The main objective of this study was to analyse the occurrence of yeasts and filamentous fungi in drinking water

Thiosemicarbazides are compounds known for their biological activity, particularly their antimicrobial properties, which include activity against fungi. The difficulty of treating fungal diseases induced us to assess the antifungal properties of some novel thiosemicarbazide compounds. We selected the natural products limonene and camphene as sources for the preparation of these new thiosemicarbazide derivatives. The compound N(4)-[2,2-dimethyl-3-methylnorbornane]-thiosemicarbazide (TIO C) showed an antifungal effect on Trichophyton mentagrophytes, with values of MIC = 55 μmol L-1 and MFC = 110 μmol L-1. Scanning-electron microscopy showed a decrease in mycelium development and morphological alterations of T. mentagrophytes cultured on nail fragments and treated with TIO C. In an attempt to discover its mode of action, we noted that ergosterol is apparently not a target of TIO C activity. An effect of TIO C on T. mentagrophytes cell walls and dividing cross-walls was shown by observed impairment of the fluorescence of tissues stained with calcofluor white, a specific marker for fungal chitin, suggesting that the compound can affect and damage the cell-wall structure or may interfere with its formation, during cell division, growth, and morphogenesis. This approach to the synthesis of new derivatives might provide interesting compounds with greater biological activity in pharmacological research.

Ocotea odorifera is a medicinal plant that is popularly known in Brazil as "canela-sassafrás" and is used to treat dermatosis. This study investigated the antifungal properties of O. odorifera. The methanol extract of O. odorifera was submitted to successive chromatographic separation and yielded Tellimagrandin II (TEL). Candida parapsilosis strain ATCC 22019 was used to determine the minimum inhibitory (MIC) and fungicidal concentrations, and to study the synergistic action with nystatin (NYS), amphotericin (AMP), and fluconazole (FLU). After treatment, the morphology of the yeast was analysed by scanning electron microscopy. Cytotoxicity was assessed in Vero cells, and genotoxicity by the micronucleus test. The TEL structure was proposed based on NMR and comparison with literature data and ESI-MSMS analysis. The compound showed potent inhibitory activity against C. parapsilosis, with a MIC of 1.6 μM. TEL acted synergistically with NYS, AMP, and FLU, and caused morphological alterations in the yeast cells. The methanolic extract showed low cytotoxicity in vitro and in vivo, and was not mutagenic in mice (P < 0.05). The use of O. odorifera in traditional medicine seems to have a valid basis, in view of the antifungal activity of TEL demonstrated in this study, and may contribute to potential drug development.

IDENTIFICATION OF MATERNAL NEAR MISS IN AN INTENSIVE CARE UNITABSTRACT: Cases of maternal near miss were identified and health indexes were listed in hospitalized patients in ICUs. Current transversal and retrospective analysis was performed at the 15 th Regional Health Unit in Maringá PR Brazil. There were 37 hospitalizations, of which 16 were near miss cases and 3 involved death of the mother. Main complications were hypertension (45%), lung condition (31%) and hemorrhage (24%). The most common invasive procedure comprised transfusion of red blood cells in 51% of cases. Mothers´ fatal cases had the greatest number of near miss criteria. The identification of maternal near miss cases and mother´s death may better monitor obstetric care and may be a useful tool for teams dealing with maternal mortality.

The research in sustainable development goals (SDG) increases year by year since its approval in 2015. Typically, after a phase of exponential growth, the number of publications increases at lower rates, suggesting a consolidation process in which literature reviews become a relevant and high-evidence type of document. In this context, the aim of this study was to perform an unprecedented bibliometric analysis of literature reviews on SDG to assess the evolution and consolidation of the scientific research. Article reviews on SDG from 2015 to 2022 were retrieved from Web of Science core collection and a descriptive bibliometric analysis was performed by growth rate, research area, source, citation, and region. Mapping and cluster analysis using keyword co-occurrence, co-authorship, and bibliographic coupling were also applied. The result revealed that SDG is a fast-growing field, with a trend in the diversification of research areas. Most of the review documents were categorized in general aspects of sustainability. Technology (SDG 9) and economic growth (SDG 8) were spotted as hidden key research areas. This result is contrary to previous bibliometric studies on SDG, demonstrating the rapid evolution and change in the field. In addition, literature reviews on reduced inequalities (SDG 10), gender equality (SDG 5); oceans, seas, and marine environments (SDG 14); and peace, justice, and strong institutions (SDG 16) were revealed as research gaps. Thus, the results demonstrated that the research on SDG cannot yet be considered a consolidated area of research, as it leaves many SDG unexplored. Future research has been proposed accordingly.

Seafood/fish intake has been regarded as a protective factor for coronary heart disease (CHD), while smoking is a strong risk factor. To examine whether associations between smoking and risk of CHD are modified by seafood/fish intake, we studied 72,012 Japanese men and women aged 45-74 years who completed 2 food frequency questionnaires, 5 years apart, during the period 1995-2009. After 878,163 person-years of follow-up, 584 incident cases of CHD (101 fatal and 483 nonfatal), including 516 myocardial infarctions, were documented. There was a clear dose-response association between smoking and CHD risk among subjects with a low seafood/fish intake (<86 g/day) but not among those with a high seafood/fish intake (≥86 g/day). Compared with never smokers, the multivariable hazard ratios in light (1-19 cigarettes/day), moderate (20-29 cigarettes/day), and heavy (≥30 cigarettes/day) smokers were 2.39 (95% confidence interval (CI): 1.60, 3.56), 2.74 (95% CI: 1.90, 3.95), and 3.24 (95% CI: 2.12, 4.95), respectively, among low seafood/fish eaters and 1.13 (95% CI: 0.64, 1.99), 1.29 (95% CI: 0.95, 2.04), and 2.00 (95% CI: 1.18, 3.51), respectively, among high seafood/fish eaters. Compared with heavy smokers with a low seafood/fish intake, light smokers with a high seafood/fish intake had substantially reduced risk of CHD (hazard ratio = 0.57, 95% CI: 0.32, 0.98). High seafood/fish intake attenuated the positive association between smoking and risk of CHD.

No abstract

O estudo objetivou comparar o grau de satisfação profissional entre trabalhadores de Enfermagem de uma instituição pública e aqueles que atuam em um serviço privado de saúde mental. Realizou-se pesquisa transversal, descritiva e de abordagem quantitativa em dois serviços psiquiátricos (um público e outro privado). No total, 70 profissionais de enfermagem fizeram parte da pesquisa. A coleta de dados ocorreu entre abril e maio de 2013, aplicando-se um instrumento de caracterização. E para avaliar a satisfação dos profissionais foi aplicado o instrumento SATIS-BR. Os resultados foram analisados com auxílio do software estatístico R, admitindo-se como significativo p-valor <0,05. Os profissionais do serviço privado representaram 70% (n=49) dos sujeitos; porém, independentemente da instituição, a maioria dos participantes era do sexo feminino e apresentava grau de instrução de nível médio/técnico. Quanto à satisfação laboral, os profissionais do serviço público apresentaram escore médio global de 3,53 pontos e a equipe do serviço privado escore médio de 3, 18 pontos, sendo a diferença estatisticamente significativa (p-valor <0,001). Assim, constatou-se que, na análise global, a equipe de enfermagem trabalhadora na instituição pública mostrou-se mais satisfeita com o serviço.