Canine transmissible venereal tumour (CTVT) is frequently reported in dogs and is responsible for high morbidity rates and economic losses. Three clinical cases were presented at the clinic of the Faculty of Veterinary Medicine, University of Tripoli. One male and two female German shepherds were diagnosed with CTVT based on case history and tumor shape. The diagnosis was confirmed by histopathological examination. The dogs were treated with vincristine intravenously at a dose of 0.025 mg/kg and recovered fully within 4 weeks. All three dogs remained alive with no evidence of recurrence. These first cases of CTVT reported from Libya show the importance of combining case history, clinical examination and laboratory confirmation to arrive at a definitive diagnosis and implement effective therapy.
This study was designed to evaluate the reproductive performance of Japanese black cows following the 3rd injection of gonadotropin releasing hormone (GnRH) analogue administered concurrently with Ovsynch-based treatment on day 6 (day 1 = the day of ovulation). In Experiment 1, 12 cows were allocated into three groups: a control group that was subjected to Ovsynch treatment and then injected with a placebo on day 6; group 1 (Ovsynch + GnRH), which was subjected to Ovsynch treatment and was injected with GnRH analogue on day 6, and group 2 (Ovsynch + controlled internal drug-release (CIDR) + GnRH), which received Ovsynch-CIDR treatment and was injected with GnRH analogue on day 6. Blood collection and ultrasonographic observation of the ovaries were conducted daily. Both treatments induced the formation of an accessory corpus luteum and significantly increased the cross-sectional area of the luteal tissue when compared to the control. However, plasma progesterone (P4) was significantly higher in the treatment groups than in the control group on days 11, 12, 17 and 18 in the group 1 and from day 10 to 21 in the group 2. In Experiment 2, 41 cows were assigned to the same three groups described above and then artificially inseminated on day 1. The pregnancy rates on day 45 did not differ among groups. In conclusion, administration of GnRH analogue on day 6 following Ovsynch-based treatment did not improve the reproductive performance of Japanese black cows, even though the P4 concentration was higher in groups that received the GnRH.
Bmo, 60, 1991: 225-230.Parameters of locomotor activity of mice of two strains (conventional male [CBA x C57BL] 10 Fi mice and Conventional random breed male ICR mice) are reported together with their per cent mortality after administration of isoprenaIin (ISO) at 400 mg • kg-i, 700 mg . kg-i and 750 mg/kg body mass in mice aged 3 months and at 400 mg . kg-i body mass in animals aged 5 months. Age-dependent changes in body mass and myocardial mass were assessed. It is concluded that mice of the two strains differed in their sensitivity to ISO. The o:served higher resistance of (CBA x C57BL/I0) Fl mice to ISO toxicity can be related to their higher locomotor activity and lower I::ody mass. Isoprenalin, mice, myocardium, staircase testThe sensitivity to isoprenalin (ISO) is conditioned by a number of factors the quality of which varies from species to species (Venault et aI. 1986). Of particular importance is the cardiotoxic effect of ISO inducing myocardial lesions of coagulative myocytolysis (COAM) type with subsequent development of necroses (Milei et al. 1978) within a very short period of time. Therefore ISO is used mostfrequently in experiments designed to induce primary cardiomyopathy. In response to its application Ca++ and Na+ ions in the myocardium increase rapidly, the energy conditions are affected, etc. In addition to the direct effect on the myocardium the process involves the mechanism of release of free radicals and enhanced lipid peroxidation (Ohta et al. 1986).In conventional male (CBA x C57BL/I0) Fi mice no significant biochemical or morphological myocardial changes have been found after administration of ISO in doses of 10 to 100 mg. kg-I body mass which are used routinely in rats (Fleckenstein et aI. 1977;Faltova et al. 1983b). Mice of the ICR strain exhi ited in our previous experiment higher sensitivity to ISO. One of the factors underlying this different resistance of conventional male (CBA x C57BL/1O) Fl mice might be their different locomotor activity. The present study was therefore designed to compare the effects of ISO on conventional male (CBA x C57BL/I0) Fl mice together with their locomotor activity with the corresponding data obtained for ICR mice. Materials and MethodsThe experimental animals were 60 conventional male (CBA x C57BL/I0) Fl mice (breeding colony of Institute of Biophysics, Czechoslovak Academy of Sciences, Bmo) aged 3 months and having 33.3 ± 1.8 g in body mass and 50 conventional random breed male ICR mice (breeding colony of VELAZ, Prague, Czechoslovakia) aged 3 months and having 37.4 ± 3.0 g in body mass. One week before the experiment the mice were transferred to the laboratory and kept there
