BACKGROUND
Glioblastoma multiforme (GBM) is the most common and aggressive primary brain tumor with a dismal prognosis. Despite intensive study on tumor biology, the underlying mechanisms of the unlimited proliferation and progressive local invasion are still poorly understood and no effective treatment has been developed for GBM patients.
AIMs
We determine the role of TRPM7 channels in the growth, migration, and infiltration of malignant glioma cells.
METHODS
Using a combination of RT-PCR, Western blot, and patch-clamp techniques, we demonstrated the potential expression of functional TRPM7 channels in A172 cells, a human glioma cell line, as well as in human glioma tissues. Furthermore, we evaluated the role of TRPM7 in growth, migration, and infiltration in A172 cells with MTT and transwell migration and invasion assays.
RESULTS
We showed the expression of functional TRPM7 channels in both A172 cells and human glioma tissues. Suppression of TRPM7 expression with TRPM7-siRNA dramatically reduced the proliferation, migration and invasion of A172 cells. Pharmacological inhibition of TRPM7 channel with 2-aminoethoxydiphenyl borate (2-APB) shows a similar effect as TRPM7-siRNA.
CONCLUSION
We demonstrate that human glioma cells express functional TRPM7 channel, and that activation of this channel plays an important role in the proliferation, migration and invasion of malignant glioma cells. TRPM7 channel may represent a novel and promising target for therapeutic intervention of malignant glioma.
Defective sperm-ZP interaction is a major mechanism of male infertility. DZPIAR is more frequent than DSZPB in subfertile men with either normal or abnormal semen, suggesting that sequential sperm-ZP interaction tests are essential to detect these sperm defects.
Based on diatomite as modifier, maleic anhydride grafted polypropylene as compatibilizer, wood flour as reinforcing material, through single screw extruder, diatomite based wood plastic composite was prepared. The results show that: compared with one-grade-diatomite (OGD) and two-grade-diatomite (TGD), mechanical properties of the composites prepared by calcined diatomite (CD) are better, tensile strength and impact strength reached 46.9MPa and 20.1MPa. With respect to the diatomite types, it is more significant for the effects of MAPP mass on the mechanical properties of composites. The amount of MAPP was controlled in 4%, the mechanical properties of composites are better. The study of rheological properties showed that, the flow property of composites produced by calcined diatomite is better; the interface of the composite components is closer and more superior processability.
This study used Ag-embedded nanoTiO2, xylan and water-soluble Chitosan as antibacterial agents, respectively prepared shutter blades through the treating solution of the different concentration and the different drug loading amount of the poplar veneer. Through a single factor experiment, this paper analyzes that the different antibacterial agent, concentration of antibacterial agent and the drug loading amount have an effect on the antibacterial properties of the shutter blades. The results show that the order of antibacterial performance of the shutter blades impregnated antibacterial agents is the Ag-embedded nanoTiO2, Chitosan, Xylan. Comprehensiv-ely thought the antibacterial properties and economic index, the optimal concentration of the Ag-embedded nanoTiO2 impregnation solution is 1%.
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