To fully understand the histological, morphometrical and heamodynamic variations of different supratesticular artery regions, 20 mature and healthy Assaf rams were examined through ultrasound and morphological studies. The testicular artery images of the spermatic cord as shown by B-mode analysis indicated a tortuous pattern along its course toward the testis, although it tends to be less tortuous close to the inguinal ring. Doppler velocimetric values showed a progressive decline in flow velocity, in addition to pulsatility and vessel resistivity when entering the testis, where there were significant differences in the Doppler indices and velocities among the different regions. The peak systolic velocity, pulsatility index and resistive index were higher in the proximal supratesticular artery region, followed by middle and distal ones, while the end diastolic velocity was higher in the distal supratesticular region. The total arterial blood flow and total arterial blood flow rate reported a progressive and significant increase along the testicular cord until entering the testis. Histological examination revealed presence of vasa vasorum in the tunica adventitia, with their diameter is higher in the proximal supratesticular zone than middle and distal ones. Morphometrically, the thickness of the supratesticular artery wall showed a significant decline downward toward the testis; meanwhile, the outer arterial diameter and inner luminal diameter displayed a significant increase distally. The expression of alpha smooth muscle actin and vimentin was higher in the tunica media of the proximal supratesticular artery zone than in middle and distal ones.
The frequency of semen collection is a crucial factor to consider in the rams performance inside breeding centers workout. To evaluate this factor, ram Breeding Soundness Evaluation could include sperm quality evaluation and new predictive and non-invasive tools such as ultrasound technique. In this work, an advanced ultrasonography technology, analyzing the testicular volume, echotexture, and vascular function, was used in three different frequencies of semen collection (abstinence frequency, AF; standard frequency, SF; and intensive frequency, IF). Semen samples were cooled (15°C, 6 h) and evaluated in terms of production, motility, viability, apoptosis, and content of reactive oxygen species. Correlation coefficients were calculated between ultrasonography measurements of echotexture and blood flow and sperm quality parameters. Our results showed an increase in the testicular echotexture when the frequency of semen collection was intensified. Doppler parameters (PSV, RI, PI, TABF) increased (P ≤ 0.05) when the frequency of semen collection was intensified. The sperm motility and functionality decreased in the samples of IF (P ≤ 0.05), evidencing the frequency of semen collection's influence. Moreover, moderate positive correlations were established among echotexture and different Doppler parameters with motility parameters in SF. Furthermore, the influence of abstinence days on AI success was analyzed in a field assay. The highest fertility rates were obtained when males had two to five abstinence days. To conclude, frequency of semen collection could be influenced in terms of semen quantity and sperm quality, showing changes in parenchyma echotexture and testicular vascularization. The standard semen collection frequency was the most adequate option. In addition, ultrasonography may be a predictive tool for estimating variations in the sperm quality of donor rams subjected to different frequencies of semen collection in reproduction centers.
This paper presents the results of studying the effect of carbon nanotubes on thermoelectric properties of
p-type (Nb0.6Ta0.4)0.8Ti0.2FeSb and n-type Ti0.5Zr0.25Hf0.25NiSn half Heusler alloys. The experimental data obtained indicate a strong effect of the carbon nanotubes on electrical conductivity and Seebeck coefficient of the n-type compound, while the changes in these properties in the p-type compound were significantly less. It is suggested that a possible reason for this difference is the formation of a conducting cluster of carbon nanotubes in the sample of the n-type Heusler alloy.
The aim of this paper was to study the effectiveness of N 2 /Ar gas ratio on the mechanical and tribological properties of Ti-Zr-N films coated on (AISI 304 stainless steel) substrate. The thin films were produced at 150 W pulsed magnetron sputtering (PMS) technique operated for 90 min. The structure and tribo-mechanical properties of the Ti-Zr-N coatings were investigated at different N 2 /Ar gas pressure ratio. X-ray diffraction (XRD), microhardness, surface roughness, wear and friction, corrosion performance and surface energy were investigated. The analysis of X-ray diffraction (XRD) indicated a formation of solid solution phase of (Ti-Zr)N with (FCC) structure and a chemical compound phase of TiN. The solid solution phase (Ti-Zr)N has the orientation (111). The microhardness of the thin films has high values compared to associated value of (AISI 304 stainless steel) substrate; up to 4-folds. Furthermore, the wear performance was improved from 73.06 mm 3 /m for AISI substrate to 5.01 mm 3 /m after depositing Ti-Zr-N. The coefficient of friction was decreased from nearly 0.69 for the austenitic substrate to nearly 0.12 after coating with Ti-Zr-N. Over and above, the corrosion resistance substantially improved (factor of 1000 times) after the coating.
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