The aim of the present study is to explore the use of salivary 8-hydroxideoxyguanosine (8-OHdG) and Interleukin-1 (IL-1) gene polymorphism in the diagnosis of the patients with aggressive periodontitis. The correlation between salivary 8-OHdG level and clinical parameters was analyzed, at the same time as the use of 8-OHdG level and IL-1gene polymorphism in patients with aggressive periodontitis. Eighteen patients suffering from aggressive periodontitis and 18 healthy subjects without any sign of periodontitis were enrolled into the study after clinical examination. The analysis of genetic polymorphism of IL-1 gene was carried out from oral swabs by using the GenoType IL-1 test; the 8-OHdG biomarker was quantified from saliva samples by using an ELISA competition test. The salivary level of 8-OHdG in the control group was 0.70±0.54 ng/mL and in aggressive periodontitis, 6.93±2.90 ng/mL (p<0.001). A positive genotype consisting of allele 2 (Thymine/Thymine) was found with lower prevalence in healthy subjects-5.56%-when compared to aggressive periodontitis, respectively 72.22 % (p<0.001). Our study demonstrated that the salivary level of the 8-OHdG biomarker and IL-1 gene polymorphism can be used in the evaluation of the oro-dental status at patients with aggressive periodontitis.
L'interleukine1 beta-un marqueur de surveillance de l'evolution des reconstitutions prothetiques fixes Introduction. Le but de la médecine dentaire moderne est la restauration du système bucco-dentaire du patient, en renforçant sa santé, des points de vue fonction, confort et esthétique. Méthodes. Le groupe d'étude était composé de 30 patients chez lesquels ont été insérés des implants dentaires, et qui ensuite ont bénéficié de la réhabilitation orale par sur-prothèse comme suit: 18 patients avec prothèses fixes en métal-céramique (12 femmes, 6 hommes) et 12 patients avec prothèses en zirconium-céramique (5 femmes, 7 hommes). Chez tous les patients on a quantifié l'interleukine 1 bêta (IL-1) après l'insertion de l'implant à 7, 30 et 90 jours, et après leur surcharge à 0, 30 et 90 jours. Résultats. Les valeurs de l'IL-1étaient corrélées avec la profondeur du sillon péri-implantaire dans tous les 3 moments de détermination après l'insertion de l'implant (r>0,600), avec une signification statistique élevée entre les mensurations effectuées à 30 et 90 jours (p<0,001). Après la surcharge il a été démontré qu'il existe une corrélation entre les quantités de IL-1 et la profondeur du sillon péri-implantaire dans tous les moments d'évaluation (métal-céramique: r entre 0.774-0.871; zirconium-céramique: r entre 0.679-0.895).
Partial and total edentation has been a real problem worldwide and at all times. The realization of an individualized treatment plan for each form of edentation takes into account the particularities of the edentulous prosthetic field and the materials used as well as the conventional or modern techniques applied. The study group consisted of 220 patients in whom dental implants were inserted; the control group was randomized from 10 patients with a favorable evolution out of the 210 (94.55%). At 7 and 60 days after the insertion of the dental implants and 6 months after their prosthetic loading, crevicular fluid and peri-implant fluid was harvested, for the quantitative determination of Osteoprotegerin (OPG) and Interleukin 1β (IL1-β). Of the 220 patients studied, 10 developed peri implantitis (5.45%) as follows: 4 patients with mucositis and 6 patients with severe form. The results obtained show that there are differences with statistical significance between the OPG values obtained in crevicular fluid in healthy compared to patients with mucositis after 7 days (p <0.001). Regarding IL1-β, there are differences with high statistical significance between the levels in healthy patients and those with peri-implantitis after 7 days (p <0.001). Our results show the existence of a high correlation between the clinical status and these two parameters, especially after the determinations performed at 7 and 60 days. In conclusions, the present study shows that the OPG and IL1-β can be considered useful markers in the evaluation of the patient after the insertion of the dental implant and after its prosthetic loading.
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