Background and Aim. Wound healing is a complicated physiological process to preserve skin integrity after injuries and includes the proliferative phase, hemostasis/inflammatory phase, and remodeling through extracellular, intercellular, and intracellular components synchronization. The study aimed to examine human placental collagen/ibuprofen’s effect on wound healing in an animal model. Materials and Methods. The cell viability test was performed by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. In this animal study, three circular excisions were made in the back of each of the 12 rats and injected with the following groups: collagen/ibuprofen, group 1; collagen, group 2; and unfilled as the control group, group 3 for two times. The healing procedures were explored via calculating wound contraction percentage after taking photographs on days 7 and 14. Also, histopathological assessments were conducted on days 7 and 14. Results. The MTT results showed no significant cytotoxicity, and macroscopic results demonstrated that the percentage of wound contraction was 85%, 79%, and 41% in the collagen/ibuprofen, collagen, and control groups on 14th day, respectively. Histopathological assessments showed that hair follicles number, fibroblasts content, and angiogenesis in the collagen/ibuprofen were remarkably more significant than collagen and control groups. Conclusion. The collagen/ibuprofen group had significant outcomes compared with collagen and the control groups in wound healing and wound contraction. Collagen/ibuprofen hydrogel can be a potential hydrogel in accelerating wound healing.

Necrotizing sarcoid granulomatosis (NSG) is a rare granulomatous pneumonitis which is composed of a background of sarcoidosis-like granulomas, granulomatous vasculitis, and variable amount of necrosis. We reported a case of a 38-year-old nonsmoking woman presented with left-sided chest pain and dyspnea for three days. Chest CT scan exhibited collapse consolidation of the left lower lobe with the presence of two separated small-sized cystic lesions within the collapsed segment. Lung lesion resection was performed, and histological examination confirmed the diagnosis by excluding other causes of granulomatous diseases. The prognosis of NSG is favorable, and medical treatment is usually not necessary, as well as in our case. NSG is a rare disease with nonspecific symptoms and good prognosis which is frequently confused with Wegener’s granulomatosis, sarcoidosis, and Churg–Strauss syndrome. This entity should also be considered as differential diagnosis of necrotizing granulomatous diseases.

Background: One of the most common gastrointestinal surgeries is cholecystectomy. Gallstones are the major causes of cholecystectomy and induce various histopathologic changes. Gallbladder carcinoma is rare with poor prognosis. Metaplasic changes in gallbladder epithelium are considered precancerous lesions. Objectives: This study aimed at revealing the spectrum of histopathological patterns of gallbladder diseases in cholecystectomy specimens. Methods: A total of 1004 gallbladder H & E stained slides of cholecystectomy specimens over one year were studied. Histopathological evaluation was done by 6 pathologists in terms of variables. Results: In our study, the male: female ratio was 1:2.1. The age of patients varied from 17 to 96 years with a mean age of 49.1 years. The mean age of patients with gallstones was 47.52 years and was more common in women. Cholesterol, pigmented, and mixed stones were reported in 58.36%, 39.03%, and 2.6% of gallstones, respectively. The most common histological morphology was chronic calculous cholecystitis (61.18%). The incidence of metaplasia and invasive carcinoma was 19.55% and 0.2%, respectively. Conclusions: In this study, histopathological analysis of gallbladder diseases in the cholecystectomy specimens revealed that chronic calculous cholecystitis was the most common histologic finding. Gallstones were found more commonly in women and cholesterol stone was the predominant type. Pyloric metaplasia was the most frequent type of metaplasia. The rate of invasive carcinoma is compatible with those reported in the literature. Routine histopathological analysis of the cholecystectomy specimens will help to detect incidental carcinoma and precursors of malignancy.

BACKGROUND: Renal cell carcinoma is one of the most common tumors of the kidney. Glucose transporters, transport glucose, and increased expression of these transporters have been reported in various tumor types. Glucose transporter 1 (GLUT1), the best-known glucose transporter, has an important role at several stages in cancer progression. The overexpression of GLUT1 in the tumor cells indicates an increased proliferation and invasive behavior of the tumor. AIM: This study aims to investigate the expression of GLUT1 in renal cell carcinoma and its subtypes. METHODS: This study is a descriptive cross-sectional study that was performed on patients with renal cell carcinoma. Seventy reports of formalin fixed; paraffin-embedded blocks of renal cell carcinoma were selected from pathology archives. The samples included: clear cell type renal cell carcinoma, RCC clear cell type with sarcomatoid feature, papillary renal cell carcinoma, and chromophobe renal cell carcinoma. RESULTS: In this study, 50 male and 20 female samples (71.4% and 28.6%) with the mean age of 57.9 ± 13.1 years were studied. Forty-three samples (61.4%) were positive for GLUT1 and 27 (38.9%) were negative for it. For the GLUT1 expression being positive or negative between the two groups, was not significantly affected by the age, sex, and the grade of the tumor, </AQ17>while the difference between the two groups was statistically significant in terms of stage and type of tumor (p < 0/001, p = 0.039). CONCLUSION: Renal cell carcinoma of ccRCC type is associated with increased GLUT1 expression. Therefore, the GLUT1 immunohistochemistry marker can be a useful marker for diagnosis of RCC, specifically ccRCC type.

Cutaneous lesions in immunocompromised patients with positive results in acid-fast staining and negative results for M. tuberculosis should be further assessed using skin culture and molecular techniques to identify rare, atypical mycobacterial species like M. haemophilum.