Abstract:Objective: This study aims to evaluate the eff ect of soybean milk containing a combination of anti-Streptococcus mutans IgY and chitosan to the colonization of S. mutans in the saliva and to the IgY persistency in the saliva. Materials and Methods: Experimental malnourished Sprague-Dawley rats were fed with soybean milk that is enriched with anti-S. mutans IgY and chitosan. After 15 days of feeding, we evaluated the S. mutans in dental biofi lm, in addition to the persistency level of anti-S. mutans IgY. Results: The rats that received soybean milk supplemented with anti-S. mutans IgY had the lowest number of S. mutans colonies (p < 0.05). Anti-S. mutans IgY was detected in saliva after 15 days of feeding. Conclusions: Soybean milk supplemented with anti-S. mutans IgY and chitosan could signifi cantly reduce S. mutans biofi lm, and the supplemented anti-S. mutans IgY persisted in these rats' saliva following the feeding period.
Saliva and Streptococcus mutans play role in biofilm formation. Saliva and S.mutans virulence are different between subjects with and without caries. objective: The aim of this study was to evaluate the effects of autolog saliva on biofilm formation of S. mutans isolated from caries and caries-free subjects. materials and methods: Saliva and plaque samples are obtained from caries and caries-free subjects. Plaque samples were cultured on TYS20B for 3 days. Selected colonies were picked and cultured on TSB for 3 days. After colony counting, biofilm assay was conducted and inoculated for one day. The biofilm was tested using crystal violet binding assay and quantified by measuring the optical density at 655 nm wavelength. result: The optical density of S. muttans biofilm isolated from subjects with caries were different from taste with no caries. Biofilm formation of S. muttans isolated from caries and caries-free subjects with and without the presence of autolog saliva were different. conclusion: Autolog saliva influences S. mutans biofilm formation and there is a tendency that is higher than those from subjects with no caries.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.