Many factors such as cryptorchidism, testicular torsion, varicocele, hyperthyroidism, infection, physical exertion, hormonal imbalance, the impact of xenobiotics induce the formation of reactive oxygen species (ROS) in the testis (Aitken and Roman, 2008). ROS is one of the principal causes of alteration in glucose homeostasis in testis, especially in terms of lactate production and modulation of LDH activity (Rossi et al., 2016) and apoptosis of germ cell (Rao and Shaha, 2000).Moringa oleifera (MO), a drumstick or horse-radish tree from Africa and India, possess high nutritional and research Article Abstract | Testicular toxicity is a significant cause of male infertility. It may occur due to hormonal, nutritional, behavioral, and environmental imbalance. Moringa oleifera, on the other hand, is known to have antioxidant, antiinflammatory, anti-tumor, anti-hypertensive, anti-diabetic properties. This study assessed the ameliorative effect of Moringa oleifera leaves extract (M/MOLE) on melamine-induced testicular toxicity in rats. Fifty Sprague Dawley (8weeks old) male rats were randomly assigned to group 1 (control), group 2 ( Moringa oleifera leaves extract only), group 3 (administered melamine only), and group 4 (both melamine and M/MOLE). Melamine significantly decreased (P < 0.05) semen quality, testicular weight, gonadal-somatic index, follicle-stimulating hormone (FSH), free testosterone, total testosterone, and an expression of steroidogenic enzyme genes (CYP11A1 and HSD17B3). A concurrent administration of M/MOLE reversed the clinical impact of Melamine treatment (P < 0.05). Histopathological examination revealed an improvement in testicular tissue injury in concurrent M/MOLE treatment groups than the melamine group. In conclusion, MOLE was effective in improving the toxic effects of melamine on testicular tissue in rats.