The assembly of an inclusion complex in an aqueous medium
using
a metabolizer drug (dyphylline) as guest and β-cyclodextrin
as host has been established, which is extremely appropriate for a
variety of applications in modern biomedical sciences. The formation
of the inclusion complex is established by
1
H NMR, and
surface tension and conductivity measurements demonstrate that the
inclusion complex was produced with 1:1 stoichiometry. The thermodynamic
parameters based on density, viscosity, and refractive index measurements
were used to determine the nature of the complex. This research also
forecasts how dyphylline will release in the presence of CT-DNA without
any chemical modifications. The produced insertion complex (IC) has
a higher photostability due to the drug dyphylline being protected
by β-CD. The antibacterial activity of dyphylline greatly improved
after complexation and exhibited higher toxicity against Gram-negative
(highest against
Escherichia coli
)
in comparison to Gram-positive bacteria. The encapsulation mode of
the dyphylline molecule into the cavity of the β-CD was also
investigated using DFT to confirm preliminary results.
The myth of inactivity of inorganic materials in a biological system breaks down by the discovery of nanozymes. From this time, the nanozyme has attracted huge attention for its high durability, cost-effective production, and easy storage over the natural enzyme. Moreover, the multienzyme-mimicking activity of nanozymes can regulate the level of reactive oxygen species (ROS) in an intercellular system. ROS can be generated by peroxidase (POD), oxidase (OD), and Fenton-like catalytic reaction by a nanozyme which kills the cancer cells by oxidative stress; therefore, it is important in CDT (chemo dynamic therapy). Our current study designed to investigate the enzyme mimicking behavior and anticancer ability of cerium-based nanomaterials because the cerium-based materials offer a high redox ability while maintaining nontoxicity and high stability. Our group synthesized CeZrO 4 nanoparticles by a green method using β-cyclodextrin as a stabilizer and neem leaf extract as a reducing agent, exhibiting POD-and OD-like dual enzyme activities. The best enzyme catalytic activity is shown in pH = 4, indicating the high ROS generation in an acidic medium (tumor microenvironment) which is also supported by the Fenton-like behavior of CeZrO 4 nanoparticles. Inspired by the high ROS generation in vitro method, we investigated the disruption of human kidney cells by this nanoparticle, successfully verified by the MTT assay. The harmful effect of ROS in a normal cell is also investigated by the in vitro MTT assay. The results suggested that the appreciable anticancer activity with minimal side effects by this synthesized nanomaterial.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.