Despite previous research efforts in the fields of histology and cell physiology, the relationship between chromatin structural organization and nuclear shape remains unclear. The aim of this research was to test the existence and strength of correlations between mathematical parameters of chromatin microarchitecture and roundness of the nuclear envelope. On a sample of 240 nuclei of adrenal zona fasciculata cells stained using the DNA-specific Feulgen method, we quantified fractal parameters such as fractal dimension and lacunarity, as well as textural parameters such as angular second moment (ASM), entropy, inverse difference moment, contrast, and variance. Circularity of the nuclear envelope was determined from the nuclear area and perimeter. The results indicate that there is a statistically significant negative correlation between chromatin ASM and circularity. Moreover, there was a statistically significant positive correlation between chromatin fractal dimension and envelope circularity. This is the first study to demonstrate these relationships in adrenal tissue, and also one of the first studies to test the connection between circularity and fractal and gray-level co-occurrence matrix parameters in DNA-specific Feulgen stain. The results could be useful both as an addition to the current knowledge on chromatin/nuclear envelope interactions, and for design of future computer-assisted research software for evaluation of nuclear morphology.
Introduction/Objective Cesarean section birth rate has been constantly increasing worldwide over the last decades. The complications of cesarean section that require relaparotomy are rather serious and relatively rare. The aim of this paper is to present the incidence of surgical complications after Cesarean section at the Clinic of Gynecology and Obstetrics, Clinical Center of Serbia, Belgrade, Serbia, during a three-year period (2013-2015). Methods This is a retrospective study. Data obtained from the medical records/histories were used and processed according to descriptive statistical methods. Results During the observed period, relaparotomy was necessary in 29 (0.44%) women who had a CS. Relaparotomy was performed due to clinically and ultrasonographically evidenced hematoma of the anterior abdominal wall, retroperitoneal hematoma, hemoperitoneum, and development of hemorrhagic shock, complete wound dehiscence or diffuse peritonitis. There were no lethal outcomes after CS followed by these complications at the Clinic of Gynecology and Obstetrics, Clinical Center of Serbia in Belgrade. Conclusion The incidence of relaparotomy in our study is similar to other tertiary institutions, as well as the indications for relaparotomy. While generally observed mortality rate after post-cesarean relaparotomy in developed countries is 2.7%, in our study there were no lethal outcomes.
Rare diseases affect less than 1 in 2000 or 5 in 10,000 people by definition. Most of those diseases have genetic basis (80% of cases) and first symptoms appear in early childhood (50% of cases). Most of these diseases are chronic and degenerative and pharmacotherapy is not available for many of them. Until today, there are more than 7000 rare diseases. In Serbia, the problem of diagnosis and pharmacotherapy of rare diseases is currently under public scrutiny. Patients who suffer from rare diseases in Serbia face many challenges in terms of awareness, timely diagnosis, and adequate treatment. These people are often misdiagnosed or the diagnosis is delayed due to several problems: lack of awareness among medical professionals, lack of expertise, unavailability and/or high costs of diagnostic tests, etc. According to the National Organization of Patients with Rare Diseases in Serbia (NORBS), many diagnostic procedures have to be conducted abroad and the process comprises many difficulties: high costs, travel expenses, or transportation of biological material. Although national legislation ensures the availability of drugs for those diseases, pharmacotherapy is faced with many problems. In this work, we aim to show that improvement of the knowledge regarding rare diseases among both professionals and patients represents a crucial step for enhancement of perspectives for those patients in our community.
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