tissue cadmium accumulation in an amazonian teleost (Tambaqui, Colossoma macropomum Cuvier, 1818)

Protective effect of zinc on related parameters to bone metabolism in common carp fish (Cyprinus carpio L.) intoxified with cadmium

Dietary sodium inhibits aqueous copper uptake in rainbow trout(Oncorhynchus mykiss)

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