Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics

Selmi, Marwa; Echouchene, Fraj; Gazzah, Mohamed Hichem; Belmabrouk, Hafedh

doi:10.1109/jsen.2015.2475610

Cited by 34 publications

(39 citation statements)

References 22 publications

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“…As expected, the rise in the bulk analyte concentration results in a growth of the biomolecule target quantities trapped on the sensitive surface. The numerical results are in a good agreement with the results reported by Yang et al [13] and Selmi et al [14], where there is a complex quantity formation on the sensitive surface as a function of analyte concentration.…”

Section: Binding Kinetics Of Protein Crpsupporting

confidence: 89%

“…Currently, many scientific researchers are developing various strategies to enhance the mass transport in microfluidic systems and improve the achievement of the target antigen to the sensitive surface. For example, Selmi et al [14] developed a two-dimensional microfluidic confinement device for CRP detection, where the confinement flow velocity grew to enhance the binding rate. Also, Hofmann et al [8] suggested a three dimensional microfluidic for immunoassays on a planar waveguide application, where a sample flow was joined with the perpendicular flow of a sample medium.…”

Section: Introductionmentioning

confidence: 99%

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2D simulation of a microfluidic biosensor for CRP detection into a rotating micro-channel

et al. 2019

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Previously, pumping systems using centrifugal force has been regarded as an attractive control method for fluid flow interior microchannel. In this work, we propose a novel design of a biosensor for complex reactive protein detection in two-dimensional rotating microchannel using the finite element method. We have first developed the physical modeling of the centrifugal force driven, and detailed velocity profile and expended the pressure distribution. Several crucial factors that influence the equilibrium binding time are discussed, including the bulk analyte concentration and the biosensor length. The obtained analytical results reveal that the binding reaction is largely enhanced with the increase of the angular velocity. Hence, an appropriate choice of the angular velocity may enhance the microfluidic biosensor performance and reduce considerably the time response. This work can open new opportunity for further investigation in microflow injection experimental studies.

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Section: Binding Kinetics Of Protein Crpsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

2D simulation of a microfluidic biosensor for CRP detection into a rotating micro-channel

et al. 2019

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“…16 Convective-driven immunosorbent assays function through analyte complexation at antibody-functionalized addresses, 46 typically under nonequilibrium conditions. 47 Depending on binding kinetics, this process may not necessarily be transport limited and results in constantly changing address areas and analyte affinity, and depleted protein concentrations ( C o ) at downstream addresses.…”

Section: Discussionmentioning

confidence: 99%

Calibrant-Free Analyte Quantitation via a Variable Velocity Flow Cell

et al. 2016

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In this paper, we describe a novel method for analyte quantitation that does not rely on calibrants, internal standards, or calibration curves but, rather, leverages the relationship between disparate and predictable surface-directed analyte flux to an array of sensing addresses and a measured resultant signal. To reduce this concept to practice, we fabricated two flow cells such that the mean linear fluid velocity, U, was varied systematically over an array of electrodes positioned along the flow axis. This resulted in a predictable variation of the address-directed flux of a redox analyte, ferrocenedimethanol (FDM). The resultant limiting currents measured at a series of these electrodes, and accurately described by a convective-diffusive transport model, provided a means to calculate an “unknown” concentration without the use of calibrants, internal standards, or a calibration curve. Furthermore, the experiment and concentration calculation only takes minutes to perform. Deviation in calculated FDM concentrations from true values was minimized to less than 0.5% when empirically derived values of U were employed.

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“…There was no significant difference between the curves obtained using these grids (6818, 13643, 27719, 53534 elements) and we may conclude that the numerical convergence has been reached with all the grids. In the results section, all simulations were done with a total elements number 13645 [30,31].…”

Section: A Mesh Sensitivitymentioning

confidence: 99%

Finite-Element Simulations of the pH-ElecFET Microsensors

et al. 2016

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This paper presents a COMSOL Multiphysics 2D axisymmetric model of an pH-ElecFET (pH-sensitive electrochemical field effect transistor) microsensor. This device combines an integrated microelectrode with a pH-sensitive chemical field effect transistor (pH-ChemFET). Thus, by triggering electrolysis phenomena owing to the integrated microelectrode, associated local pH variations in microvolumes are monitored thanks to the pH-ChemFET microdevice. Taking into account (electro) chemical reactions and diffusion phenomena in liquid phase, the proposed model points out the role of the ElecFET geometrical design (microelectrode width w, gate sensitive radius re and distance between the pH-ChemFET gate and the microelectrode d), as well as polarization parameters, (polarization voltage Vp and time tp), on the microsensor response. It is first applied to water electrolysis in order to validate pH impulsional variations in microvolume. Then, oxidation of hydrogen peroxide in phosphate buffer (PBS, pH0=7.2) solutions is studied, evidencing the H2O2 potentiometric detection in the [10-100mM] concentration range. This developed model paves new ways for sensor applications, opening several new opportunities for pH-ElecFET devices for H2O2-related enzymatic detection of biomolecules.

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Flow Confinement Enhancement of Heterogeneous Immunoassays in Microfluidics

Cited by 34 publications

References 22 publications

2D simulation of a microfluidic biosensor for CRP detection into a rotating micro-channel

2D simulation of a microfluidic biosensor for CRP detection into a rotating micro-channel

Calibrant-Free Analyte Quantitation via a Variable Velocity Flow Cell

Finite-Element Simulations of the pH-ElecFET Microsensors

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