Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

Berrada, Imane; Willems, Anne; Vos, Paul De; Fahime, Elmostafa El; Swings, Jean; Bendaou, Najib; Melloul, Marouane; Amar, Mohamed

doi:10.5897/ajmr-11-1490

Cited by 13 publications

(25 citation statements)

References 38 publications

(9 reference statements)

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“…Kakhki et al (2011) reported that under their conditions Halorubrum is the predominant genus showing the highest rates of amylase, lipase, pullulanase, inulinase and DNase production. Our data is much more similar to the results reported by Berrada et al (2012), in which Bacillus is the predominant genus showing the higher production rates for amylase, lipase, DNase, protease and cellulase. It is interesting that higher average rates for production of the enzymes by a certain species do not necessarily mean that the higher enzyme-producing activity belonged to a strain from the same species.…”

Section: Enzymatic Profiles Of Speciessupporting

confidence: 91%

Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments

Wei¹,

Liu²,

Cui³

et al. 2018

Biosci. J.

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A total of 114 moderately halophilic bacteria were isolated from marine sediment environments. The isolates are belonged to 23 species based on the 16S rRNA sequence analysis. 63, 52, 47, 57, 74, 15 and 4 isolates are able to produce protease, amylase, lipase, pectinase, pulluanase, xylanase, cellulase, respectively. Combined hydrolytic enzyme activity analysis show that 15 strains present 1 hydrolytic activity, 32 strains present 2 hydrolytic activities, 21 strains present 3 hydrolytic activities, 26 strains present 4 hydrolytic activities, 11 strains present 5 hydrolytic activities and 2 strains present 6 hydrolytic activities. Hydrolase activities are widely distributed in a variety of species. The highest rates for production of protease, amylase, lipase, pectinase, pullanase, xylanase and cellulase were observed in species of B. baekryungensis, Hallobacillus sp., B. pumilus, B. megaterium or P. chungwhensis, B. amyloliquefaciens, B. pumilus, B. baekryungensis, respectively. However, the higher activities of protease, pectinase and pulluanase are frequently produced by the species of Halomonas sp. B. amyloliquefaciens or P. chungwhensis, and Vibrio sp. respectively. This investigation show that the diversity of halophilic bacteria from marine sediments could serve as a potential source of hydrolytic enzymes for industrial applications.

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Section: Enzymatic Profiles Of Speciessupporting

confidence: 91%

Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments

Wei¹,

Liu²,

Cui³

et al. 2018

Biosci. J.

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“…clones detected in stone monuments in Spain (Ettenauer et al ., 2011 ) and with cultured Bacillus sp. isolated from saline environments (Berrada et al ., 2012 ). In addition, sequences related to uncultured clones retrieved from skin microbiome (Grice et al ., 2009 ), as well as to the cultured Geomicrobium halophilum (Echigo et al ., 2010 ), were detected on sample To800 (18.7%).…”

Section: Resultsmentioning

confidence: 99%

“…It has been reported that halophilic bacteria have a role in damage occurring in brine-cured hides with their high proteoliytic activity (Bailey and Birbir, 1993 ). Many Bacillus species are known to produce alkaline and saline stable proteases, amylases, cellulases, lipases, pectinases and xylanases (Martins et al ., 2001 ; Berrada et al ., 2012 ).…”

Section: Resultsmentioning

confidence: 99%

Unmasking the measles‐like parchment discoloration: molecular and microanalytical approach

Piñar

Sterflinger

Pinzari

2014

Environmental Microbiology

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Many ancient parchments are defaced by red or purple maculae associated with localized destruction of collagen fibres. Although the main characteristics of this damage were present in most of the manuscripts analysed by many authors, no common microbial or fungal denominator has been found so far, and little or no correspondence between the microbial or fungal species isolated from materials could be addressed. In this study, culture-independent molecular methods and scanning electron microscopy (SEM) were used to identify fungal and bacterial communities on parchments affected by the purple stains. Protocols for c extraction and nucleic-acid-based strategies were selected for assays examining the community structure of fungi and bacteria on biodeteriorated parchment. Both SEM and molecular analysis detected the presence of bacterial and fungal cells in the damaged areas. Halophilic, halotolerant proteolytic bacterial species were selected by the saline environment provided by the parchment samples. As common microbial denominators, members of the Actinobacteria, mainly Saccharopolyspora spp. and species of Aspergillus, were detected in all investigated cases. It is proposed that a relationship exists between the phenomenon of purple spots on ancient parchments and that of the ‘red heat’ phenomenon, known to be present in some products manufactured with marine salt.

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“…Therefore, in this study, the standard 97% sequence identity with the closely related Bacillus was used to assign Bacillus isolates to the same species level. Based on this, extremely halotolerant Bacillus isolates screened from Dead Sea black mud were assigned to eight Bacillus species ( (Garabito et al, 1998;Palmisano et al, 2001;Roongsawang et al, 2002;Berrada et al, 2012;Zhang et al, 2012;Abbas et al, 2015;Dunlap et al, 2015) which demonstrated that those Bacillus species can tolerate increased salt concentrations. Whereas, the remained B. amyloliquefaciens was not previously defined as a halotolerant bacterium but Zar et al (2013) demonstrated that this bacterium has the ability to produce halotolerant enzymes.…”

Section: Discussionmentioning

confidence: 99%

Isolation and characterization of extremely halotolerant Bacillus species from Dead Sea black mud and determination of their antimicrobial and hydrolytic activities

Obeidat¹

2017

Afr. J. Microbiol. Res.

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This is the first study that investigated the isolation of extremely halotolerant Bacillus species from Dead Sea black mud. Nine isolates obtained from black mud were considered to be extremely halotolerant Bacillus based on morphological, physiological, and biochemical properties. Most of their colonies were white to light yellow and circular to irregular. All isolates were Gram-positive rod-shaped endospore-forming bacteria, facultative anaerobes, oxidase negative, catalase positive, mesophilic, extremely halotolerant, reacted positively for tryptophan deaminase and Voges-Proskauer, hydrolyzed gelatin and aesculin, and assimilated potassium gluconate. Most of the isolates were found to hydrolyze o-nitrophenyl-beta-D-galactoside (ONPG) and p-nitrophenyl-β-D-galactopyranoside (PNPG) as well as arginine, and assimilate D-mannose, N-acetylglucosamine, D-maltose, and malic acid. All isolates were considered to be nitrate reducers, six of them were nitrite producers and three were N 2 producers, suggesting that they may play an important role in nitrification-denitrification processes and in the nitrogen cycle in soil. Based on 16S rRNA gene sequence analysis, the isolates were found to share very high identities (97-99%) with their closest phylogenetic relative and they were assigned to eight Bacillus species (B. oceanisediminis, B. subtilis, B. firmus, B. paralicheniformis, B. methylotrophicus, B. amyloliquefaciens, B. sonorensis, and B. malikii). Interestingly, several enzymatic activities were detected from nonhemolytic isolates DSM2 and DSM7 that were identified as B. paralicheniformis. It was found that only DSM2 isolate produced promising antimicrobial activities. Its aqueous extract showed the highest significant antifungal activity. Whereas, n-butanol and methanol extracts showed significant antibacterial and antifungal activities against human skin pathogens and against other frequent human pathogens.

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Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

Cited by 13 publications

References 38 publications

Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments

Diversity of culturable moderately halophilic bacteria producing extracellular hydrolytic enzymes from marine sediments

Unmasking the measles‐like parchment discoloration: molecular and microanalytical approach

Isolation and characterization of extremely halotolerant Bacillus species from Dead Sea black mud and determination of their antimicrobial and hydrolytic activities

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