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Binding reaction kinetics of analyte-ligand at the level of a sensitive membrane into a microchannel of a biosensor has been limited by the formation of the boundary diffusion layer. Therefore, the response time increases and affects the overall performance of a biosensor. In the present work, we develop an approach to engineer fluid streams into a complex configuration in order to improve the binding efficiency. We investigate numerically the flow deformations around a parallelepiped with square cross-section inside the microfluidic channel and exploit these deformations to simulate the analyte transport to the sensitive membrane and enhance both association and dissociation processes. The effect of several parameters on the binding reaction is provided such as: the obstacle location from the inlet of the microchannel, the average flow velocity, and the inlet analyte concentration. The optimal position of the obstacle is determined. An appropriate choice of the inlet flow velocity and inlet analyte concentration may reduce significantly the response time.

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In this work, we simulate the binding reaction of C-reactive protein in a microchannel of a biosensor. A problem that arises in this device concerns the transport of the analyte toward the reaction surface of the biosensor, which is of a very small dimension. The limitation of mass transport causes the formation of a diffusion boundary layer and restrains the whole kinetic reaction. To enhance the performance of the biosensor by improving the transport, an applied AC electric field and flow confinement are used to stir the flow field. The numerical simulation of these mechanisms on the binding reaction is performed using the finite element method. Swirling patterns are generated in the fluid. They enhance the transport of the analyte and confine it near the reaction surface. The location of the electrode pair on the walls of the microchannel for the design of the biosensor has been studied to find out the effects of varying geometric configurations on the binding efficiency. The best performances of the biosensor are obtained when the electrodes are placed on the same wall of the microchannel as the reaction surface. For the best case, under the effect of the applied electric field alone, the enhancement factors raise up to 2.46 and 2.10 for the association and dissociation phases, respectively. By contrast, under the effect of the electric field with flow confinement, the enhancement factors for the association and the dissociation phases jump to 3.43 and 2.97, respectively, for 30:1 flow confinement (ratio of confining to sample flow).

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