The present study aimed to examine the effects of γ-linolenic acid (GLA) supplementation to in vitro culture (IVC) medium on in vitro developmental competence, freezability and morphology of in vitro matured and fertilized bovine embryos. In vitro produced (IVP) bovine zygotes were cultured in IVC medium supplemented with 0 (negative control), 15, 31, 62, 125, 250, 500 or 1,000 ppm GLA, 250 ppm linoleic acid albumin (LAA) and without any supplement as a control. Day 6 blastocysts derived from culture control were cultured in IVC medium containing either 62, 250 GLA or 250 LAA for 24 h, and at Day 7 were subjected to freezing or morphological examination by electron microscope. GLA 15 showed a tendency to have a higher cleavage rate at Day 2 (70.3%) than other groups. The hatching rate at Day 9 in LAA (38.2%) was significantly higher than the control and all treatment groups (p<0.05), while the blastocyst rate in LAA (32.4%) did not differ from those of 15 (30.5%), 31 (27.1%), and 62 GLA (33.1%) or the control (35.1%). GLA in concentrations of 125, 250, 500, and 1,000 ppm had significantly detrimental effect on the blastocyst rate compared to 15, 31 and 62 ppm GLA, LAA, and control groups (p<0.05). In contrast, the highest post-thaw survival rate (100%) was observed in the control group (p<0.01). Large lipid droplets were observed in the cytoplasm of trophoblastic cells, even in the control, but were abundant in GLA groups. Taking the results of the study into consideration, the addition of GLA to the culture medium for IVP bovine embryos at the dose of 15 ppm increased the developmental competence of zygotes and enhanced the cleavage rate up to Day 2. However, blastulation rate and post-thaw survival were not increased when GLA was added to the culture media.
diately after sampling; the plasma samples were stored at-20°C until analysed. Double-antibody radioimmunoassay was used to determine the concentrations of plasma progesterone, using antisera to progesterone (GDN#337) (Taya and others 1985). The cross-sectional areas of corpora lutea (CLcs) in mm 2 were calculated by the following formula: CLcs (elliptical area) = π x (diameter a/2) x (diameter b/2), where a and b are the long and the short diameter of the corpus luteum, respectively. The regression rate of the CLcs was calculated by the following formula: Regression rate (%) in the CLcs at day 20 = 100-([day 20 CLcs/day 14 CLcs]) x 100). For statistical evaluation, the differences between the pregnant and non-pregnant cows were determined by the Mann-Whitney U test; P<0•05 was considered statistically significant. Six of the 14 cows were diagnosed as pregnant and eight cows were diagnosed as non-pregnant on day 30 by ultrasonography. The largest CLcs was observed on day 14 in both the pregnant and non-pregnant cows. In the non-pregnant cows, the mean (sd) regression rate of the CLcs was 39•2 (13•1) per cent between days 14 and 20, and 52•7 (13•7) per cent between days 14 and 21. In the pregnant cows, the respective regression rates were 3•6 (8•1) per cent and 3•4 (10•2) per cent. In the non-pregnant cows, the CLcs began to decrease from day 16; during days 18 to 23, the CLcs was significantly smaller (P<0•05) than on day 14. In the pregnant cows, there was no regression in CLcs between day 14 and day 23 (Fig 1). The regression in the CLcs was significantly different between the pregnant and non-pregnant cows from day 18 to day 23 (P<0•05). The plasma progesterone concentration was significantly higher in the pregnant cows than the non-pregnant cows between day 18 and day 23 (P<0•05). In the non-pregnant cows, the progesterone concentrations measured during days 12 to 16 were significantly (P<0•05) higher than those measured during days 20 to 23 (Fig 2). In the pregnant cows,
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