Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma

Bratu, Tiberiu; Berceanu, Mircea; Coricovac, Dorina; Crăiniceanu, Zorin; Gyori, Zsolt; Roman, Andreea; Marcovici, Iasmina; Dehelean, Cristina; Pînzaru, Iulia

doi:10.37358/rc.20.4.8084

Cited by 5 publications

(4 citation statements)

References 14 publications

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“…The two melanoma cell lines were selected based on the results of one of our previous studies indicating that B16-F10 cells contain the highest amount of melanin (56.452 µg/mL), while SK-MEL-5 cells lack pigmentation [29]. The results are expressed as cell viability percentage (%) normalized to control (unstimulated) cells.…”

Section: Resultsmentioning

confidence: 99%

In Vitro Insights Regarding the Role of Melanin in Melanoma Cells’ Response to Betulinic Acid Treatment

MELANIA¹

2022

FARMACIA

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Malignant melanoma (MM), the cancer arising from the normal-to-malignant switch of melanocytes, remains the most aggressive type of skin cancer with an annual increasing incidence worldwide. Based on the presence or absence of melanin, MM is divided in melanotic (pigmented) or amelanotic (non-pigmented) melanoma. Melanin is the natural pigment providing a protective role against harmful ultraviolet (UV) radiations and oxidative stress to epidermal melanocytes and surrounding keratinocytes. Recent studies reveal the ability of melanin to bind drugs via covalent and non-covalent bonds, leading to their accumulation within pigmented tissues which might influence the response of MM to chemotherapy. Betulinic acid (BA) is a pentacyclic triterpenoid possessing a multitude of therapeutic properties, including antimelanoma activity. The present study aimed to investigate the response of two MM cell lines to the antitumour effect of BA, considering their melanin content and to evaluate the cytotoxic potential of BA in healthy primary fibroblasts. The results reveal a dose-dependent decrease in the viability and confluence of all cell lines. However, SK-MEL-5 cells (IC50 =13.67 µM for 24 h, and 7.48 µM for 72 h) exerted a higher sensitivity to BA when compared to B16-F10 cells (IC50 = 52.12 µM for 24 h, and 25.54 for 72 h). BA induces a differential cytotoxicity in healthy, MM non-pigmented SK-MEL-5 and pigmented B16-F10 cells. Therefore, further studies are required to confirm the interaction between BA and melanin. RezumatMelanomul malign (MM), cancerul care rezultă din transformarea malignă a melanocitelor, rămâne cel mai agresiv tip de cancer cutanat, având o incidență anuală aflată în continuă creștere. În funcție de prezența sau absența melaninei, MM se clasifică în melanom melanotic (pigmentat) și amelanotic (nepigmentat). Melanina reprezintă pigmentul natural care oferă protecție melanocitelor și keratinocitelor epidermice împotriva radiațiilor ultraviolete (UV) și a stresului oxidativ. Studiile recente au evidențiat abilitatea melaninei de a lega medicamente prin legături covalente și non-covalente, rezultând acumularea lor la nivelul țesuturilor pigmentate, fenomen care poate influența răspunsul MM la chimioterapie. Acidul betulinic (BA) este un fitocompus din clasa triterpenelor pentaciclice care posedă o multitudine de proprietăți terapeutice, inclusiv activitate antimelanom. Acest studiu a urmărit investigarea răspunsului a două linii celulare de MM la efectul antitumoral al BA, având în vedere conținutul de melanină și evaluarea potențialului citotoxic al BA asupra unor celule sănătoase -fibroblaste primare. Rezultatele au arătat o scădere doză-dependentă în viabilitatea și confluența tuturor liniilor celulare. Totuși, linia celulară SK-MEL-5 (IC50 =13,67 µM pentru 24 h și 7,48 µM pentru 72 h) a manifestat o sensibilitate mai ridicată la acțiunea BA comparativ cu linia celulară B16-F10 (IC50 = 52,12 µM pentru 24 h și 25,54 pentru 72 h). BA induce o citotoxicitate dependentă de tipul de celule, celule sănătoas...

