Many studies have been conducted in order to define the root canal morphology based on age, gender, ethnic and racial characteristics of the population. However, relations within morphological variations of certain groups of teeth have rarely been investigated. The aim of this study was to evaluate the root canal morphology of mandibular lateral incisors and to determine the association between their morphologic characteristics and the presence of a second mesiobuccal canal of maxillary first molars. Cone-beam computed tomography images from a pre-existing base were analyzed for the bilateral presence of both permanent mandibular lateral incisors and permanent maxillary first molars. Root canal morphology was analyzed according to the Vertucci classification. Associations of root canal morphologies between incisors and molars were calculated as probabilities using binary logistic regression analysis. In total, 126 mandibular lateral incisors and 126 maxillary first molars were included. 46% of all mandibular lateral incisors showed complicated root canal morphology and Vertucci type III canal configuration as the most frequent. Second mesiobuccal root canal was present in 62.7%. Root canal morphologies showed high level of bilateral symmetry in both tooth groups. The probability of a subject having complicated root canal morphology in lateral incisors was significantly higher when the second mesiobuccal canal was present. Clinicians should consider all available information on patients’ root canal morphology when planning new endodontic treatment. Root canal morphology of permanent mandibular lateral incisors showed high association with the root canal morphology of permanent maxillary first molars.

Although oral ulcers represent one of the most frequent oral mucosal diseases, the available treatment is not sufficient to provide complete ulcer recovery without side-effects. Therefore, the aim of our study was to prepare a mucoadhesive oral gel based on Galium verum ethanol extract (GVL gel) and reveal its healing effects in the model of aphthous stomatitis in rats. Rats with oral ulcers were divided into the following groups: control (untreated), gel base (ulcer was treated with the gel base, three times per day for 10 days), and GVL gel group (the ulcer was treated with GVL gel in the same way as the gel base). Animals from each group were sacrificed on days 0, 3, 6, and 10 for collecting blood and ulcer tissue samples. Healing properties of oral gel were determined by clinical evaluation, as well as biochemical and histopathological examinations. Our findings suggest a significant decrease in the ulcer size in GVL gel group, with healing effects achieved through the alleviation of oxidative stress, reduction in COX-2 immunopositivity, and increase in collagen content in buccal tissue. Significant ulcer repairing potential of GVL gel highlights this oral mucoadhesive gel as a promising tool for prevention and treatment of RAS.

This study aimed to evaluate the prevalence of oval-shaped root canals at the apical, medial and coronal cross sections of all tooth groups using CBCT and image analysis software. Based on the diameter values, the long/short diameter ratio was calculated and each root canal was classified regarding its shape. Oval-shaped canals were present in 45% at the apical, 50% at the medial and 56% at the coronal level of all teeth. The mandibular central incisors presented oval canals in 24%, long oval in 28% and flat in 2% at the apical level. Mandibular molars showed a statistically significant difference (P < 0.05) in the distribution of oval-shaped canals between cross-sectional levels. Analysis of CBCT scans with an image analysis software could be a reliable and reproducible method and a valuable tool for objective determination of root canal shape in further research.

Recurrent aphthous ulcers are common painful mucosal conditions affecting the oral cavity. Despite their high prevalence and knowledge of many etiological factors, the exact mechanism of disease is still not fully understood. There are three different clinical forms of recurrent aphthous stomatitis and the therapy differs depending on it. The aim of this article is to provide an overview of current knowledge about the possible causes and treatment of recurrent aphthous stomatitis. We discuss its pathogenesis which is currently the least explained in the literature. This review offers scientists and clinicians useful information about clinical presentation, diagnosis, predisposing aetiological factors and recent trends in the management of recurrent aphthous stomatitis.

Direct pulp capping is a procedure where materials are placed on exposed vital pulp tissue in order to stimulate reparative dentinogenesis and preserve pulp vitality. Carious pulp exposure and direct pulp capping are usually accompanied by pulp inflammation which can affect pro- and anti-oxidative systems locally or systemically. Therefore, this study aimed to investigate the potential influence of direct capping of inflamed rat dental pulp with calcium hydroxide (CH) and mineral trioxide aggregate (MTA) on parameters of systemic oxidative status. Dental pulps of the first maxillary molars of Albino rats (n=32) were exposed and capped with either CH (n=8), MTA (n=8) or were left untreated (n=8). Animals with healthy pulp were used as a healthy control (n=8). After four weeks, animals were euthanized and blood samples were collected for biochemical analysis of parameters of systemic oxidative stress by spectrophotometric method. Untreated control had the significantly higher (p <0.05) values of pro-oxidative parameters and lower (p <0.05) values of anti-oxidative parameters (superoxide dismutase and reduced glutathione) compared to healthy control. CH and MTA groups showed reduced values of pro-oxidative parameters compared to untreated control and values of anti-oxidative parameters comparable to healthy control. Pulp exposure led to disbalance in systemic oxidative parameters while direct pulp capping with calcium hydroxide and mineral trioxide aggregate restored the levels of systemic oxidative parameters to that of animals with healthy dental pulp. These results indicate the importance of direct pulp capping and the potential influence of untreated inflamed pulp on systemic health.

The pro-apoptotic effect of new 2-thioxo-4-thiazolidinone derivative Les-3331 on MCF-7 and MDA-MB-231 cell lines ..

This study aimed to evaluate the applicability of the Kvaal method in determining the age of a Serbian population. In this retrospective descriptive study, 170 dental patients (age range 16–77 years) were included. The measurements of six types of teeth were performed on digital orthopantomography radiographs according to the Kvaal method. Statistical inter-observer and intra-observer reliabilities were calculated to evaluate the reproducibility of the measurements, as well as to examine the correlation between chronological age and measured values. The evaluation revealed a substantial difference (over 12 years) between chronological and estimated age. The P ratio had the strongest correlation with chronological age for the maxillary central (r = ±0.293) and lateral incisors (r = ±0.240), whereas the pulp/root width ratio at level A had the strongest correlation for the mandibular first premolars (r = ±0.270). The lowest SD values, for measurements on teeth 15/25 (SD = 125,787), had the most accurate correlation with age. Thus, we can conclude that the original Kvaal method is not applicable in the age estimation of the Serbian population. For future research, we suggest using cone beam computer tomography as a precise technology to evaluate the applicability of Kvaal and other methods for age estimation.