Bmo, 60, 1991: 225-230.Parameters of locomotor activity of mice of two strains (conventional male [CBA x C57BL] 10 Fi mice and Conventional random breed male ICR mice) are reported together with their per cent mortality after administration of isoprenaIin (ISO) at 400 mg • kg-i, 700 mg . kg-i and 750 mg/kg body mass in mice aged 3 months and at 400 mg . kg-i body mass in animals aged 5 months. Age-dependent changes in body mass and myocardial mass were assessed. It is concluded that mice of the two strains differed in their sensitivity to ISO. The o:served higher resistance of (CBA x C57BL/I0) Fl mice to ISO toxicity can be related to their higher locomotor activity and lower I::ody mass. Isoprenalin, mice, myocardium, staircase testThe sensitivity to isoprenalin (ISO) is conditioned by a number of factors the quality of which varies from species to species (Venault et aI. 1986). Of particular importance is the cardiotoxic effect of ISO inducing myocardial lesions of coagulative myocytolysis (COAM) type with subsequent development of necroses (Milei et al. 1978) within a very short period of time. Therefore ISO is used mostfrequently in experiments designed to induce primary cardiomyopathy. In response to its application Ca++ and Na+ ions in the myocardium increase rapidly, the energy conditions are affected, etc. In addition to the direct effect on the myocardium the process involves the mechanism of release of free radicals and enhanced lipid peroxidation (Ohta et al. 1986).In conventional male (CBA x C57BL/I0) Fi mice no significant biochemical or morphological myocardial changes have been found after administration of ISO in doses of 10 to 100 mg. kg-I body mass which are used routinely in rats (Fleckenstein et aI. 1977;Faltova et al. 1983b). Mice of the ICR strain exhi ited in our previous experiment higher sensitivity to ISO. One of the factors underlying this different resistance of conventional male (CBA x C57BL/1O) Fl mice might be their different locomotor activity. The present study was therefore designed to compare the effects of ISO on conventional male (CBA x C57BL/I0) Fl mice together with their locomotor activity with the corresponding data obtained for ICR mice. Materials and MethodsThe experimental animals were 60 conventional male (CBA x C57BL/I0) Fl mice (breeding colony of Institute of Biophysics, Czechoslovak Academy of Sciences, Bmo) aged 3 months and having 33.3 ± 1.8 g in body mass and 50 conventional random breed male ICR mice (breeding colony of VELAZ, Prague, Czechoslovakia) aged 3 months and having 37.4 ± 3.0 g in body mass. One week before the experiment the mice were transferred to the laboratory and kept there
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