The diversity of thermophilic bacteria was investigated in four hot springs,
three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240)
thermophilic bacteria were recovered, identified and characterized. All isolates
were Gram positive, rod-shaped, spore forming and halotolerant. Based on
BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated
by the genus Bacillus (97.5%) represented by B.
licheniformis (119), B. aerius (44), B.
sonorensis (33), B. subtilis (subsp.
spizizenii (2) and subsp. inaquosurum (6)),
B. amyloliquefaciens (subsp.
amyloliquefaciens (4) and subsp. plantarum (4)),
B. tequilensis (3), B. pumilus (3) and
Bacillus sp. (19). Only six isolates (2.5%) belonged to the
genus Aeribacillus represented by A. pallidus
(4) and Aeribacillus sp. (2). In this study, B.
aerius and B. tequilensis are described for the
first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total
strains exhibited high amylolytic, proteolytic or cellulolytic activity
respectively.
Chryseomonas luteola has only rarely been reported as a human bacterial pathogen. It has been shown that this organism in particular affects patients with health or indwelling disorders. Most reported cases showed septicemia, meningitis, endocarditis, or peritonitis. Two C. luteola infections observed in Morocco are described in the present study
The bacterial diversity occurring in traditional Moroccan soft white cheese, produced in eight different regions in Morocco, was studied. A total of 164 lactic acid bacteria were isolated, purified and identified by whole-cell protein fingerprinting and rep-PCR genomic fingerprinting. The majority of the strains belonged to the genera Lactobacillus, Lactococcus, Leuconostoc and Enterococcus. Sixteen species were identified: Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus paracasei, Lactobacillus brevis, Lactobacillus buchneri, Lactococcus lactis, Lactococcus garvieae, Lactococcus raffinolactis, Leuconostoc pseudomesenteroides, Leuconostoc mesenteroides, Leuconostoc citreum, Eterococcus durans, Enterococcus faecalis, Enterococcus faecium, Enterococcus saccharominimus and Streptococcus sp.
Four isolates, which were obtained from Belgian, Moroccan and Romanian dairy products, constituted a homogeneous but unidentified taxon after screening with whole-cell protein fingerprinting. Complete 16S rRNA gene sequence analysis classified representative strains in the genus Enterococcus. Highest sequence similarities of 98?6 and 98?0 % were obtained with the species Enterococcus sulfureus and Enterococcus saccharolyticus, respectively. Growth characteristics, biochemical features, tRNA intergenic length polymorphism analysis, DNA-DNA hybridization and DNA G+C contents of selected strains demonstrated that they represent a single, novel Enterococcus species. It differs phenotypically from other enterococci in characteristics commonly considered as typical of this genus: no growth in 6?5 % NaCl or 0?4 % sodium azide, and no acid production from a wide range of carbohydrates. The name Enterococcus saccharominimus sp. nov. is proposed for this novel species; the type strain (LMG 21727 T =CCM 7220 T ) was isolated from contaminated pasteurized cow's milk.
To study the biodiversity of halophilic bacteria in a protected wetland located in Loukkos (Northwest, Morocco), a total of 124 strains were recovered from sediment samples from a marsh and salterns. 120 isolates (98%) were found to be moderately halophilic bacteria; growing in salt ranges of 0.5 to 20%. Of 124 isolates, 102 were Gram-positive while 22 were Gram negative. All isolates were identified based on 16S rRNA gene phylogenetic analysis and characterized phenotypically and by screening for extracellular hydrolytic enzymes. The Gram-positive isolates were dominated by the genus Bacillus (89%) and the others were assigned to Jeotgalibacillus, Planococcus, Staphylococcus and Thalassobacillus. The Gram negative isolates were dominated by the genus Vibrio (41%) and the others were assigned to Halomonas, Psychrobacter, Marinobacterium, Pseudoalteromonas, Salinivibrio and Photobacterium. The growth of strains obtained under different physico-chemical conditions and the screening for hydrolytic enzymes showed a high diversity even within the same species.
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