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Section: Resultsmentioning

confidence: 99%

In Vitro Insights Regarding the Role of Melanin in Melanoma Cells’ Response to Betulinic Acid Treatment

MELANIA¹

2022

FARMACIA

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“…By contrast, SK-MEL-28 cells are amelanotic cells presenting a melanocyte-like phenotype with polygonal morphology, capable to form large round cell type melanoma in vivo. These cells express as mutant genes BRAF, TP53, and CDK4 [ 35 ] and cannot produce melanin in vitro as shown in our previous study [ 36 ]. Recently, it has been highlighted that melanin plays a key role in the protection of pigmented cells against the injuries induced by chemical toxicants [ 37 ].…”

Section: Discussionmentioning

confidence: 95%

Rutin Exerts Cytotoxic and Senescence-Inducing Properties in Human Melanoma Cells

Pînzaru

Chioibaş

Marcovici

et al. 2021

Toxics

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Malignant melanoma represents the deadliest type of skin cancer with narrow treatment options in advanced stages. Herbal constituents possessing anticancer properties occupy a particular spot in melanoma research as potential chemotherapeutics. Rutin (RUT) is a natural compound exerting antioxidant, antimicrobial, anti-inflammatory, UV-filtering, and SPF-enhancing activities that are beneficial to the skin; however, its effect as an anti-melanoma agent is less investigated. The current study is focused on assessing the cytotoxic potential of RUT against two different human melanoma cell lines: RPMI-7951 and SK-MEL-28 by evaluating its impact in terms of cell viability, cells’ morphology, and nuclear aspect assessment, and senescence-inducing properties. The results indicate a dose-dependent decrease in the viability of both cell lines, with calculated IC50 values of 64.49 ± 13.27 μM for RPMI-7951 cells and 47.44 ± 2.41 μM for SK-MEL-28, respectively, accompanied by a visible reduction in the cell confluency and apoptotic features within the cell nuclei. RUT exerted a senescence-inducing property highlighted by the elevated expression of senescent-associated beta-galactosidase (SA-β-gal) in SK-MEL-28 cells. Despite the in vitro anti-melanoma effect revealed by our results, further studies are required to elucidate the mechanisms of RUT-induced cytotoxicity and senescence in melanoma cells.

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“…They were then fed for 7 days, and their ascites were extracted and diluted to 2 × 10 7 cells/mL. Another 50 mice were injected with 0.2 mL in their right forelimb; 40 mice were chosen for subsequent experiments when the tumor reached 50 m 3 (Berceanu et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Oxyepiberberine from the Active Site of Coptidis Rhizoma Inhibits Lung Adenocarcinoma by Regulating SRC-related Pathways in vivo

Zhang,

Xue,

Zeng

et al. 2024

Pharmacognosy Magazine

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Background: Huanglian (HL), also known as Coptis chinensis Franch., was initially documented in “Sheng Nong’s Herbal Classic” as a high-quality product used to cure ailments such as fever, indigestion, bloating, nausea, dysentery, diarrhea, abdominal pain, and tuberculosis. Nowadays, it is extensively employed in clinical settings to alleviate pulmonary diseases, like tuberculosis, lobular lung disease, and pulmonary abscess, and is worth exploring further. Nevertheless, there is limited research on the precise advancements of anti-lung adenocarcinoma (LUAD). Objectives: Using biological experiments and network pharmacology, the objective of this research was to investigate the most effective parts and chemical constituents of C. chinensis against LUAD and ascertain the probable targets and pathways. Materials and Methods: First, an MTT experiment verified the impact of each part on A549 and BEAS-2B cells. High performance liquid chromatography (HPLC) was then used to identify the chemical components of the effective substance in C. chinensis against LUAD. Then, network pharmacology was used to screen potential pathways and binding target proteins. Finally, a subcutaneous tumor model was established in mice using LLC cells to observe the effects of the effective components of C. chinensis against LUAD in tumor-bearing mice. Results: The MTT assay demonstrated the efficacy of oxyepiberberine (OPB) as an anti-LUAD compound with minimal toxicity and side effects. According to the results of network pharmacology and molecular docking, SRC appears to be the primary target of C. chinensis active substance against LUAD. In vivo experiments revealed that the OPB group exhibited lowered toxicity and side effects. Additionally, the density of tumor cells decreased and the nuclei were destroyed in OPB groups of tumor tissues. Finally, OPB could regulate apoptosis, migration, and protein expression significantly related to the SRC pathway in tumor cells. Conclusion: Our research reveals the anti-LUAD properties of C. chinensis through a combination of experimentation and network analysis. These findings establish a theoretical foundation for C. chinensis as a clinical antitumor agent. Furthermore, our work provided an essential groundwork for further investigations into the medicinal properties of C. chinensis.

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Melanin Quantification by Chemical Methods in Healthy and Melanoma Cell Lines Correlated with the Importance in the Development of Melanoma

Cited by 5 publications

References 14 publications

In Vitro Insights Regarding the Role of Melanin in Melanoma Cells’ Response to Betulinic Acid Treatment

In Vitro Insights Regarding the Role of Melanin in Melanoma Cells’ Response to Betulinic Acid Treatment

Rutin Exerts Cytotoxic and Senescence-Inducing Properties in Human Melanoma Cells

Oxyepiberberine from the Active Site of Coptidis Rhizoma Inhibits Lung Adenocarcinoma by Regulating SRC-related Pathways in vivo

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