Effects of soybean milk, chitosan, and anti-Streptococcus mutans IgY in malnourished rats’ dental biofilm and the IgY persistency in saliva

Abstract

Abstract:Objective: This study aims to evaluate the eff ect of soybean milk containing a combination of anti-Streptococcus mutans IgY and chitosan to the colonization of S. mutans in the saliva and to the IgY persistency in the saliva. Materials and Methods: Experimental malnourished Sprague-Dawley rats were fed with soybean milk that is enriched with anti-S. mutans IgY and chitosan. After 15 days of feeding, we evaluated the S. mutans in dental biofi lm, in addition to the persistency level of anti-S. mutans IgY. Results: The rats that received soybean milk supplemented with anti-S. mutans IgY had the lowest number of S. mutans colonies (p < 0.05). Anti-S. mutans IgY was detected in saliva after 15 days of feeding. Conclusions: Soybean milk supplemented with anti-S. mutans IgY and chitosan could signifi cantly reduce S. mutans biofi lm, and the supplemented anti-S. mutans IgY persisted in these rats' saliva following the feeding period.

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

“…An interesting study carried by Bachtiar et al evaluated the effect of soybean milk containing IgY against the cariogenic bacterium Streptococcus mutans in rats. The animals fed with this milk presented a decrease in the number of S. mutans in dental biofilm and fifteen days after milk intake IgY could still be detected in the saliva [71]. In other research, the effect of specific IgY against the cell-associated glucosyltransferase enzyme (CA-gtf), of S. mutans, was evaluated.…”

The effect of coating chitosan on Porphyromonas gingivalis biofilm formation in the surface of orthodontic mini-implant

“…This phenomenon may happen because the antibacterial properties of chitosan are bacteriostatic by destabilizing the cell membrane without killing bacteria. [ 32 33 34 ] Thus, the number of P. gingivalis colonies formed on the implant surface does not necessarily decrease because what happens is only a decrease in the bacteria's virulence only. [ 35 ]…”

Analysis of IgA Anti-S mutans Serotype c and e in ECC Patients and Its Correlation towards Saliva Viscosity and DMFT Score

“…5% (w/v) Blocking buffer contains skim milk in PBS (Oxoid), 0.1% (v/v) Tween 20 (Sigma), PBS with 0.1% (v/v) Tween 20 (Sigma). Secondary antibody, Anti-Human IgA HRP (AB CAM) was used at 1:1000 concentration [12,13].…”