Reinforced Concrete (RC) beams in the building structures are usually assumed as a ‘T’-shaped beam when the effects of adjacent slab flange are considered. However, experimental studies on residual flexural behavior of ‘T’-shaped RC beams are rarely reported. The residual flexural behavior of two RC rectangular beams and one RC ‘T’-shaped beam after exposure to ISO834 standard fire were experimentally investigated in the paper. The detailed experimental results, including the measured temperatures, flexural capacity, load-deflection curves and concrete strain are presented. The effects of flange on temperature distribution and residual flexural behavior of RC beams were analyzed. The experimental results show that: (a) the assumption of plane section is applicable for RC ‘T’-shaped beam. (b) The flexural capacity of RC rectangular beam was decreased 18.5% after exposure to fire for 2 hours, but the ultimate mid-span deflection was increased 55.1%. (c) The residual flexural capacity and the ultimate mid-span deflection of RC rectangular beam after fire was respectively increased 8.4% and 9.9% due to the effect of flange.
The main factors that influence product color design include the regional environmental and cultural differences that lead to people’s color preference, the tendency of popular colors as well as the symbolization of colors. The auxiliary decision system of the product color design, which is based on kansei engineering, is mainly investigated with the quantitative methodology. The variation of the preference of regional color, especially the tracking of popular color image for the public is recorded. Moreover, combined with qualitative research analysis, setting up a continuous color design auxiliary system can assist designers to coordinate the relationship of the three factors and deploy the color design job. This essay takes the color research of the cupboard as an example to explore the establishment and application of this auxiliary system.
In this paper, the superoxide dismutase fromMartianus dermestoidesis purified by the following methods: heat treatment, polyethylene concentration, Sephadex G-75 gel filtration, and DEAE-Sepharose FF ion exchange chromatography. The result shows that the purification multiple is 3.86, the activation yield is 21.89% and the specific activation of the enzyme is 447.6 U/mg. The purified SOD appears to be a sole protein on SDS-PAGE and the molecular weight is estimated to be 40.58 kDa. H2O2can obviously inhibit the enzyme activation and CHCl3-CH3CH2OH only demonstrates basically no inhibitory effect. The type of the dermestoides SOD might be Cu/Zn-SOD. After purification, some enzymatic characterizations of the SOD are studied. The optimum reaction temperature of purified SOD is 50°C. The optimum reaction pH value of purification is 6. The dermestoide SOD has a preferable stability below 50°C and at pH values between 5-8.
Dyes are usually difficult to be decolorized due to their complex chemical structures. In this work, laccase was purified from the white rot fungus Cerrena unicolor to evaluate its application in dye decolorization. SDS-PAGE analysis showed the purified laccase to be a monomeric protein of 63.7 kDa. The optimum pH for the oxidation of 2,2-azinobis-(3-ethylbenzthiaoline-6-sufonic acid) (ABTS) was 2.2 and the optimum temperature was 50°C. The activity of the purified enzyme was strongly inhibited by sodium azide and partially inhibited by cysteine, dithiothreitol. The Km values of the purified laccase for the substrate ABTS, syringaldazine and 2,6-dimethoxyphenol were 0.217, 0.306 and 0.199 mmol/L.
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