In the present research, ethanolic and methanolic extracts of Teucrium polium (germander) were studied regarding the content of biologically active substances - in particular polyphenols - antioxidant activity and antibacterial activity. The extracts have been found to be rich in polyphenolic compounds, kaempferol and epichatechin have been found to be present in the highest concentrations. The antioxidant activity evaluated by the method of capturing the free radicals with DPPH revealed values close to those produced by the ascorbic acid. Antimicrobial activity was evaluated by the diffusimetric method and S. aureus and E. coli microorganisms were found to be the most sensitive while P. aeruginosa and fungal species were not sensitive.
In the last decade, the incidence of skin cancer has increased significantly and there is data that rank it as the most common form of malignancy in humans. Among the classified skin cancers, melanoma is the most severe with a significant mortality rate. The determining factor in the occurrence of skin cancer is generally recognised to be ultraviolet radiation. Skin pigmentation has been shown to provide a high degree of protection and this is due to melanin. In order to be able to consider certain hypotheses and to develop new approaches to melanoma, in this study it was studied the melanin content in certain cell types that have a direct connection with melanoma. The cell lines used in the study were: (i) human melanoma cell lines: A375, SK-MEL-1, SK-MEL-3, SK-MEL-5, SK-MEL-28, SH-4, and COLO 829; (ii) mouse melanoma cell lines: B164A5, B16F0, and B16F10; and (iii) healthy cell lines of human origin: HEMa (primary melanocytes), HaCaT (immortalised keratinocytes) and 1BR3 (human fibroblasts). The results obtained pointed out that A375, SK-MEL-28 and SK-MEL-5 cells do not produce melanin, SK-MEL-1, SH-4 and SK-MEL-3 cells produce melanin while COLO 829 cells � only in small passages produce melanin. Of the 3 lines of murine melanoma tested, the one that produces melanin in a higher concentration is B16F10, followed by B16F0 and B164A5.
Melanoma is a malignant disease of the cutaneous organ that shows significant aggression, resistance to treatment and increased mortality. Melanin and B-type ultraviolet radiation are key factors in the appearance and development of melanoma. In this study, the melanocyte and two melanoma tumor cells behaviour was evaluated in the presence of UVB radiation. The results highlighted the significant impairment of melanin-producing malignant cells, COLO-829, at both radiation doses tested.
As prophylactic and therapeutic approaches for melanoma, of great interest and importance are the in vitro studies using cell lines to elucidate several tumoral phenomena. Therefore, the similarities and differences between the different tumor cells must be known and understood in order to obtain a more accurate correlation with processes that occur in vivo. In this study, six cell lines of melanoma, both of mouse and human origin were analyzed from the point of view of cell culture growth, morphology and use in the research of new therapies. In brief, the current paper exhibits a comparison of melanoma cells which can be utilized as a starting point for further in vitro studies and in vivo animal models.
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