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Abstract. Plant volatiles can synergize the response to moth pheromone. Synthetic pheromone analogs, in turn, have the opposite effect in reducing pheromone attractiveness. To determine how these two types of stimuli interact and infl uence male moth behaviour, we performed wind tunnel experiments on the grapevine moth, Lobesia botrana. We noticed that a blend of host plant volatiles [(E)-β-caryophyllene, 1-hexanol, (Z)-3-hexenyl acetate and 1-octen-3-ol in a 100:20:10:5 ratio] signifi cantly increased the response of males to an optimized blend of sex pheromone [(7E,9Z)-dodeca-7,9-dienyl acetate (E7,Z9-12:Ac), (7E,9Z)-dodeca-7,9-dienol (E7,Z9-12:OH) and (Z)-9-dodecenyl acetate (Z9-12:Ac)] in a 100:10:2 ratio. However, the response of males to the natural attractant was signifi cantly reduced by two analogs [(9E,11Z)-tetradeca-9,11-dien-2-one (MK 2) and [(9E,11Z)-1,1,1-trifl uoro-tetradeca-9,11-dien-2-one (TFMK 3)], of the major component of the sex pheromone of the insect (E7,Z9-12:Ac). When both stimuli were tested on males at pheromone:analog:plant volatile blend 1:100:1000 ratio, the plant blend offset the inhibitory effect induced by TFMK 3 but not that of MK 2. Our results show for the fi rst time that under laboratory conditions plant volatiles can prevent inhibition by a pheromone analog.

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Objective To describe the acute cardiovascular adaptation of the fetus after connection to an artificial placenta (AP) in a sheep model, using ultrasound and invasive and non-invasive hemodynamic assessment. MethodsThis was an experimental study of 12 fetal sheep that were transferred to an AP system, consisting of a pumpless circuit with umbilical cord connection, at 109-117 days' gestation. The study was designed to collect in-utero and postcannulation measurements in all the animals. The first six consecutive fetuses were fitted with intravascular catheters and perivascular probes to obtain invasive physiological data, including arterial and venous intravascular pressures and perivascular blood flows, with measurements taken in utero and at 5 and 30 min after cannulation. These experiments were designed with a survival goal of 1-3 h. The second set of six fetuses were not fitted with catheters, and experiments were aimed at 3-24 h of survival. Echocardiographic assessment of cardiac anatomy and function, as well as measurements of blood flow and pre-and postmembrane pressures recorded by circuit sensors in the AP system, were available for most of the fetuses. These data were acquired in utero and at 30 and 180 min after cannulation. Sanchez-Martinez et al. ResultsCompared with in-utero conditions, the pulsatility index at 30 and 180 min after connection to the AP system was reduced in the umbilical artery (median, 1.36 (interquartile range (IQR), 1.06-1.50) vs 0.38 (IQR, 0.31-0.50) vs 0.36 (IQR, 0.29-0.41); P < 0.001 for extreme timepoints) and the ductus venosus (median, 0.50 (IQR, 0.41-0.67) vs 0.29 (IQR, 0.22-0.33) vs 0.36 (IQR, 0.22-0.41); P = 0.011 for extreme timepoints), whereas umbilical venous peak velocity increased (median, 20 cm/s (IQR, 18-22 cm/s) vs 39 cm/s (IQR, 31-43 cm/s) vs 43 cm/s (IQR, 34-54 cm/s); P < 0.001 for extreme timepoints) and flow became more pulsatile. Intravascular monitoring showed that arterial and venous pressures increased transiently after connection, with median values for mean arterial pressure at baseline, 5 min and 30 min of 43 mmHg (IQR,(35)(36)(37)(38)(39)(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50)(51)(52)(53)(54), 72 mmHg (IQR, 61-77 mmHg) and 58 mmHg (IQR,(50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64), respectively (P = 0.02 for baseline vs 5 min). Echocardiography showed a similar transient elevation of fetal heart rate at 30 and 180 min after connection compared with in utero (median, 145 bpm (IQR,(142)(143)(144)(145)(146)(147)(148)(149)(150)(151)(152)(153)(154)(155)(156) vs 188 bpm (IQR, vs 175 bpm (IQR,; P = 0.001 for extreme timepoints). Fetal cardiac structure and function were mainly preserved; median values for right fractional area change were 36% (IQR, 34-41%) in utero, 38% (IQR, 30-40%) at 30 min and 37% (IQR, 33-40%) at 180 min (P = 0.807 for extreme timepoints).Conclusions Connection to an AP system resulted in a transient fetal hemodynamic response that tended to normalize over hours. In this short-term evaluation, cardiac structure and...

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