El objetivo del estudio fue determinar la existencia de subpoblaciones espermáticas según la morfometría de los espermatozoides de llama, utilizando un sistema CASA-Morph. Se colectó semen de cuatro llamas Q’ara de 4-6 años mediante el método de electroeyaculación en cuatro oportunidades por animal, con intervalos de una semana. Se determinó el volumen, movilidad total, concentración de espermatozoides, porcentaje de espermatozoides vivos y porcentaje de espermatozoides con reacción acrosomal. Los parámetros morfométricos de los espermatozoides se evaluaron utilizando el sistema CASA-Morph, Integrated Semen Analysis System (ISAS®v1). Se determinó la longitud, anchura, área, perímetro, elipticidad, elongación, regularidad y rugosidad de la cabeza del espermatozoide de llama, así como la anchura, área, distancia y ángulo de inserción de la pieza intermedia del espermatozoide. Las variables morfométricas se distribuyeron en cinco componentes principales (PCA) denominados elongación, longitud, circularidad, anchura de la pieza intermedia e inserción de la pieza intermedia, que explicaron un 81.6% de la varianza total. El análisis de clústeres determinó cuatro subpoblaciones (SP): SP1 agrupó células pequeñas con baja elongación y elipticidad (36.8%); SP2 de espermas de tamaño intermedio, tanto en la longitud y anchura de la cabeza (9.9%); SP3 de células alargadas con valores bajos de anchura de la cabeza y pieza intermedia (33.0%); y SP4 de espermatozoides con una anchura de la pieza intermedia alta (20.2%). Se hallaron diferencias significativas de subpoblaciones intra e inter animal.
In recent years, the study of male factor in conjugal infertility, has received more attention since it was recognized as an important cause of infertility in 30-50% of the cases; and to the diminishing quality of semen, observed during the last decades, possibly related to environmental polution.Alterations in sperm motility has been included as a direct cause of male infertility along with the traditionally known causes. Frequently these alterations have been related to morphological changes of spermatozoa. The morphology and size of sperm cells are higly variable in human semen and abnormal forms also can be found in fertile men. Experimental data demonstrate that abnormal spermatozoa are not functional cells. There are many clinical reports that associate spermatic dismorphy with infertility. Previously reported abnormalities in the sperm morphology related to infertility include: acrosomal agenesis, defects in the dynein arms and fibrous sheath displasia. Therefore, it is important to undertake the ultrastructural study of spermatozoa to determine subcellular pathology of the gamete which could explain the reproductive failure, specially if there is no evident cause of the motility defect or if there has been no response to treatment, or if the assisted reproductive programs have failed repeatedly.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.