Conservation programs in urban ecosystems need to determine the genetic background in populations of urban dwellers. We examined the genetic diversity and structure of Pieris rapae and P. melete using AFLP markers, and compared them between species and between urban and rural environments. As a result: (i). in both species, there was no reduction in genetic diversity within urban populations by direct comparison of diversity measurements, although the analysis of molecular variance suggested significant reductions in the variance within seasonal subpopulations in urban populations; (ii). P. rapae retained greater genetic diversity within species and populations; (iii). populations of both species showed significant genetic differentiation, and P. melete was more strongly subdivided; (iv). in both species, geographically close populations did not cluster with one another in the upgma analysis; (v). there was no genetic isolation due to geographical distance in either species; (vi). the genetic composition of seasonal subpopulations differed in urban populations of both species, and the genetic distances among subpopulations were correlated with seasonal differences in P. rapae and with temporal differences in P. melete. These results indicate that the genetic diversity in urban populations of both species was reduced at times, but was maintained by dispersal from genetically differentiated populations. Differences in the ability and mode of dispersal in the two species may be reflected in the degree of population subdivision and patterns of seasonal change in the genetic composition.
Cyclizations of N-substituted N-allyltrichloroacetamides, where the substituent is an alkyl, Cbz, Boc, Ts, or Ms group, are catalyzed by a 1:1 mixture of CuCl and bipyridine to give the corresponding d,7-trichlorinated -lactams in high yields. The reactions proceed at temperatures from -78 °C to room temperature. Cyclizations of N-allyltrichloroacetamides of acyclic secondary allylic amines are achieved with good selectivity; the cis/trans ratios of the -lactams formed were dependent on the substituents on the nitrogen atom. The stereochemical outcome is compared with that of freeradical cyclization.
A combination of genetic algorithm and discrete differential dynamic programming approach (called GA-DDDP) is proposed and developed to optimize the operation of the multiple reservoir system. The demonstration is carried out through application to the Mae Klong system in Thailand. The objective of optimization is to obtain the optimal operating policies by minimizing the total irrigation deficits during a critical drought year. The performance of the proposed algorithm is compared with the modified genetic algorithm. The results show that the proposed GA-DDDP provides optimal solutions, converging into the same fitness values within a short time. The GA is able to produce satisfactory results that are very close to those obtained from GA-DDDP but required alot more computation time to obtain the precise results. The difficulties in selecting optimal parameters of GA as well as finding a feasible initial trial trajectory of DDDP are significant problems and time-consuming. The significant advantage obtained from GA-DDDP is saving of computational resource as GA-DDDP requires no need for optimizing parameters and deriving feasible initial trial trajectories. Because DDDP is a part of GA-DDDP, the good performance of GA-DDDP is obtained when applied to a small system where numbers of discretizations and variables have no influence to the dimensionality problem of DDDP.
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