Enterococcus saccharominimus sp. nov., from dairy products

Abstract

Four isolates, which were obtained from Belgian, Moroccan and Romanian dairy products, constituted a homogeneous but unidentified taxon after screening with whole-cell protein fingerprinting. Complete 16S rRNA gene sequence analysis classified representative strains in the genus Enterococcus. Highest sequence similarities of 98?6 and 98?0 % were obtained with the species Enterococcus sulfureus and Enterococcus saccharolyticus, respectively. Growth characteristics, biochemical features, tRNA intergenic length polymorphism analysis, DNA-DNA hybridization and DNA G+C contents of selected strains demonstrated that they represent a single, novel Enterococcus species. It differs phenotypically from other enterococci in characteristics commonly considered as typical of this genus: no growth in 6?5 % NaCl or 0?4 % sodium azide, and no acid production from a wide range of carbohydrates. The name Enterococcus saccharominimus sp. nov. is proposed for this novel species; the type strain (LMG 21727 T =CCM 7220 T ) was isolated from contaminated pasteurized cow's milk.

Application of multilocus sequence analysis (MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes

“…T (Vancanneyt et al, 2004), Enterococcus CDC PNS-E1 (=LMG 22681 T ) (Carvalho et al, 2004) and E. italicus LMG 22039…”

Enterococcus aquimarinus sp. nov., isolated from sea water

“…acetoin production, ribose acidification, resistance to 6?5 % NaCl and growth at 10 and 45 uC, are no longer applicable (Devriese et al, 1993;Devriese & Pot, 1995;Domig et al, 2003). Other methods, such as SDS-PAGE analysis of whole-cell proteins, have been used intensively as routine and validated identification systems for enterococci (Devriese et al, 2002;De Graef et al, 2003;Vancanneyt et al, 2004).…”

“…Degradation of the isolated DNA into nucleosides and their separation by HPLC was performed as described by Vancanneyt et al (2004). The DNA G+C content of strains LMG 16607 T and LMG 16612 was 38?7 mol%.…”

“…The taxonomic position of both sea-water isolates was further elucidated in this work. The reference strains used for comparison in this study were obtained from the BCCM/LMG bacteria collection (http://www.belspo.be/bccm/).To determine the phylogenetic position of the sea-water isolates, 16S rRNA gene sequence analysis was performed on one strain, LMG 16607 T , as described by Vancanneyt et al (2004). The sequence obtained (a continuous stretch of 1510 bp) was aligned with reference sequences obtained from GenBank and edited by using the BioEdit program (Hall, 1999) and ForCon (Raes & Van De Peer, 1999).…”

“…To determine the phylogenetic position of the sea-water isolates, 16S rRNA gene sequence analysis was performed on one strain, LMG 16607 T , as described by Vancanneyt et al (2004). The sequence obtained (a continuous stretch of 1510 bp) was aligned with reference sequences obtained from GenBank and edited by using the BioEdit program (Hall, 1999) and ForCon (Raes & Van De Peer, 1999 constructed using the neighbour-joining method with TREECON software (Van De Peer & De Wachter, 1994).…”