Spectrophotometric Determination of Tranexamic Acid by Azo-Dye … 22 was obeyed within (0.5-10) ppm with a molar absorptivity 3.3×10 3 l.mole-1 .cm-1. All variables were studied to optimise the reaction conditions. No interference was observed in the presence of common pharmaceutical excipients. The validity of method was tested by analyzing tranexamic acid in its pharmaceutical preparations and good recoveries were obtained.

A spectrofluorimetric method has been developed for the determination of tetracycline hydrochloride and terbutaline sulphate in different dosage forms. The method is based on the quantitative quenching effect of tetracycline hydrochloride and terbutaline sulphate on the native fluorescence of Eosin Y at the pH 6.4 and 3.5 respectively. The quenching of the fluorescence of Eosin Y was measured at 545 nm after excitation at 350 nm. The fluorescence-concentration plots are rectilinear over the range 0.5-18 and 0.05-5.0 μg mL -1 with LOD of 0.531 and 0.241 μg mL -1 and LOQ of 1.77 and 0.806 μg mL -1 for above drugs respectively. The proposed method has been successfully applied to the analysis of commercial tablets and capsules containing the drug. Statistical comparison of the results with those of the reference method revealed good agreement and proved that there were no significant differences in the accuracy and precision between the two methods.* Corresponding Authors E-Mail: dr_theiaa@yahoo.co.uk [a]

A sensitive spectrofluorimetric method has been developed for the analysis of some medicines containing primary, secondary, and tertiary amino groups, namely Diclofenac (DIC), Domperidone (DOM), Famotidine (FAM), and Propranolol (PRO), in their pure and medicinal forms. The method is based on the quenching of the fluorescence intensity of rhodamine 6G (R6G) through the formation of ion-pair complexes between the above medicines and the R-6G reagent, which is measured at 552 nm after excitation at 402 nm. The calibration graphs were rectilinear in the concentration ranges of 0.10- 9.00, 0.05-15.00, 0.10-14.0 and 0.05-5.00 µg mL-1 for above medicines respectively. The recovery (%) values were ranged between 99.45%- 100.97%. The detection limits ranged in the concentration of 0.243-0.754 µg/mL, and the limits of quantitation were 0.806- 2.420 µgmL-1 for all drugs. The method was successfully applied for the determination of these drugs in their pharmaceutical preparations.